Abstract: The present invention relates to methods for preserving cells, in particular primary cells such as hepatocytes. In particular, the present invention relates to preserving cells within a gel comprising a hydrolysed gelatin and culturing the cells at a temperature between 0 and 15° C.
Type:
Application
Filed:
September 5, 2008
Publication date:
February 12, 2009
Applicants:
University of Wales, Cardiff, ABCELLUTE Limited
Inventors:
Benjamin J. N. Griffiths, Peter John Evans
Abstract: A method of determining the presence or onset of capillary leakage includes introducing into the blood stream of the subject a first low dosage of a low molecular weight macromolecular material and determining the volume of distribution thereof (an apparent volume of distribution). The determined value of volume of distribution is influenced not only by extravasation through the capillary wall but also by binding of smaller molecules to pores or receptor binding sites in the wall which occurs much faster than extravasation and is an important indicator of the onset of capillary leakage. The process is repeated with a high dose of high molecular weight macromolecular material to determine a further volume of distribution (the true circulating plasma volume). The two values are compared to determine the presence or onset of capillary leakage.
Type:
Grant
Filed:
October 15, 1999
Date of Patent:
March 12, 2002
Assignees:
University Wales College of Medicine, University of Wales, Cardiff
Inventors:
John Gareth Jones, Frederick Stephen Wusteman, Charles Alexander James Wardrop