Abstract: Methods, compositions, and systems for detecting gamma radiation is disclosed and described. A compound for detecting gamma radiation can comprise a conjugated imidazole having the following structure: [Formula I] where at least one of R1, R2, and R3 are conjugated organic groups. Additionally, the conjugated imidazole can be capable of reacting with a radical or ion formed by the reaction of gamma radiation with a radical generating component such as a halogen solvent to decrease a molar extinction coefficient of the conjugated imidazole in the visible light region or to quench fluorescence of the conjugated imidazole. As a sensor (100), a radiation detection indicator (108) can indicate the change in molar extinction coefficient or fluorescence of the conjugated imidazole material (120) upon exposure to gamma radiation.

Abstract: Methods, devices, and kits are provided for performing PCR in <20 seconds cycle, with improved efficiency and yield.

Abstract: Disclosed are compositions and methods for regulating eosinophils.

Abstract: A biomolecular assay includes a substrate with a metallic layer on at least one surface thereof. The metallic film includes nanocavities. The nanocavities are configured to enhance signals that are representative of the presence or amount of one or more analytes in a sample or sample solution, and may be configured to enhance the signal by a factor of about two or more or by a factor of about three or more. Such signal enhancement may be achieved with nanocavities that are organized in an array, randomly positioned nanocavities, or nanocavities that are surrounded by increased surface area features, such as corrugation or patterning, or nanocavities that have quadrilateral or triangular shapes with tailored edge lengths, or with a plurality of nanoparticles. Methods for fabricating biomolecular substrates and assay techniques in which such biomolecular substrates are used are also disclosed.

Abstract: The invention provides methods, compositions, and devices for promoting adhesion or migration of endothelial cells.

Abstract: Described herein are compositions useful in anticancer treatment and prevention. The compositions are composed of (a) an O2-aryl substituted diazeniumdiolate and (b) an amphiphile, wherein the amount of amphiphile is sufficient to produce a liposome or micelle. The compositions described herein provide numerous advantages such as increased solubility and stability of the O2-aryl substituted diazeniumdiolate in vivo. The compositions also do not induce hypotension. Methods for using the compositions in anticancer treatment and prevention are also described herein.

Abstract: Methods, systems, and compounds for detecting modified nucleic acid bases are disclosed and described. The methods provide for detecting a nucleic acid lesion and can include directing a nucleic acid adduct into a channel, wherein the nucleic acid adduct includes a nucleic acid having a lesion and a current modulating compound coupled to the nucleic acid at the lesion (110), and measuring a change in current through the channel in response to the current modulating compound to detect the lesion (112). The method can optionally include forming the nucleic acid adduct. Also provided is a method for identifying the number of repeat nucleotides in at least a portion of a nucleic acid strand, a method of assigning a registration marker within a nucleic acid, and a method of obtaining sequence information from a nucleic acid comprising assigning a registration marker on the nucleic acid.

Abstract: Disclosed is a spotter device and methods for the formation of microassays, biochips, biosensors, and cell cultures. The spotter may be used to deposit highly concentrated spots of protein or other materials on a microarray slide, wafer, or other surface. It may also be used to perform various chemistry steps on the same spots. The spotter increases the surface density of substances at each spot by directing a flow the desired substance (or a solution thereof) over the spot area until surface saturation is accomplished. The spotter may be loaded by well plate handling equipment. The spotter uses wells, microfluidic conduits, and orifices to deposit proteins, other biomolecules, or chemicals on a spot on, a separate surface. Each orifice is connected to two wells via microconduits. When the spotter contacts a surface, a seal is formed between the orifices and the surface. The same or different substances may be flowed across each orifice. Any number of orifices may be incorporated into a spotter.

Abstract: A system capable of providing directional cues to a user of the vehicle comprises a vehicle component, configured to be installed within a vehicle that is adapted to be controlled by a user. A tactile display system is operably coupled to the vehicle component. The tactile display system includes: a contact pad, operable to engage a target area of a user's skin; a restraining system, operable to restrain a portion of the user's skin distal from the target area of the skin; and an actuation system, operably coupled to one of: i) the contact pad and the ii) restraining system. The actuation system is capable of moving the contact pad and the restraining system relative to one another to apply a tangential displacement or shear force across the target area of the user's skin to thereby provide tactile information to the user.

Abstract: A system (100) and method for creating three dimensional images using probe molecules is disclosed and described. A sample is mounted on a stage (160). The sample has a plurality of probe molecules. The sample is illuminated with light, causing the probe molecules to luminesce. The probe luminescence can be split into at least four paths corresponding to at least four detection planes corresponding to object planes in the sample. The at least four detection planes are detected via a camera (155). Object planes in corresponding recorded regions of interest are recorded in the camera (155). A signal from the regions of interest is combined into a three dimensional image.

Abstract: In one aspect, the invention relates to substituted (E)-N?-(1-phenylethylidene)benzohydrazide analogs, derivatives thereof, and related compounds, which are useful as inhibitors of lysine-specific histone demethylase, including LSD1; synthetic methods for making the compounds; pharmaceutical compositions comprising the compounds; and methods of using the compounds and compositions to treat disorders associated with dysfunction of the LSD1. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention.

Abstract: The ability of a receiver to determine when a transmitter has changed location is important for energy conservation in wireless sensor networks, for physical security of radio tagged objects, and for wireless network security in detection of replication attacks. In embodiments of the present invention, a measured temporal link signature is determined to uniquely identify the link between a transmitter and a receiver. The temporal link signature is an impulse response of a transmitted signal from the transmitter over a channel between the transmitter and a receiver over a channel. When the transmitter changes location, or if an attacker at a different location assumes the identity of the transmitter, the proposed link distinction methodology reliably detects the change in the physical channel. This detection can be performed at a single receiver or collaboratively by multiple receivers.

Abstract: A method of reacting compounds can include directing a liquid into a helical constrained flow (37) having an inner circumferential flow surface and an outer circumferential flow surface. The helical constrained flow (37) can be formed around an axial interior volume (38). At least a portion of the helical constrained flow can be exposed to a sparging portion (35) to allow a fluid to be sparged into the liquid along the helical constrained flow (37). The fluid reactant can be sparged through the helical constrained flow so as to form a fluid product.

Abstract: Methods, assays, and products for the detection of small molecules are provided. In one aspect, for example, a method of detecting a small molecule in a sample can include reacting together a first half of a DNA split aptamer having a first reactive group coupled thereto, a second half of a DNA split aptamer having a second reactive group coupled thereto, where the DNA split aptamer is selective for the small molecule, and a sample containing the small molecule. The first half and the second half bind to the small molecule and the first reactive group and the second reactive group react to form an aptamer ligation product of the first half and the second half. The method can also include assaying for the aptamer ligation product in order to detect the small molecule presence in the sample.

Abstract: Disclosed are compositions and a method for amplification and detection of nucleic acid sequences based on continuous flow thermal gradient PCR.

Abstract: In one example embodiment, a method of volumetric image reconstruction of an examination region includes directing x-rays from an anode of an x-ray device towards the examination region from multiple positions relative to the examination region, including multiple focal spot positions radially shifted relative to the anode. X-rays that have passed through the examination region are detected and first multiple x-ray attenuation values are determined for each of the multiple positions. The first multiple x-ray values are based at least in part on the detected x-rays. Second multiple x-ray attenuation values associated with multiple levels are determined. The second multiple attenuation values are based at least in part on the first multiple attenuation values and the multiple positions. The method further includes generating a volumetric image reconstruction of the examination region based at least in part on the second multiple x-ray attenuation values.

Abstract: An apparatus for examining membrane-bound proteins in a cell can include a chamber with an insulating partition dividing the chamber into an upper well and a lower well, and a pore penetrating the insulating partition. The pore can have a size and shape so as to snugly hold a cell in place therein. The apparatus can further include circuitry for delivering a radio frequency signal to the cell. A belt electrode for delivering electrical signals to the cell can be located within the insulation partition and substantially encircling the pore. A measuring circuit for measuring cell membrane impedance to the radio frequency signal is also provided, and changes in the impedance can signal a change in state of a protein in the cell membrane.

Abstract: Integrated surgical systems and methods for using same are provided that can receive scanned images and produce a three dimensional model from the images. Based at least in pan on the three dimensional model, a processor generates code defining an optimized tool path, which is sent to a surgical machining system that can machine the desired portion of the patient. In one exemplary aspect, the integrated system operates in a clearly defined and pre-programmed manner with no necessity for in-situ sensory or guidance feedback.

Abstract: A method is provided for identifying documents that include a searchable form relevant to a topic. A document is received. If the received document comprises a form is determined. A form includes a field presented to a user requesting information from the user. If the received document is determined to comprise a form, a determination is made concerning whether or not the form is a searchable form. A searchable form returns non-trivial information to a requester in response to a submission of the form. If the form is determined to be a searchable form, a determination is made concerning whether or not the form is relevant to an identified topic. If the form is determined to be relevant to the identified topic, the document is identified as a searchable form relevant to the identified topic.

Abstract: Downhole deployable tools for measuring tracer concentrations are disclosed. According to one embodiment, an apparatus comprises a support cable configured to allow the apparatus to be lowered into and raised from a wellbore. A housing is attached to the support cable. The housing includes a detector window on the exterior of the housing. A detector system within the housing includes a detector that measures tracer concentrations. The detector is operably connected to the detector window to direct energy or particles from the detector window to the detector.