Abstract: The isolation, purification, cloning and expression of a papaya xylanase gene is presented. This gene can be utilized to identify other related xylanase genes from other plants, especially dicots. Once cloned, xylanase genes can be utilized to create transgenic plants exhibiting controlled growth, abscission, dehiscence and/or fruit and vegetable ripening characteristics.

Abstract: Dry homogenized metal hydrides, in particular aluminum hydride compounds, as a material for reversible hydrogen storage is provided. The reversible hydrogen storage material comprises a dry homogenized material having transition metal catalytic sites on a metal aluminum hydride compound, or mixtures of metal aluminum hydride compounds. A method of making such reversible hydrogen storage materials by dry doping is also provided and comprises the steps of dry homogenizing metal hydrides by mechanical mixing, such as be crushing or ball milling a powder, of a metal aluminum hydride with a transition metal catalyst. In another aspect of the invention, a method of powering a vehicle apparatus with the reversible hydrogen storage material is provided.

Abstract: The invention establishes that coffee fruit ripening is climacteric. The invention further provides techniques to isolate substantially pure RNA from coffee fruit even though the fruit contains high levels of phenolic compounds and carbohydrate which would otherwise interfere with obtaining clean RNA preparations from this tissue. The invention provides purified proteins, nucleic acid sequences that code on expression therefore and recombinant DNA molecules, including hosts transformed therewith, and methods for transforming coffee plants to suppress the expression of coffee fruit-expressed ACC synthase and/or coffee fruit-expressed ACC oxidase necessary for ethylene biosynthesis and the ripening of coffee fruit. Coffee plants are transformed with vectors containing coffee fruit-expressed ACC synthase and/or with ACC oxidase DNA sequences that code on expression for the respective RNA that is antisense or sense to the mRNA for the respective ACC synthase and/or ACC oxidase.

Abstract: The present invention provides compositions of matter comprising a polypeptide expressed from insect cells harboring a baculovirus vector the encodes the polypeptide, wherein the polypeptide comprises amino acid sequences derived from the p42 fragment of the Plasmodium falciparum gp 195 protein or derivatives thereof. Such compositions of matter find use for example for inducing the production of anti-p42 antibodies both in vivo and in vitro.

Abstract: A method of inhibiting the proliferation of a hyperproliferative mammalian cell having a multiple drug resistant phenotype utilizing an amount of a laulimalide compound effective to disrupt the dynamic state of microtubule polymerization and depolymerization to arrest cell mitosis is disclosed, together with laulimalide compounds which find use in such method.

Abstract: Coinjection of unfertilized mouse oocytes with sperm heads and exogenous nucleic acid encoding a transgene results in transgene-expressing embryos, reflecting nucleic acid-sperm head association before coinjection. Nonselective transfer to surrogate mothers of embryos resulting from coinjection produced offspring expressing the integrated transgene.

Abstract: The invention relates to nucleic acid sequences isolated from sugarcane and to methods of using them. In particular, the inventions relates to nucleotide sequences which are derived from sugarcane polyubiquitin genes and which are capable of directing constitutive expression of a nucleic acid sequence of interest that is operably linked to the sugarcane polyubiquitin nucleotide sequences. The sugarcane polyubiquitin nucleotide sequences are useful in regulating expression of a nucleic acid sequence of interest in monocotyledonous and dicotyledonous plants.

Abstract: The invention provides purified proteins, DNA sequences that code on expression therefore, and recombinant nucleic acid sequences, including hosts transformed with such sequences, for transforming coffee plants to reduce or eliminate the synthesis of caffeine. Coffee plant cells are transformed by means of transforming vectors that comprise a nucleic acid sequence, operably linked to a transcription promoter in an antisense or sense orientation, that codes on transcription for an RNA that has a length sufficient to interfere with the expression of a specific enzyme in the pathway for caffeine biosynthesis. Coffee plants regenerated from transformed coffee plant cells exhibit caffeine production that has been reduced by up to 98% compared with untransformed coffee plants.

Abstract: Animals are produced following injection of adult somatic cell nuclei into enucleated oocytes. The invention provides a method for cloning an animal by directly inserting at least a portion of the adult somatic nucleus (including the minimum chromosomal material able to support development) into a recipient enucleated oocyte. Preferably, the nucleus is inserted by microinjection and, more preferably, by piezo electrically-actuated microinjection. The oocyte is activated prior to, during, or up to about 6 hours after insertion of the nucleus, by electroactivation or exposure to a chemical activating agent, such as Sr2+. The activated renucleated oocyte is allowed to develop into an embryo and is transplanted to a host surrogate mother to develop into a live offspring.

Abstract: The present invention is a correlator for use in spread spectrum applications which utilizing continuous-time analog domain signal processing. The correlator include a multiplier which is coupled to an integration capacitance, and an integration reset circuit which is coupled to the integration capacitance. The correlator is designed to receive a first input signal and a second input signal. The multiplier multiplies the first input signal and the second input signal to produce a multiplier output current. The multiplier output current is then integrated by the integration capacitance which produces a correlator output voltage. The integration reset circuit then reset the integration capacitance to a reset voltage.

Abstract: This invention provides a process for preparing diastereomerically pure cryptophycin compounds.

Abstract: What is described is a recombinant poxvirus, such as vaccinia virus, containing foreign DNA from Plasmodium Merozoite Surface Antigen 1. What is also described is a vaccine containing the recombinant poxvirus for inducing an immunological response in a host animal inoculated with the vaccine.

Abstract: This invention discloses an efficient process for selectively sulfonating the primary alcohol of a diol containing both primary and secondary alcohols.

Abstract: A lysine-rich protein gene has been cloned from winged bean, Psophocarpus tetragonolobus, and its DNA sequence and deduced protein sequence are provided. Methods for transforming plant cells are provided that mature into plants having elevated levels of lysine in their seeds.

Abstract: A disinfestation treatment chamber for treating fruit, flower and vegetable commodities subject to infestation by quarantine pests. The commodities are disinfested so as to meet quarantine restrictions without adversely affecting the quality of the commodity. The commodity is heated inside of the chamber in hot air having a relative humidity of 30-85% until the temperature of the commodity exceeds the thermal death point temperature of the target pest but is not so high as to adversely affect the quality of the commodity. The temperature of the commodity is held at this temperature until the pest is killed. The commodity is loaded into the treatment chamber in bins onto a low friction conveyor track system which transports the bins from one end of the chamber to the other. The commodities are treated while they are inside of the treatment chamber. After treatment of the commodities, the bins are unloaded using the low friction conveyor track system out of the opposite end of the treatment chamber.

Abstract: A disinfestation treatment chamber for treating fruit, flower and vegetable commodities subject to infestation by quarantine pests. The commodities are disinfested so as to meet quarantine restrictions without adversely affecting the quality of the commodity. The commodity is loaded into the treatment chamber in bins onto a low friction conveyor track system which transports the bins from one end of the chamber to the other. The commodities are treated while they are inside of the treatment chamber.

Abstract: The invention provides a method for obtaining a live offspring having maternal chromosomes derived from a live first polar body of an oocyte that has completed the first meiotic division. It has been discovered herein that chromosomes in the first polar body are able to participate in normal embryonic development if they are allowed to complete the second meiotic division within an enucleated mature oocyte, and are then allowed to mingle with chromosomes of a spermatozoon. The invention further provides a method for producing up to four embyros or live offspring having the chromosomes of a single oocyte, by using both the first polar body chromosomes, and the second polar body chromosomes to reconstitute recipient enucleated (fertilized) oocytes.

Abstract: The present invention relates to a new and distinct variety of Colocasia esculenta (popularly known as taro) named ‘Pauakea’. ‘Pauakea’ differs distinctively from other taro plants by its unique combination of resistance to taro leaf blight caused by Phytophthora colocasiae, resistance to root rot caused by Pythium spp., vigorous growth, large mother corm size, and white corm of very good flour quality and good eating quality.

Abstract: The present invention relates to a new and distinct variety of Colocasia esculenta (popularly known as taro) named ‘Pa'lehua.’ ‘Pa'lehua’ differs distinctively from other taro plants by its unique combination of resistance to taro leaf blight caused by Phytophthora colocasiae, tolerance to root rot caused by Pythium spp., vigorous growth, large mother corm size, and purple corm of very good poi and eating qualities.

Abstract: The present invention relates to a new and distinct variety of Colocasia esculenta (popularly known as taro) named ‘Pa'kala’. ‘Pa'akala’ differs distinctively from other plants by its unique combination of resistance to taro leaf blight caused by Phytophthora colocasiae, high tolerance to root rot caused by Pythium ssp., vigorous growth, extra-large mother corm size, and pinkish corm of very good flour quality and good poi and eating qualities.