Patents Assigned to University of North Texas Health Science Center at Fort
Worth
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Patent number: 8159676Abstract: The present invention includes methods for ratiometric detection of analytes by surface plasmon coupled emission detection that includes disposing a target on the metal layer of a surface plasmon resonance detection system; coupling a first analyte to a first fluorescent dye and a second analyte to a second fluorescent dye; contacting the first and second analytes to the target on the surface plasmon resonance detection system; and measuring the intensity of a first and a second surface plasmon resonance enhanced fluorescence emission ring, wherein the first and second rings, respectively, quantitatively represents the amount of first and second analyte within 50 nanometers of the metal surface.Type: GrantFiled: January 20, 2009Date of Patent: April 17, 2012Assignee: University of North Texas, Health Science Center at Fort WorthInventors: Zygmunt Gryczynski, Ignacy Gryczynski, Evgenia Matveeva, Julian Borejdo
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Patent number: 8119642Abstract: A family of structurally rigid dopamine D3 receptor selective ligands is described. The family of structurally rigid dopamine D3 receptor selective ligands has the formula wherein A is cis or trans —CH?CH—, —C?C—, or cyclohexyl. B is cis or trans —CH?CH— or absent. R1 represents an optionally substituted phenyl group, wherein said substituents are selected from the group consisting of: hydrogen, halogen, amino, nitro, hydroxyl, alkoxy, alkyl, acyl and pyridyl, and said substitution may occur at any of the ortho, meta, or para positions, or R1 represents a heteroaromatic ring. A preferred heteroaromatic ring is indole, quinoxoline, pyridyl, pyrimidyl, or imidazole. R2 and R3 may be independently hydrogen or a halogen, or R2 alone may be C1, C2, or C3 alkoxy, and m is 1 or 2, and n is 0, 1, or 2.Type: GrantFiled: September 14, 2009Date of Patent: February 21, 2012Assignees: The United States of America as represented by the Secretary of the Department of Health and Human Services, University of North Texas Health Science Center at Fort WorthInventors: Amy Newman, Peter Grundt, Robert R. Luedtke
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Publication number: 20120040914Abstract: A method of enhancing the effectiveness of a chemotherapeutic agent, such as Temozolomide (TMZ), or ionizing radiation against glioblastoma is described in the present invention. The method comprises targeting putative membrane androgen receptor (termed mAR) in glioma cells to enhance the cytotoxic effects of a chemotherapeutic agent or ionizing radiation while simultaneously affording protection to neighboring neurons.Type: ApplicationFiled: August 11, 2011Publication date: February 16, 2012Applicant: UNIVERSITY OF NORTH TEXAS HEALTH SCIENCE CENTER AT FORT WORTHInventors: MEHARVAN SINGH, Adrienne Badeaux, Niki Mirshams
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Publication number: 20110207614Abstract: The present invention provides partially fluorescent nanoparticles and methods of making and using the partially fluorescent nanoparticle having a nanoparticle with a matrix and a fluorescent dye dispersed in or about the matrix, wherein at least a portion of the fluorescent dye has been anisotropically bleached.Type: ApplicationFiled: February 17, 2011Publication date: August 25, 2011Applicant: University of North Texas Health Science Center at Fort WorthInventors: Zygmunt Gryczynski, Ignacy Gryczynski, Rafal Luchowski, Julian Borejdo
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Patent number: 7956989Abstract: The present invention includes a microscope and a method for using the microscope for single molecule with reduced photobleaching of a fluorophore (20) that includes a light translucent material (16); a metal layer (18) disposed on the light translucent material (16); a medium (15) disposed on the metal layer (18), the medium (15) having one or more fluorophores (20) capable of binding a target analyte (e.g.Type: GrantFiled: January 22, 2008Date of Patent: June 7, 2011Assignee: University of North Texas Health Science Center at Fort WorthInventors: Zygmunt Gryczynski, Ignacy Gryczynski, Nils Calander, Julian Borejdo
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Publication number: 20100290982Abstract: The present invention includes compositions and methods of making an activated polymeric nanoparticle for targeted drug delivery that includes a biocompatible polymer and an amphiphilic stabilizing agent non-covalently associated with a spacer compound that includes at least one electrophile that selectively reacts with any nucleophilic on a targeting agent and places the targeting agent on the exterior surface of a biodegradable nanoparticle, wherein an active agent is encapsulated in or about the nanoparticle.Type: ApplicationFiled: April 23, 2010Publication date: November 18, 2010Applicant: University of North Texas Health Science Center at Fort WorthInventors: Amalendu Prakash Ranjan, Anindita Mukerjee, Jamboor K. Vishwanatha
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Publication number: 20090269281Abstract: The present invention includes compositions and methods for treating a patient with cardiac hypertrophy by providing the patient with an effective amount of a Calmodulin kinase kinase inhibitor that is sufficient to treat cardiac hypertrophy.Type: ApplicationFiled: September 9, 2008Publication date: October 29, 2009Applicant: University of North Texas Health Science Center at Fort WorthInventors: Stephen R. Grant, Thomas G. Valencia
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Patent number: 7605259Abstract: A family of structurally rigid dopamine D3 receptor selective ligands is described. The family of structurally rigid dopamine D3 receptor selective ligands has the formula wherein A is cis or trans —CH?CH—, —C?C—, or cyclohexyl. B is cis or trans —CH?CH— or absent. R1 represents an optionally substituted phenyl group, wherein said substituents are selected from the group consisting of: hydrogen, halogen, amino, nitro, hydroxyl, alkoxy, alkyl, acyl and pyridyl, and said substitution may occur at any of the ortho, meta, or para positions, or R1 represents a heteroaromatic ring. A preferred heteroaromatic ring is indole, quinoxoline, pyridyl, pyrimidyl, or imidazole. R2 and R3 may be independently hydrogen or a halogen, or R2 alone may be C1, C2, or C3 alkoxy, and m is 1 or 2, and n is 0, 1, or 2.Type: GrantFiled: September 15, 2003Date of Patent: October 20, 2009Assignee: University of North Texas Health Science Center at Fort WorthInventors: Amy Newman, Peter Grundt, Robert R. Luedtke
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Publication number: 20090218516Abstract: The present invention includes methods for ratiometric detection of analytes by surface plasmon coupled emission detection that includes disposing a target on the metal layer of a surface plasmon resonance detection system; coupling a first analyte to a first fluorescent dye and a second analyte to a second fluorescent dye; contacting the first and second analytes to the target on the surface plasmon resonance detection system; and measuring the intensity of a first and a second surface plasmon resonance enhanced fluorescence emission ring, wherein the first and second rings, respectively, quantitatively represents the amount of first and second analyte within 50 nanometers of the metal surface.Type: ApplicationFiled: January 20, 2009Publication date: September 3, 2009Applicant: University of North Texas Health Science Center at Fort WorthInventors: Zygmunt Gryczynski, Ignacy Gryczynski, Evgenia Matveeva, Julian Borejdo
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Publication number: 20090156637Abstract: The present invention includes compositions and methods for the protection of one or more central nervous system cells from trauma, when administered before, during or after the trauma, wherein the composition includes an effective amount of a butyrophenone, e.g., a 1-linked phenyl butyrophenone that is electronegative along the butyl chain and/or a Sigma-1 receptor antagonist.Type: ApplicationFiled: February 23, 2009Publication date: June 18, 2009Applicant: University of North Texas Health Science Center of Fort WorthInventors: John A. Schetz, James Simpkins, Jeffery Talbert
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Patent number: 7524622Abstract: Natural killer (NK) cells possess the inherent capacity to kill various tumor and virally infected cells. A large family of NK cell receptors belongs to the C-type lectin super-family. Genes in the NK gene complex encode type II receptors and examples include the families of NKR-P1, Ly-49, and NKG2 receptors. Examples of other C-type lectin-like receptors that occur as individual genes are CD94, CD69 and AICL. The invention includes a cDNA that encodes a predicted protein of 191 amino acid residues having similarity to the carbohydrate recognition domain of C-type lectins. The predicted protein of LLT1 shows 59 and 56% similarity to AICL and CD69, respectively. A monoclonal antibody (L9.7) against LLT1 receptor was generated. Binding of mAb L9.7 to surface LLT1 induced interferon gamma production in YT, a human NK cell line, as well as in resting and IL-2 activated NK cells, without modulating cytotoxicity.Type: GrantFiled: April 14, 2005Date of Patent: April 28, 2009Assignee: University of North Texas Health Science Center at Fort WorthInventors: Porunelloor A. Mathew, Kent S. Boles
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Publication number: 20090075888Abstract: The present invention includes compositions and methods for screening for a candidate substance with ryanodine receptor (RyR)-modulatory activity, the method including: determining the ion-conducting ability and ability to change the concentration of the free cytoplasmic intracellular Ca2+ by the RyR modulated by Estrogen receptor-? (ER?) in cells or cell membranes expressing RyR and ER? combination with, or in the absence of the estrogen; contacting the cells or cell membranes with a candidate substance capable of modulation the interaction between RyR and ER?; and measuring the RyR mediated ion-conducting ability of the cells or cell membranes to change the concentration of the free cytoplasmic intracellular Ca2+ by the candidate substance, whereby the modulatory activity of the candidate substance on RyR/ER? interaction is determined.Type: ApplicationFiled: September 6, 2008Publication date: March 19, 2009Applicant: University of North Texas Health Science Center at Fort WorthInventors: Peter Koulen, Volodymyr Rybalchenko
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Publication number: 20080253961Abstract: The present invention includes compositions and methods of making an activated polymeric nanoparticle for targeted drug delivery that includes a biocompatible polymer and an amphiphilic stabilizing agent non-covalently associated with a spacer compound that includes at least one electrophile that selectively reacts with any nucleophilic on a targeting agent and places the targeting agent on the exterior surface of a biodegradable nanoshell, wherein an active agent is loaded with the nanoshell.Type: ApplicationFiled: April 11, 2008Publication date: October 16, 2008Applicant: University of North Texas Health Science Center at Fort WorthInventors: Arthur R.C. Braden, Jamboor K. Vishwanatha, Erica Kafka
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Publication number: 20080231834Abstract: The present invention includes a microscope and a method for using the microscope for single molecule with reduced photobleaching of a fluorophore (20) that includes a light translucent material (16); a metal layer (18) disposed on the light translucent material (16); a medium (15) disposed on the metal layer (18), the medium (15) having one or more fluorophores (20) capable of binding a target analyte (e.g.Type: ApplicationFiled: January 22, 2008Publication date: September 25, 2008Applicant: University of North Texas Health Science Center at Fort WorthInventors: Zygmunt Gryczynski, Ignacy Gryczynski, Nils Calander, Julian Borejdo
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Publication number: 20080193915Abstract: The subject invention provides novel isotope-labeled dinitrophenylhydrazines (DNPHs) and methods for their use in detecting and/or quantifying carbonyl groups in proteins and other analytes. In particular, the present invention provides novel methods for identifying biomarkers of oxidative stress, which can be used to either forecast or detect diseases and/or conditions associated with oxidative stress. In one embodiment of the invention, isotope-labeled DNPHs are derived from [13C6]chlorobenzene.Type: ApplicationFiled: September 29, 2005Publication date: August 14, 2008Applicants: UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC., UNIVERSITY OF NORTH TEXAS HEALTH SCIENCE CENTER AT FORT WORTHInventors: Laszlo Prokai, Michael J. Forster
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Publication number: 20080085882Abstract: The present invention includes compositions and method to improve the therapeutic index of anti-cancer agents using a novel anti-cancer agent and a modulator or potentiator thereof.Type: ApplicationFiled: August 17, 2007Publication date: April 10, 2008Applicant: University of North Texas Health Science Center at Fort WorthInventors: Wolfram Siede, Rouel Roque
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Publication number: 20080045419Abstract: The present invention includes a system and methods for the detection, isolation and characterization of candidate agents in a functional screening assay in agarose.Type: ApplicationFiled: August 17, 2007Publication date: February 21, 2008Applicant: University of North Texas Health Science Center at Fort WorthInventor: Wolfram Siede
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Patent number: 7300926Abstract: The present invention relates to novel estrogen-related steroidal quinols and their use as drugs for estrogen replacement therapy. The quinols of the present invention provide improved physicochemical properties, increased bioavailability, and improved distribution into tissues, bone, in the cardiovascular system, and in the CNS (central nervous system) with only a slight estrogenic action or no estrogenic action in the uterus. The compounds are suitable for the production of pharmaceutical agents for use in numerous indications (for example, estrogen replacement therapy, prevention and treatment of osteoporosis).Type: GrantFiled: September 17, 2003Date of Patent: November 27, 2007Assignees: University of Florida Research Foundation, Inc., University of North Texas Health Science Center at Fort WorthInventors: Laszlo Prokai, Katalin Prokai, James Simpkins
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Publication number: 20070141581Abstract: The present invention includes compositions, kits and methods for specifically and differentially activating a membrane androgen receptor and their use for comparing the binding specificity of one or more drugs to a membrane androgen receptor and to an intracellular androgen receptor, wherein a difference in drug binding is indicative of differential receptor binding and may be used to diagnose and treat diseases and conditions associated with androgens.Type: ApplicationFiled: December 15, 2005Publication date: June 21, 2007Applicant: University of North Texas Health Science Center at Fort WorthInventors: Meharvan Singh, Joshua Gatson
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Publication number: 20070129611Abstract: The present invention provides a system, method and apparatus for assessing a person's menopausal or post-hysterectomy symptoms by providing a first set and a second set of questions to the person, receiving answers to the first set and second set of questions and assessing the person's symptoms based on the received answers. The first set of questions includes four or more questions directed to one or more physical conditions. The second set of questions includes two or more questions directed to one or more medical conditions. The physical conditions may include a duration of the symptoms, a frequency of the symptoms, an episode duration of the symptoms, an intensity of the symptoms, a bothersomeness of the symptoms and an onset age of the symptoms. The medical conditions may include a hysterectomy, a hormone therapy, a non-hormonal therapy and a contraception type.Type: ApplicationFiled: December 6, 2006Publication date: June 7, 2007Applicant: University of North Texas Health Science Center at Fort WorthInventors: Anna Ratka, James Simpkins