Abstract: The present invention includes nucleic acid hybrid molecules capable of entering cells comprising at least one vivo-morpholino oligonucleotide (vivo-MO) comprising a guanidine-rich head conjugated to the 5? end, and at least one standard oligonucleotide comprising a gene-specific sequence and a standard oligonucleotide pairing sequence, wherein the standard oligonucleotide is bound to the vivo-morpholino oligonucleotide through base pairing to form a hybrid and wherein the vivo-morpholino oligonucleotide pairing sequence is complementary to the standard oligonucleotide pairing sequence.

Abstract: A coupled nanomanipulation and nanospray mass spectrometry (NMS) system for single cell, single organelle, and ultra-trace molecular analysis is disclosed herein. The system primarily comprises a bio-workstation coupled to a NMS. The bio-workstation primarily comprises of a nanomanipulator stage with a plurality of nano-positioners attached to a cabinet with a piezo voltage source and a pressure injector. The present invention further describes a fingerprint lift method that when coupled with the system disclosed herein can be used for retrieval and analysis of trace amounts of drug and explosive residues. The system described herein has been used in the areas of trace and document analysis within the forensic field, trace fiber analysis, and electrostatic lifts for illicit drugs, as well as document and painting analysis.

Abstract: An apparatus and method for nonlinear conversion of laser light is described herein. The apparatus of the instant invention comprises a fiber coupled light source, a cavity in optical alignment with the first light beam from the light source, wherein the cavity comprises: two concave mirrors, one or more Brewster-cut periodically poled crystals, and a cavity servo to lock the length of cavity to laser frequency. In one aspect of the present invention the feedback to the lock is S-polarized component of first laser light reflected off the Brewster surface of nonlinear crystal.

Abstract: The present invention provides system, method and apparatus for energizing one or more brake lights on a vehicle without an operator of the vehicle applying a brake of the vehicle by determining an acceleration of the vehicle, energizing the brake lights on the vehicle whenever the acceleration of the vehicle equals or exceeds a braking threshold, and deenergizing the brake lights on the vehicle whenever: (a) the brake lights are energized, (b) the acceleration of the vehicle is less than the braking threshold, (c) a specified period of time has elapsed since the brake lights were energized, and (d) the operator is not applying the brake of the vehicle.

Abstract: The present invention provides a method and a system for automatic keyword extraction based on supervised or unsupervised machine learning techniques. Novel linguistically-motivated machine learning features are introduced, including discourse comprehension features based on construction integration theory, numeric features making use of syntactic part-of-speech patterns, and probabilistic features based on analysis of online encyclopedia annotations. The improved keyword extraction methods are combined with word sense disambiguation into a system for automatically generating annotations to enrich text with links to encyclopedic knowledge.