Abstract: This invention describes a method for identifying bacteria. In particular, this invention relates to a method for identifying and quantifying mycobacteria from a sputum sample taken from a subject using flow cytometry. Further described is the use of flow cytometry to identify and quantify Mycobacterium tuberculosis from sputum-derived samples. Once identified and quantified, the samples are spotted onto filter cards for use in verification of an existing method of diagnosis, calibration of an existing method of diagnosis and/or the establishment of an external quality control system for use in conjunction with these methods of diagnosis. In one embodiment the method is used to diagnose tuberculosis (TB).

Abstract: A DNA polynucleotide is described herein having a modified sequence of a target gene, wherein any one type of nucleotide in the target gene sequence has been chemically modified to another type of nucleotide; and a complementary sequence of the unmodified target gene; wherein either one of the modified sequence or the complementary sequence is in a reverse orientation to the other sequence; and wherein the RNA sequence transcribed from the DNA polynucleotide forms a duplex between the modified sequence and the complementary sequence so that a long double stranded RNA (IdsRNA) duplex forms between the modified and complementary sequences with base pair mismatches where the nucleotides have been modified, the IdsRNA duplex being capable of inhibiting expression of the target gene. RNA polynucleotides and IdsRNA duplexes transcribed by the DNA polynucleotide are also described, as is a method for producing the IdsRNA duplexes. These IdsRNA duplexes can be used in gene silencing.

Abstract: A spark gap protection device is provided. The device comprises a plurality of spark gaps and a plurality of windings. Each spark gap is connected to at least one winding which is inductively coupled to an associated winding connected to another spark gap so that, in use, a surge current flowing across a spark gap and through a winding connected to the spark gap induces a voltage in the associated inductively coupled winding connected to another spark gap. This forces the other spark gap to trigger, thereby distributing the surge current between the spark gaps.

Abstract: A spark gap protection device is provided. The device comprises a plurality of spark gaps and a plurality of windings. Each spark gap is connected to at least one winding which is inductively coupled to an associated winding connected to another spark gap so that, in use, a surge current flowing across a spark gap and through a winding connected to the spark gap induces a voltage in the associated inductively coupled winding connected to another spark gap. This forces the other spark gap to trigger, thereby distributing the surge current between the spark gaps.