Patents Assigned to University of Maryland Systems
  • HIV transgenic animals and uses therefor
    Patent number: 6156952
    Abstract: The invention provides transgenic animals comprising a lentiviral transgene, such as an HIV transgene. Also within the scope of the invention are cells and eggs from the transgenic animal. Further included are methods for identifying therapeutic compounds for preventing lentiviral infection and treating associated disease (e.g. AIDS).
    Type: Grant
    Filed: April 9, 1998
    Date of Patent: December 5, 2000
    Assignee: Constituent Institution of the University of Maryland System
    Inventors: Joseph L. Bryant, William C. Reid, Harry G. Davis, Jr.
  • Vibrio cholerae 01 (CVD111) and non-01 (CVD112 and CVD112RM) serogroup vaccine strains, methods of making same and products thereof
    Patent number: 5882653
    Abstract: Avirulent Vibrio cholerae strains of O1 (CVD111) and non-O1 (CVD112 and CVD112RM) serogroups having the DNA of the cholera toxin core and the RS1 sequences of the cholera toxin locus deleted, and further having a DNA encoding a resistance to mercury, and a DNA encoding the cholera toxin B subunit, or a part thereof sufficient to confer immunogenicity, re-inserted in the chromosome. Methods of making the avirulent V. cholerae O1 and non-O1 strains of the invention, and cholera vaccines using these strains.
    Type: Grant
    Filed: July 29, 1996
    Date of Patent: March 16, 1999
    Assignee: The University of Maryland System
    Inventors: James B. Kaper, Myron M. Levine
  • Vibrio cholerae strain CVD103Hg.sup.r, method of making same, and vaccines derived therefrom
    Patent number: 5399494
    Abstract: Method of isolating deletion mutants of Vibrio cholerae, wherein the deletion is predetermined by digestion with restriction endonucleases of known specificity. The deletions are inserted into the Vibrio cholerae chromosome by in vivo recombination between a plasmid carrying the desired deletion, with adjacent flanking sequences, and the Vibrio cholerae chromosome. The invention includes the isolation and characterization of a new Vibrio cholerae strain, (ATCC No. 55456), having a deletion in the tox gene, as defined by Acc I, Xba I, Cla I and/or restriction endonuclease sites, and carrying a mercury resistance gene. The invention also includes vaccines for protecting against the symptoms of cholera as well as methods for achieving this protection.
    Type: Grant
    Filed: August 28, 1992
    Date of Patent: March 21, 1995
    Assignee: The University of Maryland System
    Inventors: James B. Kaper, Myron M. Levine
  • Fluorometry method and apparatus using a semiconductor laser diode as a light source
    Patent number: 5196709
    Abstract: A method and apparatus for detecting the change in phase angle and/or modulation of emitted fluorescence of a fluorophore excited by modulated light from a laser diode. The light is both monochromatic and coherent, and can contain harmonic frequency components. The laser diode can be used in frequency-domain or phase-modulation measurements of fluorescence, using either phase or modulation techniques. The present invention is an improvement of the prior use of a frequency-doubled, cavity-dumped, dye laser in fluorometers. The invention provides an inexpensive light excitation source that is small in size, easily manageable, allows for short measurement times, and has lower power requirements.
    Type: Grant
    Filed: May 3, 1991
    Date of Patent: March 23, 1993
    Assignee: University of Maryland Systems
    Inventors: Klaus W. Berndt, Ignacy Gryczynski, Joseph R. Lakowicz