Patents Assigned to UNIVERSITY OF HONG KIONG
  • Publication number: 20130004980
    Abstract: A regulatable gene expression construct comprising a nucleic acid molecule comprising a two-way riboswitch operably linked to a target sequence. Also provided is a library screening strategy for efficient creation of target-specific riboswitches. A theophylline-repressible and IPTG-inducible riboswitch device achieves portable control of gene expression control in a ‘two-way’ manner. The default state of target genes is ON; the targets are switched off by adding theophylline, and switched back to the ON-state by adding IPTG without changing growth medium. The riboswitch device regulates gene expression in a portable, adjustable, and two-way manner with a variety of scientific and biotechnological applications.
    Type: Application
    Filed: June 27, 2012
    Publication date: January 3, 2013
    Applicant: UNIVERSITY OF HONG KIONG
    Inventors: Jian-Dong Huang, Ye Jin