Abstract: The present invention relates to compositions and methods useful for analyzing lariat RNA, which plays a role in the regulation of gene expression. A sample of RNA is specifically treated to remove linear mRNA and enrich for lariat RNA. The enriched lariat RNA sample may be analyzed further to identify introns, branch point sequences, alternative splicing patters, and gene transcription levels. The enriched lariat RNA sample may also be exploited as a detection or compound screening tool, as well as other uses.
Type:
Grant
Filed:
October 22, 2014
Date of Patent:
September 26, 2017
Assignee:
THE CURATORS OF THE UNIVERSITY OF MISSOURI UNIVERSITY OF MISSOURI, OFFICE OF IPA
Abstract: Disclosed are compositions comprising collagen covalently bound to particles, wherein covalent bonds are formed between reactive groups of the collagen and reactive groups of the particles, and wherein the particles have an average particle diameter ranging from 20 to 1000 nanometers. Also disclosed are various methods that utilize the compositions.
Type:
Application
Filed:
June 7, 2011
Publication date:
March 22, 2012
Applicant:
UNIVERSITY OF MISSOURI, OFFICE OF IPA
Inventors:
Anthony Harris, Jonathan Thompson, Rebecca Rone, Sheila Grant, David Grant