Abstract: Methods of uniquely labeling or barcoding molecules within a cell, a plurality of cells, and/or a tissue are provided. Kits for uniquely labeling or barcoding molecules within a cell, a plurality of cells, and/or a tissue are also provided. The molecules to be labeled may include, but are not limited to, RNAs, cDNAs, DNAs, proteins, peptides, and/or antigens.

Abstract: Infectious diseases can be transmitted to humans, or between humans and animals, by airborne viruses and bacteria, known as infectious aerosols. Current protective measures that individuals can take to avoid inhaling such aerosols are either marginally effective (personal face masks) or impractical (self-contained breathing apparatuses). Building ventilation systems employing high-efficiency filters to prevent distribution of such aerosols suffer from high energy costs and high filter replacement costs. The development of conventional, intramuscularly administered vaccines takes months or years to produce enough doses to protect a population from a rapidly spreading infectious disease. Airborne viruses and bacteria have been shown to be completely inactivated when exposed to non-thermal plasmas.

Abstract: Covalently cross-linked pilus polymers displayed on the cell surface of Gram-positive bacteria are assembled by class C sortase enzymes. These pilus-specific transpeptidases located on the bacterial membrane catalyze a two-step protein ligation reaction—first, cleaving the LPXTG motif of one pilin protomer to form an acyl-enzyme intermediate, and second, joining the terminal threonine to the nucleophilic lysine residue residing within the pilin motif of another pilin protomer. Informed by the high-resolution crystal structures of corynebacterial pilus-specific sortase (SrtA) and by developing structural variants of the sortase enzyme whose catalytic pocket has been unmasked by activating mutations, we have developed new reagents capable of forming isopeptide bonds in vitro. The reagents disclosed herein can catalyze ligation of isolated SpaA domains in vitro provide a facile and versatile new platform for protein engineering and bio-conjugation that has major implications for biotechnology.

Abstract: A low modulus corrosion-resistant alloy is disclosed, and comprises five principal elements, wherein the five principal elements are Zr, Nb, Ti, Mo, and Sn. Experimental data reveal that, samples of the low modulus corrosion-resistant alloy all include following characteristics: hardness of at least 250 HV, Young's modulus less than 100 GPa, yield strength greater than 600 MPa, and critical pitting potential greater than 1.3V. As a result, experimental data have proved that this low modulus corrosion-resistant alloy has a significant potential for application in the manufacture of biomedical articles including medical devices and surgical implants. In addition, this low modulus corrosion-resistant alloy is also suitable for application in the manufacture of various industrially-producible articles, including springs, coils, wires, clamps, fasteners, blades, valves, elastic sheets, spectacle frames, sports equipment, and other high-strength low-modulus corrosion-resistant structural materials.

Abstract: Provide herein are compounds with a general chemical structure of: Substituents R1 and R2 independently are H, Cl, F, Br, CH3, CF3, SH, —N(C1-3alkyl)2, —NHC(O)C1-3alkyl, or —NHC(O)C5-7cycloalkyl, substituent R3 is H or C1-3 alkyl and R4 is a bridged cycloalkene such as a bridged cyclohexene or a bridge-substituted cyclohexene. The compounds are therapeutics to treat a cancer, such as breast cancer, or metastatic cancers, to inhibit RUNX2 activity, such as protein expression, in a cancer cell and to increase survival of a subject with breast cancer.

Abstract: The invention relates to a process for treating food, in particular for curing meat, comprising adding to the food a compound of Formula III, which is a derivative of S-nitroso-cysteine. Use of the compound of Formula III as food preservative and compositions for curing meat are also provided by the invention.

Abstract: A video encoding method, a video decoding method, an apparatus, a device, and a storage medium include parsing a first flag from the video bitstream when a video bitstream is allowed to be decoded by referencing a library picture corresponding to a library picture bitstream, using a value of a target parameter of the video bitstream as a value of a target parameter of the library picture bitstream referenced by the video bitstream when a value of the first flag is a first value, and reconstructing, based on the value of the target parameter of the library picture bitstream referenced by the video bitstream and the library picture bitstream referenced by the video bitstream to obtain the library picture corresponding to the library picture bitstream referenced by the video bitstream.

Abstract: A method for fast extracting an organic pollutant in a complex system is disclosed, which includes following steps. A surface-enhanced Raman scattering (SERS) spectrum of an organic pollutant is divided to obtain P wavelength sub-intervals with overlapping regions. The P wavelength sub-intervals are screened to obtain ? wavelength sub-intervals. The ? wavelength sub-intervals are screened to obtain a required wavelength sub-interval. The required wavelength sub-interval is screened to obtain a required wavelength subset. A method and a system for fast detecting an organic pollutant in a complex system are also disclosed.

Abstract: In certain aspects, the present invention provides methods for inducing a stable gene modification of a target nucleic acid via homologous recombination in a primary cell, such as a primary blood cell and/or a primary mesenchymal cell. In certain other aspects, the present invention provides methods for enriching a population of genetically modified primary cells having targeted integration at a target nucleic acid. The methods of the present invention rely on the introduction of a DNA nuclease such as a Cas polypeptide and a homologous donor adeno-associated viral (AAV) vector into the primary cell to mediate targeted integration of the target nucleic acid. Also provided herein are methods for preventing or treating a disease in a subject in need thereof by administering to the subject any of the genetically modified primary cells or pharmaceutical compositions described herein to prevent the disease or ameliorate one or more symptoms of the disease.

Abstract: Provided herein are methods for inhibiting PCSK9, reducing PCSK9 levels, and for treating or preventing related conditions and disorders, in a subject, which includes administering to a subject an effective amount of a compound which includes derivatized cycloalkyl[b]indoles, e.g., cyclopenta-, cyclohexa- and cyclohepta[b]indoles.

Abstract: Next Generation DNA sequencing promises to revolutionize clinical medicine and basic research. However, while this technology has the capacity to generate hundreds of billions of nucleotides of DNA sequence in a single experiment, the error rate of approximately 1% results in hundreds of millions of sequencing mistakes. These scattered errors can be tolerated in some applications but become extremely problematic when “deep sequencing” genetically heterogeneous mixtures, such as tumors or mixed microbial populations. To overcome limitations in sequencing accuracy, a method Duplex Consensus Sequencing (DCS) is provided. This approach greatly reduces errors by independently tagging and sequencing each of the two strands of a DNA duplex. As the two strands are complementary, true mutations are found at the same position in both strands. In contrast, PCR or sequencing errors will result in errors in only one strand.

Abstract: The present invention discloses a method for preparation of vitamin and carotenoid powder; the vitamin and carotenoid powder comprises vitamin, carotenoid microcapsule and physical gel protection film covered on the surface of the vitamin and carotenoid microcapsule; the physical gel protection film is made from super-molecular system; the super-molecular system comprises the following constituents of parts in weight: vegetable oil: 6-30 parts; gel: 0.5-3 parts and antioxidant: 0.5-3 parts. The hot super-molecular solution is sprayed on the surface of cold vitamin and carotenoid microcapsule during preparation to form a specific 3D network structure that is used to bind the liquefied vegetable oil to form a physical gel protection film; the physical gel protection film has improved product storage stability as well as the its stability for application in feedstuff, food and health care products.

Abstract: Disclosed herein are systems and methods for determining metabolic pathway dynamics using time series multiomics data. In one example, after receiving time series multiomics data comprising time-series metabolomics data associated a metabolic pathway and time-series proteomics data associated with the metabolic pathway, derivatives of the time series multiomics data can be determined. A machine learning model, representing a metabolic pathway dynamics model, can be trained using the time series multiomics data and the derivatives of the time series multiomics data, wherein the metabolic pathway dynamics model relates the time-series metabolomics data and time-series proteomics data to the derivatives of the time series multiomics data. The method can include simulating a virtual strain of the organism using the metabolic pathway dynamics model.

Abstract: A database management system (DBMS) generates a query execution plan including information representing one or more database (DB) operations necessary for executing a query and executes the query based on the query execution plan. In the execution of the query, the DBMS dynamically generates a task for executing a DB operation and executes the dynamically generated task. The DBMS executes a task in a plurality of threads executed by a processor core.

Abstract: The present disclosure provides a stitching quality evaluation method and system, and a redundancy reduction method and system for low-altitude UAV remote sensing images, and belongs to the technical field of image processing. The method comprises: firstly acquiring ground images using a UAV under a preset overlap degree to obtain a low-altitude UAV remote sensing image set, then stitching the low-altitude UAV remote sensing image set to obtain a stitched image, and finally performing a quality evaluation on the stitched image using an improved BRISQUE algorithm to obtain an image quality score, which is applicable to quality evaluation of visible images and multispectral images at the same time through the improved BRISQUE algorithm. In addition, the present disclosure further provides an image redundancy reduction method based on the improved BRISQUE algorithm, thereby improving the image stitching efficiency and stitching quality.

Abstract: The invention relates to compositions of nicotinoyl ribosides and nicotinamide riboside derivatives and their methods of use. In some embodiments, the invention relates to methods of making nicotinoyl ribosides. In some embodiments, the invention relates to pharmaceutical compositions and nutritional supplements containing a nicotinoyl riboside. In further embodiments, the invention relates to methods of using nicotinoyl ribosides and nicotinamide riboside derivatives that promote the increase of intracellular levels of nicotinamide adenine dinucleotide (NAD+) in cells and tissues for improving cell and tissue survival.

Abstract: During training of deep neural networks, a Copernican loss (LC) is designed to augment the standard Softmax loss to explicitly minimize intra-class variation and simultaneously maximize inter-class variation. Copernican loss operates using the cosine distance and thereby affects angles leading to a cosine embedding, which removes the disconnect between training and testing.

Abstract: Disclosed is a link-based autonomous cell scheduling device including: a routing information manager that records and manages information on a node's preferred parent node and child nodes in a routing information table; a slot frame manager that generates and modifies a number of slot frames by referring to routing information in the routing information table, packet queue information, and transmission/reception result information; a slot frame schedule determiner that integrates a number of slot frames to generate one integrated slot frame corresponding to a global slot frame size and record the same in an integrated slot frame table, by referring to a link unicast slot frame table, a broadcast slot frame table, and an EB slot frame table; and a TSCH MAC layer driver that operates after checking for a cell assigned to the integrated slot frame by referring to the integrated slot frame table at the TSCH MAC layer.

Abstract: An embodiment of the present invention provides a method of creating a classifier indicative of a presence of a medical condition in a subject, comprising receiving chromatogram data indicative of a profile of volatile organic compounds in a sample from each of a first plurality of subjects having the medical condition and a second plurality of subjects without the medical condition, selecting one of the chromatogram data as reference chromatogram data, aligning the remaining chromatogram data in relation to the reference chromatogram data, extracting one or more features from the chromatogram data using a Mexican hat wavelet transform of one or more scales, selecting one or more features of the chromatogram data indicative of the medical condition, and constructing a classifier for determining a boundary between chromatogram data indicative of the medical condition and chromatogram data indicative of an absence of the medical condition.

Abstract: Provided herein are multi-functional particles. The particles may include poly(lactide-co-glycolide)-g-polyethylenimine (PLGA-g-PEI (PgP)), at least one targeting moiety, at least one therapeutic agent, and/or at least one nucleic acid. Also provided herein are methods of using the multi-functional particles.