Abstract: The present invention provides a non-naturally occurring microbe capable of growing in a medium comprising methanol, comprising a heterologous polynucleotide encoding a heterologous methanol dehydrogenase (MDH) derived from a Corynebacterium organism (Cor), wherein the MDH is expressed in the microbe, and wherein the MDH exhibits a Km of no more than 3 mM for methanol. Also provided are uses of the non-naturally occurring microbe for oxidizing methanol and producing a metabolite as well as the preparation of the non-naturally occurring microbe.

Abstract: A responsive hydrogel-based material may be used as a carrier system for the in situ delivery of various cargo substances, including bioactive moieties. The hydrogel structure, which includes photodegradable and thioether moieties in its three dimensional network, enables finely tuned local release of cargo substances as a function of the in vivo tissue environment (e.g., enzyme concentration or reducing environment) and externally applied stimuli (e.g., light) by selective spatiotemporal hydrogel degradation.

Abstract: A method for catalytically converting a dihydrotetrazine 1 into a tetrazine 2, wherein one R group on the dihydrotetrazine 1 is a substituted or unsubstituted aryl, heteroaryl, alkyl, alkenyl, alkynyl, carbonyl, or heteroatom-containing group, and the other R group is selected from the group consisting of H and substituted or unsubstituted aryl, heteroaryl, alkyl, alkenyl, alkynyl, carbonyl,—or heteroatom-containing groups; 1, 2 wherein the method comprises oxidizing dihydrotetrazine 1 in a reaction medium in the presence of a catalyst and a stoichiometric oxidant.

Abstract: Spectral imaging sensors and methods are disclosed. One spectral imaging sensor includes a light source, an array of coded apertures, one or more optical elements, and a photodetector. The light source is configured to emit a plurality of pulses of light toward an object to be imaged. The array of coded apertures is positioned to spatially modulate light received from the object to be imaged. The optical elements are configured to redirect light from the array of coded apertures. The photodetector is positioned to receive light from the one or more optical elements. The photodetector comprise a plurality of light sensing elements. The plurality of light sensing elements are operable to sense the light from the one or more optical elements in a plurality of time periods. The plurality of time periods have a same frequency as the plurality of pulses of light.

Abstract: A coating head includes a coating device and at least a first flexible blade that is capable of being shaped into contact with a solvent-containing film on a substrate upon pressing the first flexible blade against the film, thereby providing contact between the blade and the film over a contact distance when the coating head is drawn over the substrate. The coating device and the blade are separated by a spacing distance such that partial loss of solvent occurs from a starting film formed on the substrate by the coating device as the coating head is drawn over the substrate, the partial solvent loss forming the solvent-containing film. A method of producing a film having a hierarchical structure includes drawing a coating head across a substrate to form on the substrate via the coating device a starting film including a solvent and a material for forming the hierarchical structure.

Abstract: The present invention provides N-acetyl-muramic acid (NAM) derivatives having Formula I, wherein Xa is selected from the group consisting of X1-X59, Ya is selected from the group consisting of H, monophosphate, uridine diphosphate and ethyl azide linker prepared from 2-azido-ethanol, and Za is selected from the group consisting of OH, an ethylene diamine coupled fluorophore, a peptide and a peptide with an ethylene diamine coupled fluorophore, wherein the peptide is selected from the group consisting of a monopeptide, a dipeptide, a tripeptide and a pentapeptide. Also provided are methods for synthesizing NAM derivatives and methods for modulating Nod2 in cells, modifying bacterial cell wall or modulating innate immune response by a subject to bacterial cells upon exposure to NAM derivatives.

Abstract: A non-naturally occurring microbe capable of growing in a medium comprising methanol is provided. The methanol contributes to a significant percentage (e.g., at least 40%) of the carbon source for the non-naturally occurring microbe, which expresses heterologous methanol dehydrogenase (MDH) and heterologous ribulose monophosphate (RuMP) pathway enzymes. Methods for producing liquid fuels and chemicals by the non-naturally occurring microbe and methods for preparing the non-naturally occurring microbe are also provided.

Abstract: The invention relates a method for producing a stable recombinant protein, comprising growing a non-naturally occurring host cell in a culture medium to produce a recombinant protein, and making a composition comprising the recombinant protein and a polysorbate. The production of endogenous lipoprotein lipase by the host cell is reduced. The endogenous lipoprotein lipase is present in the composition in a small amount, and is capable of degrading the polysorbate. The invention also relates to the relevant host cells and compositions, and preparation thereof.

Abstract: The invention relates a method for producing a stable recombinant protein, comprising growing a non-naturally occurring host cell in a culture medium to produce a recombinant protein, and making a composition comprising the recombinant protein and a polysorbate. The production of endogenous lipoprotein lipase by the host cell is reduced. The endogenous lipoprotein lipase is present in the composition in a small amount, and is capable of degrading the polysorbate. The invention also relates to the relevant host cells and compositions, and preparation thereof.

Abstract: Spectral imaging sensors and methods are disclosed. One spectral imaging sensor includes a light source, an array of coded apertures, one or more optical elements, and a photodetector. The light source is configured to emit a plurality of pulses of light toward an object to be imaged. The array of coded apertures is positioned to spatially modulate light received from the object to be imaged. The optical elements are configured to redirect light from the array of coded apertures. The photodetector is positioned to receive light from the one or more optical elements. The photodetector comprise a plurality of light sensing elements. The plurality of light sensing elements are operable to sense the light from the one or more optical elements in a plurality of time periods. The plurality of time periods have a same frequency as the plurality of pulses of light.

Abstract: A substituted trans-cycloheptene according to formula (I); wherein: a) Z and L are each selected from the group consisting of SiR1R2, CH2, CHOH, and CHR2; R1 is phenyl or CH3; R2 is phenyl, CH3, (CH2)nCN, or (CH2)nOH, wherein n is an integer from 1 to 5; Ra and Rb are each individually selected from the group consisting of H, OH, and CH3; and Z and L are not both SiR1R2; or b) Z is BocN, L is CH2, Ra is H, and Rb is H; or c) Z is C=0, L is CH2, Ra is H, and Rb is H.

Abstract: Variable flow rate fuel ejectors, and methods of use therefore, are disclosed. One variable flow rate ejector includes a primary nozzle, a needle, a motor, a first stop portion, and a first impact-absorbing portion. The primary nozzle is connected to a first inlet chamber to receive a first fluid and transmit a flow of the first fluid through the primary nozzle opening. The needle is disposed to create a gap between the tapered portion of the needle and the primary nozzle opening. The motor is coupled to axially move the needle to vary a size of the gap. The first stop portion delimits the axial movement of the needle in a direction of retraction of the needle from the primary nozzle opening. The first impact-absorbing element is positioned to contact the first stop portion or the needle, respectively, when the needle is fully retracted from the primary nozzle opening.

Abstract: A redox flow battery is provided having a double-membrane (one cation exchange membrane and one anion exchange membrane), triple-electrolyte (one electrolyte in contact with the negative electrode, one electrolyte in contact with the positive electrode, and one electrolyte positioned between and in contact with the two membranes). The cation exchange membrane is used to separate the negative or positive electrolyte and the middle electrolyte, and the anion exchange membrane is used to separate the middle electrolyte and the positive or negative electrolyte. This design physically isolates, but ionically connects, the negative electrolyte and positive electrolyte. The physical isolation offers great freedom in choosing redox pairs in the negative electrolyte and positive electrolyte, making high voltage of redox flow batteries possible. The ionic conduction drastically reduces the overall ionic crossover between negative electrolyte and positive one, leading to high columbic efficiency.

Abstract: The present invention relates to a novel method for detecting efficient cryptic HIV replication in a patient who receives a suppressive antiviral therapy followed by administration of the HIV integrase inhibitor in an effective amount for intensifying the suppressive antiviral therapy, and has undetectable plasma viremia prior to the administration of the HIV integrase inhibitor. The method comprises making a pre-intensification measurement and one or more post-intensification measurements of the concentration of an episomal artifact in samples from the patient, and computing a pre-intensification HIV infection success ratio (R). A pre-intensification HIV infection success ratio (R) sufficiently close to 1 indicates that the patient has the efficient cryptic HIV replication. The method may further comprise quantifying the efficient cryptic HIV replication.

Abstract: In various aspects, the processes disclosed herein may include the steps of inducing an electric field about a non-conductive substrate, and depositing functionalized nanoparticles upon the non-conductive substrate by contacting a nanoparticle dispersion with the non-conductive substrate, the nanoparticle dispersion comprising functionalized nanoparticles having an electrical charge, the electric field drawing the functionalized nanoparticles to the non-conductive substrate. In various aspects, the related composition of matter disclosed herein comprise functionalized nanoparticles bonded to a surface of a non-conductive fiber, the surface of the non-conductive fiber comprising a sizing adhered to the surface of the non-conductive fiber. This Abstract is presented to meet requirements of 37 C.F.R. §1.72(b) only. This Abstract is not intended to identify key elements of the processes, and related apparatus and compositions of matter disclosed herein or to delineate the scope thereof.

Abstract: Medical treatment simulation systems and devices are disclosed. One device includes an overlay, a simulated treatment structure, at least one feedback device, and at least one processor. The overlay is configured to be secured to the live subject and to cover at least a portion of a body of the live subject. The simulated treatment structure is configured to simulate a structure associated with the medical procedure. The at least one feedback device is configured to provide a feedback signal to the live subject. The at least one processor is connected to the simulated treatment structure and the at least one feedback device. The processor is programmed to operate the feedback device to provide the feedback signal based upon input generated from interaction between a treatment provider and the simulated treatment structure. The disclosed devices may be used to simulate intravenous, catheter, defibrillation, and/or thoracic treatments.

Abstract: Medical treatment simulation devices are disclosed. One device includes an overlay configured to be secured to a subject, a simulated treatment structure, at least one detector, and at least one feedback device. The detector is coupled to the simulated treatment structure and configured to detect an interaction with the simulated treatment structure. The feedback device is coupled to the overlay and configured to provide feedback based on the interaction detected by the at least one detector.

Abstract: Disclosed are inhibitors of the USP1/UAF1 deubiquitinase complex, for example. of formula (I), wherein R1, R2, and Q are as defined herein, which are useful in treating diseases such as cancer, and improving the efficacy of DNA damaging agents in cancer treatment. Also disclosed is a composition comprising a pharmaceutically suitable carrier and at least one compound of the invention, a method of method of inhibiting a heterodimeric deubiquitinase complex in a cell, and a method of enhancing the chemotherapeutic treatment of cancer in a mammal undergoing treatment with an anti cancer agent. Further disclosed is a method of preparing compounds of the invention.

Abstract: A responsive hydrogel-based material may be used as a carrier system for the in situ delivery of various cargo substances, including bioactive moieties. The hydrogel structure, which includes photodegradable and thioether moieties in its three dimensional network, enables finely tuned local release of cargo substances as a function of the in vivo tissue environment (e.g., enzyme concentration or reducing environment) and externally applied stimuli (e.g., light) by selective spatiotemporal hydrogel degradation.

Abstract: Methods, systems, and apparatus transferring power between the grid and an electric vehicle are disclosed. The apparatus may include at least one vehicle communication port for interfacing with electric vehicle equipment (EVE) and a processor coupled to the at least one vehicle communication port to establish communication with the EVE, receive EVE attributes from the EVE, and transmit electric vehicle station equipment (EVSE) attributes to the EVE. Power may be transferred between the grid and the electric vehicle by maintaining EVSE attributes, establishing communication with the EVE, and transmitting the EVSE maintained attributes to the EVE.