Abstract: A transgenic animal containing germ cells and somatic cells which contain a non-native recombinant gene which codes upon expression hexokinase capable of expressing hexokinase at significantly increased levels for study of the role of hexokinase in diabetes and other diseases. When these transgenic animals were exposed to a calmodulin stimulated model of diabetes increased levels of hexokinase activity were able to promote insulin secretion thereby reducing glucose levels in blood and transiently reduce the effects of diabetes.

Abstract: A process and apparatus for purifying DNA which has relatively high molecular weight from a biological sample containing DNA is described. The method comprises the steps (1) lysing the red blood cells in the sample; 2) lysing DNA-containing cells; (3) precipitating the proteins from sample; and (4) and rehydrating and recovering the DNA. method offers the advantages of performance in less than 15 minutes, not requiring organic or poisonous materials, compatibility with most anticoagulants, and requiring less solutions than conventional purification procedures, thus making it simpler and more economical to use.