Abstract: The disclosure relates, in some aspects, to compositions and methods useful for production of nitrated aromatic molecules. The disclosure is based, in part, on whole cell systems expressing artificial fusion proteins comprising cytochrome P450 enzymes linked to reductase enzymes. In some aspects, the disclosure relates to methods of producing nitrated aromatic molecules in whole cell systems having artificial fusion proteins comprising cytochrome P450 enzymes linked to reductase enzymes.

Abstract: Provided herein are methods related to co-packaging of multiple rAAV particles, e.g., by introducing multiple nucleic acid vectors encoding proteins or polypeptides or RNAs of interest into a single cell preparation.

Abstract: Provided herein are charge complementary peptides that can be optionally coupled to a cargo polypeptide that are capable of self-assembling under stimulating conditions. The charge complementary peptides can be capable of forming supramolecular structures. Also provided herein are methods of using the charge complementary peptides provided herein.

Abstract: A polymer three-dimensional (3D) printing methodology is disclosed for freeform fabrication of polymeric structures under ambient conditions without the use of printed support structures. The build material can be dissolved in a suitable solvent for 3D printing. The polymer solution can be printed in (e.g., continuously printed using a moving dispensing nozzle) a yield-stress support bath to form an intermediate article. The intermediate article may be liquid or only partially coagulated after being printed into the yield-stress support bath. The yield-stress support bath may be at least partially disposed within a container, and the container may be immersed in a post-treatment coagulation solution to remove some or all of the solvent, causing the build material to fully solidify to form a finished article from the intermediate article.

Abstract: Various examples are provided for wireless power charging for versatile receiver positions. In one example, a three dimensional array of transmitter coils is positioned around a charging area. A control circuit causes the array of transmitter coils to generate a magnetic field that charges a device with any position and orientation in the charging area.

Abstract: Described herein are fluid-manipulation-based devices and methods of use. Fluid manipulations according to devices and methods as described herein can be configured to perform assays on biological samples. Devices and methods as described herein can manipulate and analyze nanoliter volumes of fluid, microliter volumes of fluid, milliliter volumes of fluid, or greater. Embodiments of the present disclosure can enable random biological assays and rapid, simultaneous analysis of multiple biological samples.

Abstract: Embodiments of the subject invention relate to an electropermanent magnet core (EPM core) having two permanent magnets (or two permanent magnet portions where each portion can have one or more permanent magnets), including a fixed permanent magnet portion and a switching permanent magnet portion, where a switching magnetic field is used to switch the magnetization of the switching permanent magnet portion, but not switch the magnetization of the fixed permanent magnet portion.

Abstract: In an example, a system includes a plurality of acoustically coupled nodes. Each of the nodes includes a transducer, a communication circuit and a controller. The transducer is adapted to be mechanically coupled to a medium. The communication circuit is coupled to the transducer to send and receive acoustic signals via the medium according to at least one communication parameter. The controller is to adaptively configure the at least one communication parameter of the communication circuit based on an acoustic signal received from at least one other of the nodes.

Abstract: Embodiments of the present disclosure provide for methods and systems for preparing chromosomal spread for a selected cell so that chromosomal spreads and/or translocations can be correlated with the selected cell.

Abstract: Various examples of methods, systems, and apparatus are provided for monitoring using an improved mouth guard apparatus. In one example, a battery-free diagnostic mouth guard includes a biting force-voltage transducer comprising a piezoelectric film; a compact resonance tank comprising a wireless sensor; and a transmitting antenna for transmitting sensing data. A piezo-voltage from the biting force-voltage transducer can bias a varactor diode loaded on the wireless sensor whose response frequency is tuned due to a capacitance change of the varactor diode. In some implementations, an external processing equipment can wirelessly detect a frequency shift of the varactor diode integrated resonator. In another example, a method includes detecting a biting force via a biting force-voltage transducer; biasing a varactor diode with a voltage from the transducer; tuning a response frequency of a split ring resonator using a capacitance change of the varactor diode; and emitting sensing data at the response frequency.

Abstract: Various examples are provided for polishing techniques for flexible tubular workpieces. In one example, a method includes supporting a tubular workpiece on a rod that extends axially through it; positioning a turning wheel against an external surface of the tubular workpiece, where it is held by magnetic attraction; and rotating the tubular workpiece by rotating the turning wheel. The external surface of the tubular workpiece is polished by the abrasive particles during rotation of the tubular workpiece. In another example, a polishing system includes a workpiece holder including a rod configured to axially support a tubular workpiece; a turning wheel with abrasive particles distributed about an outer surface; a wheel support assembly configured to position the outer surface of the turning wheel against the an external surface of the tubular workpiece, where it is held by magnetic attraction. The external surface is polished during rotation of the tubular workpiece.

Abstract: The invention pertains to a plant cell or a plant having one or more mutations in the promoters of both the alleles for CsLOB1 gene, wherein the one or more mutations are in the promoter binding sites for PthA4 protein from Xanthomonas spp., and wherein the one or more mutations reduce or abolish the binding of the Xanthomonas spp. PthA4 protein on to the binding sites in the promoters of the CsLOB1 genes. Also, a plant cell or a plant having one or more mutations in the coding regions of both the alleles for CsLOB1 gene, wherein the one or more mutations reduce or abolish the binding of the function of CsLOB1 protein are provided. The invention further pertains to the methods of making the plant cell or the plant resistant to infection by Xanthomonas spp.

Abstract: The subject invention provides therapeutic compositions, and uses thereof for the treatment or amelioration of injury to small intestine mucosa. In preferred embodiments, the composition comprises one or more nutrients and/or electrolytes that acquire or retain considerable absorptive capacity.

Abstract: Methods and compositions for modulating repeat non-ATG protein (RAN protein) translation are provided. In some aspects, the disclosure relates to methods for treating a subject having a disease associated with RAN protein translation by administering the subject a modulator of eIF3 or an eIF3 subunit, or an antibody that bind to a RAN protein.

Abstract: In some aspects, the present disclosure provides compounds of the formula: wherein the variables are as defined herein. In some embodiments, these compounds may be used to reduce the pain of a patient. These compounds may be used in pain relief and show an improved pharmaceutical profile relative to other commonly used opiates and opioid derivatives.

Abstract: Adeno associated viral (AAV) particles are emerging as a useful vehicle for gene delivery to various organs and tissues, one of them being the retina. Provided here are variant AAV (e.g., variant serotype 2 (AAV2)) capsid proteins and variant capsid protein containing particles with enhanced ability to transduce retinal cells.

Abstract: Disclosed are next-generation multi-mutated capsid protein-modified rAAV expression vectors, as well as infectious virions, compositions, and pharmaceutical formulations that include them. Also disclosed are methods of preparing and using these high transduction efficiency vector constructs in a variety of therapeutic applications including, inter alia, as delivery agents for the treatment or amelioration of one or more diseases or abnormal conditions in an affected mammal using in vivo and/or ex situ viral vector-based gene therapy protocols. Also disclosed are large-scale production methods for the multi-mutated, capsid-modified rAAV expression vectors, viral particles, and infectious virions, as well as use of the disclosed compositions in the manufacture of medicaments for use in a variety of in vitro and/or in vivo therapeutic methodologies.

Abstract: Devices and methods for detecting credit card skimmers are provided herein. A device can include a printed circuit board having a pattern of electrical traces on its surface, and a computer readable medium comprising instructions that cause a processor to direct an electrical voltage to a trace and detect an electrical current between two points of the trace. A gap can be disposed between the two points across which a voltage is applied in order to detect a magnetic reader. A count of the detected magnetic readers within a credit card reading device may be indicative of the presence of a credit card skimmer.

Abstract: Disclosed herein is a unique surgical device that assists medical practitioners to stabilize and support their hand while conducting a surgical procedure. Specifically exemplified is a hand stabilizing device that includes a stem, having a shaft, a bent portion, and a base portion. The device may also be flexible. The device serves to support and stabilize the hand during a surgical procedure.

Abstract: Provided herein are modified recombinant adeno-associated virus (rAAV) capsid proteins, such as modified rAAV1, rAAV5, and rAAV6 capsid proteins, rAAV particles comprising such capsid proteins, nucleic acid molecules encoding such capsid proteins, as well as compositions, kits and methods of use thereof.