Abstract: Flow cytometry concepts are modified to enable dynamic characterizations of particles to be obtained using optical scattering data. Particles in flow will be introduced into a sample volume. Light scattered by a particle in the sample volume is collected and analyzed. What differentiates the concepts disclosed herein from conventional flow cytometry is the use of an acoustic source that is disposed to direct acoustic energy into the sample volume. As the particle passes through the sample volume, it responds to the acoustic energy, causing changes in the light scattered by the particle. Those changes, which arc not measured during conventional flow cytometry, can be analyzed to determine additional physical properties of the particle.
Type:
Application
Filed:
June 20, 2012
Publication date:
January 3, 2013
Applicant:
University of Washington, UW Tech Transfer, Invention Licensing
Abstract: Embodiments in accordance with the present invention relate to methods and apparatuses for concentrating and isolating Circulating Tumor Cells (CTCs) from body fluids. One embodiment of the present invention includes a micro-fabricated or nano-fabricated device having channels configured for separating and excluding. Embodiments in accordance with the present invention utilize features that reduce the hydrodynamic pressure experienced by the cells during the separation, isolation and concentration processes, and therefore reduce the likelihood of cell lysis or other damage to the cells.
Type:
Application
Filed:
June 20, 2007
Publication date:
October 9, 2008
Applicant:
University of Washington, UW Tech Transfer - Invention Licensing
Abstract: The present invention provides methods and compositions for amplifying the detection signal in surface plasmon resonance (SPR)-based flow systems. The signal amplification methods comprise the use of well established marker systems that provide a precipitate. The marker systems include, for example, enzyme and nucleation systems. Enzymes suitable for use as a marker system include peroxidases and phosphatases. The amplification system is useful in any SPR-based detection system including microfluidic systems, e.g., “lab on a chip” systems and the like. The methods can comprise any SPR-based assay format, including typical immunoassay formats. The immunoassay formats can include competitive and sandwich assays. Analyte capture agents can include antibodies, lectins, carbohydrates, polynucleotides, receptor proteins, and the like.
Type:
Grant
Filed:
December 13, 2005
Date of Patent:
July 29, 2008
Assignee:
University of Washington UW Tech Transfer - Invention Licensing
Inventors:
Melissa Hasenbank, Paul Yager, Elain Fu, Kjell Nelson
Abstract: Methods and system for counterbalancing accelerations and/or torques caused by actuation of an actuation element and a scanning element. A scanning beam device may comprise a counterbalance to generate forces and/or torques that are substantially equal and substantially opposite to the forces and/or torque generated by the scanning of the actuation element.
Type:
Application
Filed:
July 21, 2005
Publication date:
January 25, 2007
Applicant:
University of Washington UW Tech Transfer - Invention Licensing
Abstract: Adenovirus fiber mutated in the regions involved in the recognition and the binding of blood factor proteins, and adenoviruses comprising such fibers are provided.
Type:
Application
Filed:
May 3, 2004
Publication date:
December 14, 2006
Applicant:
UNIVERSITY OF WASHINGTON UW TECH TRANSFER- INVENTION LICENSING