Abstract: A method and process for the treatment of wastewater containing phosphorous and nitrogen. The wastewater is first anaerobically treated to produce an anaerobic effluent from which insoluble organic carbon is separated to form a sludge rich in organic carbon that is used as a substrate during anoxic treatment of the wastewater by de-nitrifying phosphorous accumulating organisms (DPAO's) and ordinary de-nitrifying organisms. The separation of insoluble organic carbon is normally conducted using a clarifier located intermediate the anaerobic and anoxic bio-reactors. In one embodiment, the ammonia rich clarifier supernatant is directed to an aerobic reactor for nitrification and the nitrate produced is recycled to the anoxic bio-reactor. The final effluent may be membrane filtered to retain nitrifying biomass within the aerobic bio-reactor. The invention reduces overall hydraulic residence time and sludge volume, which results in a smaller, less expensive wastewater treatment system.