Abstract: Methods are disclosed for rapid, reliable and simple isolation of RNA from formalin-fixed paraffin-embedded tissue samples. RNA purified in this manner can be used for quantitative measurement gene expression levels. The tissue sample can be a tumor or other pathological tissue.

Abstract: Methods and compositions for introducing a nucleic acid into the genome of at least one cell of a multicellular organism are provided. In the subject methods, a Sleeping Beauty transposon that includes the nucleic acid is administered to the multicellular organism along with a source of a Sleeping Beauty transposase activity. Administration of the transposon and transposase results in integration of the transposon, as well as the nucleic acid present therein, into the genome of at least one cell of the multicellular organism The subject methods find use in a variety of different applications, including the in vivo transfer of genes for use in, among other applications, gene therapy applications.

Abstract: A fast-tracking discrimination approach provides rapid acquisition useful for direct-sequence spread spectrum applications. In one example embodiment, the present invention is directed to a communication system receiver that operates a linearly normalizing discrimination process on a signal modulated by a waveform having a bit rate defined in terms of a bit-pulse duration (chip). For at least one reference code, correlation results are obtained from several timing offsets of the signal, where each timing offset is separated by a fixed chip offset. Using at least three consecutive ones of these correlation results, assuming that the correlation energy of band-limited signals is approximately quadratic, an accurate phase error is obtained for synchronizing the received signal.

Abstract: Disclosed herein is a sol-gel polymerization process for synthesizing metal-doped organic gels. The process polymerizes metal salts of hydroxylated benzenes or hydroxylated benzene derivatives with alkyl or aryl aldehydes to form metal-doped, wet, organic gels. The gels can then be dried by supercritical solvent extraction to form metal-doped aerogels or by evaporation to form metal-doped xerogels. The aerogels and xerogels can then be pyrolyzed.

Abstract: A quality assurance device is provided for ensuring the accuracy and reproducibility of the mechanical parameters of medical linear accelerators (Medical LINACs). The quality assurance devices is configured for placement within two parallel slots on the gantry of a Medical LINAC and includes off-the-shelf components for ensuring the accuracy and reproducibility of an optical distance indicator (ODI) distance measurement readout, collimator and gantry angles indicated by a display of the Medical LINAC, a radiation field size indicated by the Medical LINAC display, the centering of cross-hairs on the gantry with the intersection of the Medical LINAC axes, and alignment of the two lasers emanating from two positions toward the Medical LINAC with the intersection of the Medical LINAC axes.

Abstract: Methods and devices are disclosed for detecting vulnerable atherosclerotic plaque, or plaque at risk of reducing blood flow in a vessel, by identifying a region of elevated temperature along a living vessel wall. The disclosure that human atherosclerotic plaque with measurable temperature heterogeneity has the morphological characteristics of plaque that is likely to ulcerate provides a new and sensitive technique for detecting and treating these dangerous plaques before myocardial infarction and its consequences occur. The disclosed methods are advantageous over conventional plaque detection techniques because they are capable of differentiating between those plaques that are at great risk of rupture, fissure, or ulceration, and consequent thrombosis and occlusion of the artery, and those that are not presently at risk. Infrared heat-sensing catheters useful for identifying potentially fatal arterial plaques in patients with disease of the coronary or other arteries are also described.

Abstract: A computer-based system provides arm movement therapy for patients with sensory motor impairments. The system is based off a web site that provides access to assessment and therapy exercises designed to test and improve motor coordination in the arm. It operates over the World Wide Web using client-side applets, and can track user movement recovery over time and report it to a remote server computer. The system can also be customized to provide a personalized program of therapeutic exercise to a multiplicity of individuals. A mass manufactured computer input device senses the user's movement and provides a means to track sensory motor performance.

Abstract: An apparatus and method for utilizing any arbitrary mixture ratio of multiple fuel gases having differing combustion characteristics, such as natural gas and hydrogen gas, within an internal combustion engine. The gaseous fuel composition ratio is first sensed, such as by thermal conductivity, infrared signature, sound propagation speed, or equivalent mixture differentiation mechanisms and combinations thereof which are utilized as input(s) to a “multiple map” engine control module which modulates selected operating parameters of the engine, such as fuel injection and ignition timing, in response to the proportions of fuel gases available so that the engine operates correctly and at high efficiency irrespective of the gas mixture ratio being utilized. As a result, an engine configured according to the teachings of the present invention may be fueled from at least two different fuel sources without admixing constraints.

Abstract: A mathematical contouring algorithm that automatically determines the planning volume of a sarcoma prior to designing a brachytherapy treatment plan. The algorithm, utilizing computational geometry, numerical interpolation and artificial intelligence (AI) techniques, returns the planning volume in digitized and graphical forms in a matter of minutes. Such an automatic procedure reduces labor time and provides a consistent and objective method for determining planning volumes. In addition, a definitive representation of the planning volume allows for sophisticated brachytherapy treatment planning approaches to be applied when designing treatment plans, so as to maximize local tumor control and minimize normal tissue complications.

Abstract: Primitive hematopoietic stem cells are closely associated with discrete in vivo microenvironments. These “niches” are thought to provide the molecular signals that mediate stem cell differentiation and self renewal. We have dissected the fetal liver microenvironment into distinct cellular components by establishing an extensive panel of stromal cell lines. One particular cell line maintains repopulating stem cells for prolonged in vitro culture periods. A subtraction cloning strategy has yielded a cDNA which encodes a cell surface glycoprotein with a restricted pattern of expression among stromal cell lines. This molecule, previously identified as dlk/Pref-1, contains EGF-like repeats which are related to those in the Notch/Delta/Serrate family of proteins. We have investigated the potential role of this molecule in hematopoietic stem/progenitor cell regulation.

Abstract: The present invention provides for a method for treating a subject suffering from a condition associated with an extracellular zinc sphingomyelinase activity which comprises administering to the subject an amount of a zinc sphingomyelinase inhibitor effective to decrease extracellular zinc sphingomyelinase activity in the subject and thereby treat the subject.

Abstract: A resonant microcavity display (20) having microcavity with a substrate (25), a phosphor active region (50) and front and rear reflectors (30 and 60). The front and rear reflectors may be spaced to create either a standing or treaveling eledtromagnetic wave to enhance the efificenty of the light transmission.

Abstract: A method of improving the spraying of liquid droplets from a spray device onto a surface which method comprises imparting a unipolar charge to the said liquid droplets by double layer charging during the spraying of the liquid droplets from the spray device, the unipolar charge being at a level such that the said droplets have a charge to mass ratio of at least ±1×10−4 C/kg, whereby the charged droplets of the liquid are mutually repelled thereby increasing the spread of the droplets from a central spray line extending from the head of the spray device and avoiding coalescence of the droplets, thus providing a more even coverage of the surface which is to be sprayed. In particular, the method enables liquid droplets to be sprayed onto a surface which is obscured by all object located between the surface and the spray device.

Abstract: A method for monitoring and treating a patient with one or more diagnosed conditions includes a few steps. A current assessment of each of the diagnosed conditions is determined in a treatment processing system. The current assessment is based on objective data and subjective data about each of the diagnosed conditions from the patient who is at a remote location and on one or more assessment guidelines for each of the diagnosed conditions. Next, an existing treatment plan for each of the diagnosed conditions is updated using the treatment processing system. The updated treatment plan is based on the existing treatment plan, the current assessment and on one or more treatment guidelines for each of the diagnosed conditions. The updated treatment plan for each of the diagnosed conditions is then transmitted to the patient for application by the patient at the remote location.

Abstract: The present invention relates generally to Activity Dependent Neurotrophic Factor III (ADNF III), also known as Activity Dependent Neuroprotective Protein (ADNP). More particularly, the present invention relates to nucleic acid sequences encoding ADNF III polypeptides; ADNF III polypeptides encoded by such nucleic acid sequences; antibodies to ADNF III polypeptides; and methods of using such ADNF III polypeptides for the treatment of neurological deficiencies and for the prevention of cell death associated with (1) gp120, the envelope protein from HIV; (2) N-methyl-D-aspartic acid (excito-toxicity); (3) tetrodotoxin (blockage of electrical activity); and (4) &bgr;-amyloid peptide, a substance related to neuronal degeneration in Alzheimer's disease.

Abstract: A method for reproducing an integral, panoramogramic or full spatial image for viewing using a decoding screen as a 3-D picture comprising representing the image as an array of image points with a density corresponding to high resolution ink printing.

Abstract: The present invention provides a device for testing the abrasiveness of paper and other sheet materials. The device comprises a punching module, a feed module for moving the material, a holding module that holds the material while the punching module penetrated the material, and an instrument that records and analyzes the amount of force needed to punch through the material and then calculates the abrasive properties of the material. The punching module comprises a punching instrument for punching the material, a driving mechanism for operating the punching instrument, and a measurement system that measures the amount of the force required to punch the material. A method for using the device is also provided.

Abstract: Forming an interconnect of a semiconductor device includes defining a recessed structure proximate to an outer surface of a substrate of a semiconductor device. A metal layer is deposited within the recessed structure. A region of the metal layer is exposed to a plasma operable to react with the region of the metal layer. A metal compound layer is formed from the region of the metal layer by reacting the region of the metal layer with the plasma. The metal compound layer is removed from the semiconductor structure to yield a remaining metal layer. An interconnect of the semiconductor device is formed from the remaining metal layer.

Abstract: Determinations are made related to the presence of a predetermined material in concrete under test using previously obtained model information. In one embodiment, the predetermined material includes a chloride material and the model information is obtained using a number of cured cement specimens. The model information is stored in memory, such as in the form of a look-up table. When the concrete is being inspected, one or more magnitudes of reflections coefficients are measured and such is utilized with the model information to make determinations related to the presence of the predetermined material. In developing the model information, each of the plurality of cured cement specimens is located in a bath containing the predetermined material. The bath may be pressurized. The cured cement specimens are maintained in the bath for different, known time intervals. After the known time interval for a particular specimen, it is dried and one or more magnitudes of reflection coefficients are measured.

Abstract: The present invention is directed to an asexually reproduced variety of perennial Zoysia matrella. The inventive variety demonstrates a unique combination of characteristics including white stigmas, purple-brown anthers, good shade tolerance and turf quality, and resistance to hunting billbug, fall armyworm, yellow patch disease, Rhizoctonia blight and zoysiagrass rust disease.