Patents Assigned to Universtiy of Delaware
  • METHOD OF GENERATING LINKAGE-SPECIFIC DI-AND POLYPROTEIN PROBES
    Publication number: 20160237471
    Abstract: Deubiquitinating enzyme (DUB) probes are provided that resemble native diubiquitin (diUB) with a similar linkage size and that may contain a Michael acceptor for trapping the DUB active-site cysteine. For example, both K63- and K48-linked diubiquitin probes are generated using a facile chemical ligation method, utilizing the linker compound 3-(2-(bromomethyl)-1,3-dioxolan-2-yl)prop-2-en-1-amine. The diUb probes are capable of labelling DUBs from different families and may be employed to reveal intrinsic linkage specificities of DUBs.
    Type: Application
    Filed: October 15, 2014
    Publication date: August 18, 2016
    Applicant: Universtiy of Delaware
    Inventors: Zhihao ZHUANG, Guorui LI