Abstract: Sub-diffraction limited fluorescent images using a fiber-based stimulated emission depletion (STED) microscope are reported. Both excitation and depletion beams are transported through polarization-maintaining fiber and a lateral resolution of 100 nm has been achieved.

Abstract: The invention provides methods of inhibiting the growth or metastasis of a cancer in a mammal by inhibiting a Ral GTPase in the mammal. The invention also provides small molecule inhibitors of Ral GTPases useful in the methods of the invention and pharmaceutical compositions containing the therapeutically effective compounds of the invention, and methods of using the same.

Abstract: According to some aspects of the present disclosure, an atomic clock and methods of forming and/or using an atomic clock are disclosed. In one embodiment, an atomic clock includes: a light source configured to illuminate a resonance vapor cell; a narrowband optical filter disposed between the light source and the resonance vapor cell and arranged such that light emitted from the light source passes through the narrowband optical filter and illuminates the resonance vapor cell. The resonance vapor cell is configured to emit a signal corresponding to a hyperfine transition frequency in response to illumination from the light source, and a filter cell is disposed between the light source and the resonance vapor cell and configured to generate optical pumping. An optical detector is configured to detect the emitted signal corresponding to the hyperfine transition frequency.

Abstract: Methods of treating or preventing microbial infection in a subject in need of treatment by administering a therapeutically effective amount of ergothioneine, or functional analog, or prodrug, or salt thereof. Ergothioneine may be advantageously administered in conjunction with lactoferrin.

Abstract: Embodiments of the present technology include a wearable physiological monitoring device, related algorithms and software that are tied to a portable electronic device for readout. The wearable device can perform real-time measurement of a number of physiological and environmental parameters including heart rate, pulse oximetry, respiration, movement, environmental particulate matter, moisture, temperature (e.g., ambient air and body temperatures) and geospatial location. Some embodiments may establish a physiological baseline for a patient by measuring the above parameters during a healthy state. Collected data can be wirelessly transmitted to a portable electronic device or monitoring and feedback platform where software will analyze the data and make assessments of the device wearer's health based upon the wearer's baseline.

Abstract: Compounds that inhibit the activity of Signal transducer and activator of transcription 3 (STAT3), or pharmaceutically acceptable salts or prodrugs thereof, and methods of using these compounds to treat cancer and other diseases.

Abstract: A novel method of diluting the structures in the cell population, such that individual cells, dependent on the activity of the structures, become single measurement devices. This can be applied to all Bacterial Microcomparments (“BMCs”), organelles, and macromolecules, and could provide a universal method for the design of novel ones and understanding of the diverse structures. In one aspect the present invention provides A method of creating a bacterial strain with inducible and detectable carboxysomes. The method includes the steps of incorporating a labeled carbon-fixation enzyme into the genome of a bacterium; deleting all or a portion of the ccm operon from the bacterium; and reintroducing a ccm operon comprising an inducible promoter to create a ?ccm+ strain.

Abstract: Methods, systems, and devices for ultra or extreme-high vacuum are described. Such systems may comprise a vacuum chamber, a target within the vacuum chamber, two or more overlapping radiation shields arranged within an inner vacuum space of a vacuum chamber, and surrounding at least a portion of the target, a first and a second cooling element unit thermally coupled to a first and second radiation shield of the two or more overlapping radiation shields, wherein the first unit is configured to reduce the first radiation shield's temperature to at least <100K, and the second unit is configured to reduce the second radiation shield's temperature to at least <25K, and a third cooling element unit coupled to the target and isolated from the first and second radiation shield, wherein the third cooling element unit is configured to reduce the target's temperature to at least <4K.

Abstract: The present invention relates to a medical implant, and more particularly, to a vascular implant having a dual coating structure for preventing in-stent restenosis and thrombosis. In one embodiment, the invention contemplates a vascular stent with a coating comprising a hydrophobic, degradable core with a coaxial sheath comprising at least one polyethylene-glycol derivative. In one embodiment, the at least one polyethylene-glycol derivative comprises polyethylene-glycol dimethacrylate.

Abstract: The present invention describes peptides, peptides carriers, peptide nanobodies, and peptide-drug covalent conjugates having efficient cell and tissue penetration. The peptides and associated configurations can be used in covalent attachments or as complexes or nanoparticles in conjunction with therapeutic agents to enhance their tissue, cellular, and intracellular delivery. Also, the peptides and associated configurations can enhance binding to negatively charged matrices in the body for improved localization or retention of therapeutic carriers and agents.

Abstract: The present disclosure relates generally to methods and systems useful in imaging applications, especially biological imaging applications, and applications in the metrology, atmospheric, scientific and medical fields. In one aspect, the disclosure provides a method of imaging an object, including illuminating the object with incident radiation through one or more adaptive optical elements; receiving transmitted radiation from the object at a photodetector to provide a base image; and performing the following steps one or more times: adjusting the one or more adaptive optical elements, the adjustment including modifying an optical transfer function of the one or more adaptive optical elements, and receiving transmitted radiation from the object at the photodetector to provide an adjusted image; wherein the adjustment and receiving steps are performed until the adjusted image has substantially reduced aberrations compared to the base image.

Abstract: Through discrimination of the scattered signal polarization state, a lidar system measures a distance through semi-transparent media by the reception of single or multiple scattered signals from a scattering medium. Combined and overlapped single or multiple scattered light signals from the medium can be separated by exploiting varying polarization characteristics. This removes the traditional laser and detector pulse width limitations that determine the system's operational bandwidth, translating relative depth measurements into the conditions of two surface timing measurements and achieving sub-pulse width resolution.

Abstract: Methods of preventing or treating rheumatoid arthritis (RA) in a subject by introducing the DRB1*04:01K71E mutation that is resistant to RA. The resistant allele is introduced into the subject having or at risk of developing RA, using a HLA CRISPR/Cas9 vector that targets codon 71 in the HLA allele DRB1*04:01, introducing a single A to G point mutation in codon 71 by homology directed repair to alter the lysine at position 71 of the expressed protein to glutamic acid. This modified allele is affected in the subject's hematopoietic stem cells, which are then expanded and transplanted back into the patient. This microgene therapy confers RA-resistance via an autologous transplant. The invention includes isolated nucleic acids, vectors, recombinant viruses, cells, and pharmaceutical compositions to modify the HLA DRB1*04:01 allele.

Abstract: A method of screening a test compound, e.g., a fibrosis or IPF inhibitor, for induction of an unjammed-to-jammed transition (UJT) in fibrotic primary human bronchial epithelial cells (HBECs) isolated from a subject with a fibrotic lung disease includes culturing the fibrotic primary HBECs at an air-liquid interface for a time sufficient to provide a differentiated pseudostratified epithelium contacting the cultured cells with the test compound; and monitoring the motility of the cultured cells to identify the cultured cells as moving or stationary; wherein stationary cultured cells indicate that the test compound induces the UJT. Also included are methods of identifying lung fibrosis biomarkers.

Abstract: A method for synchronously driving more than two or more rotational tissue screws and a method for simultaneously affixing a medical device to tissue employing the synchronous drive system. The method employs a synchronous drive system is particularly configured to affix an apical cuff to cardiac muscle tissue by simultaneously driving a plurality of rotational tissue screws through the apical cuff and into cardiac muscle tissue thereby affixing the apical cuff to the cardiac muscle tissue.

Abstract: Surface polarity of a ferroelectric nematic can be configured to generate vectorial control of the orientation of its bulk polarization field. The contact between a surface with in-plane polarity and a ferroelectric nematic liquid crystal generates preferred in-plane orientation of the ferroelectric polarization field at that interface, which can lead to the formation of fluid or glassy monodomains of high polarization without electric field poling.

Abstract: A two-component biomaterial and method that replicates both the structural complexity and diverse molecular composition necessary to create a tissue's form and function. It is an objective of the current invention to use the unique combination material and methods herein to provide a pharmaceutical composition, a medical device, a tissue regeneration scaffold, as well as a scaffold for 3D organ culture (tissue on a chip, lab grown meat, research stem cell differentiation) comprising a significant amount of acellular tissue particles packed tightly and held together via crosslinking between the acellular particles and a thiolated protein.

Abstract: Functionalized zeolites, including a zeolite substrate and a self-assembled monolayer of a phosphonic acid on a surface of the zeolite substrate, are disclosed, as are methods of making and using the functionalized zeolites. The disclosed methods and compositions have various applications, including in the use of molecular sieves to separate small-molecule gases from mixtures thereof. Gas adsorption selectivities and diffusion rates of the functionalized zeolites may be tuned or selected according to the disclosed methods.

Abstract: Methods of treating, reducing the risk of, preventing, or alleviating at least one symptom of a retinal disease, injury, or condition in a subject involve administering a pharmaceutically effective amount of kynurenic acid to the subject. The kynurenic acid may be administered intravenously or intravitreally. Systems for treating retinal diseases, injuries, or conditions are also disclosed and may include an administration device.

Abstract: A wideband amplifier includes a first diplexer receiving broadband input signals and divides them by frequency into a low band input signal and a high band input signal. The amplifier has separate high band and low band amplifiers coupled to amplify the low and high band input signals, and a second diplexer coupled to combine outputs of the low and high band amplifiers to form a wideband output. A method of amplification of an input signal includes separating the input signal into high and low band signals, separately amplifying the high and low band signals, and combining amplified high and low band signals into an output signal.