Patents Assigned to Upside Foods, Inc.
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Patent number: 11976302Abstract: Provided herein are methods to increase the culture density and/or thickness of a cellular biomass in a cultivation infrastructure, to improve the culture of cells in the absence of serum in a cultivation infrastructure, and to promote anchorage-independent growth of a cellular biomass in a cultivation infrastructure. The methods comprise inhibiting the HIPPO signaling pathway, for example, by activating YAP1, activating TAZ, and/or inhibiting MOB1, LATS1 kinase, LATS2 kinase, WW45, MST1 kinase, and/or MST2 kinase in the cellular biomass. In some embodiments, the cellular biomass is harvested from the cultivation infrastructure for the formulation of cell-based food products or ingredients, such as animal meat manufactured from cells in an ex vivo process or for therapeutic applications such as organ or tissue transplantation or grafting.Type: GrantFiled: May 5, 2018Date of Patent: May 7, 2024Assignee: Upside Foods, Inc.Inventors: Nicholas J. Genovese, Meri Teresa Firpo, Daphné Dambournet
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Patent number: 11959054Abstract: The present disclosure relates to a substrate apparatus (and methods of manufacturing the same) with one or more substrates having a substrate spacing for growing a cell mass. In particular embodiments, the disclosed substrate apparatus includes a substrate wound into a coiled configuration with an intra-coil spacing between coil layers. To provide the intra-coil spacing, certain implementations use a separator. For example, a separator is applied to a substrate surface, and the separator-substrate combination is wound together (e.g., around a spool). In turn, a locking element is attached to the substrate to maintain the coiled configuration. The separator is then removed via heat treatment, chemical treatment, or physical displacement—thereby leaving the intra-coil spacing between the coil layers. Alternatively, no separator is used to provide a substrate spacing. For example, in lieu of a separator, the locking element is actively applied to the substrate during the winding process.Type: GrantFiled: November 4, 2021Date of Patent: April 16, 2024Assignee: Upside Foods, Inc.Inventors: Ryan Jiajong Liu, Jaewon Samuel Kang
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Patent number: 11912967Abstract: Described herein are novel systems and methods for biomanufacturing, such as tissue cultivation. In some variations the systems and methods may comprise at least one porous substrate.Type: GrantFiled: December 22, 2020Date of Patent: February 27, 2024Assignee: Upside Foods, Inc.Inventors: Napat Tandikul, Nicholas J. Genovese
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Patent number: 11898127Abstract: This disclosure describes methods for growing cells in a controlled environment in a growing room, transporting the cells to a harvest room, and removing the cells from the controlled environment in the harvest room. The disclosed method can limit exposure of cells to contaminants by maintaining a controlled environment through cell growth and additional processing. For instance, the cells may be washed and cooled within the controlled environment. The cells are transferred to a harvest room where they are harvested and removed from the controlled environment.Type: GrantFiled: September 26, 2022Date of Patent: February 13, 2024Assignee: Upside Foods, Inc.Inventors: Matthew Leung, Konrad Müller-Auffermann
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Patent number: 11891596Abstract: Provided herein are filter cake-based systems and methods for cultivating cells and cell biomass therefrom. Provided herein is a system for cultivating cells and cell biomass comprising a filter chamber comprising at least one inlet and at least one outlet, at least one filter support located within the filter chamber, and a filter cake located on the filter support, wherein the filter cake comprises at least one filter aid and a plurality of cells. Provided herein is a method for optimizing the cultivation of cells and cell biomass, comprising providing a filter support, adding at least one filter aid to the filter support, adding a plurality of cells to the filter aid, wherein the cells and the filter aid together comprise a filter cake, growing the cells into a cell biomass in the filter cake, wherein the filter cake is at least partially compressible.Type: GrantFiled: March 29, 2022Date of Patent: February 6, 2024Assignee: Upside Foods, Inc.Inventor: Konrad Mueller-Auffermann
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Publication number: 20230397633Abstract: Provided herein are cell-based meat compositions suitable for consumption comprising cells harvested from suspension cell culture and comprising an emulsion mixture. Also provide here are methods of making a cell-based meat composition suitable for consumption comprising: culturing a population of cells in suspension; harvesting the population of cells to produce a cell mass; formulating an emulsion mixture by: (i) emulsifying an oil with a hydrocolloid to produce an oil:hydrocolloid mixture; and (ii) adding the cell mass to the oil:hydrocolloid mixture to form an emulsion mixture; and (d) forming the cell-based meat composition into a patty.Type: ApplicationFiled: June 13, 2023Publication date: December 14, 2023Applicant: Upside Foods, Inc.Inventor: Daniel DAVILA
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Publication number: 20230399620Abstract: Provided herein are methods relating to making non-natively occurring myogenic cells. For example, provided herein are methods for transdifferentiating a liver-derived cell to a non-natively occurring myogenic cell. In another example, the method relates to using dedifferentiation to make non-natively occurring myogenic cells.Type: ApplicationFiled: June 13, 2023Publication date: December 14, 2023Applicant: Upside Foods, Inc.Inventors: Sukhdeep Singh DHADWAR, Neha ARORA, Rachel Michele SCHUMAKER
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Publication number: 20230392120Abstract: Provided herein are methods of engineering a cell line for reduced dependence on exogenous growth factor(s). In some embodiments, the method includes introducing into a cell one or more of: a polynucleotide comprising a coding sequence of a growth factor ligand; a polynucleotide comprising a coding sequence of a growth factor receptor; or a polynucleotide comprising a coding sequence of an activated downstream growth factor target, and culturing the cells in a cultivation infrastructure.Type: ApplicationFiled: June 7, 2023Publication date: December 7, 2023Applicant: Upside Foods, Inc.Inventors: Sukhdeep Singh DHADWAR, Rachel Michele SCHUMAKER
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Patent number: 11758931Abstract: The present disclosure provides methods and compositions useful for the production of slaughter-free meat products, and the characterizations of the same. The slaughter-free meat products contain several points of distinction when compared to conventional meat procured by harvesting the tissue of a dead animal. Such points of distinction include, but are not limited to, significantly reduced or substantially no: steroid hormones, antibiotics, or microbial contamination; lower fat content; no vasculature; and extended shelf life both at room temperature and when refrigerated.Type: GrantFiled: September 22, 2021Date of Patent: September 19, 2023Assignee: UPSIDE FOODS, INC.Inventors: Kevin J. Kayser, Morgan Laurence Rease, Mark E. Juhas, Jessica M. Joslin, Uma S. Valeti, Eric N. Schulze
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Patent number: 11760964Abstract: A plant fat-based scaffold for growing cell-based meat products for consumption. The scaffold comprises primarily plant fats or waxes in addition to cell binding proteins and optional additional components that assist in the growth of cultivated animal cells. The scaffold can exist in both a liquified state during sterilization and a solid state during the formation of the scaffold, the seeding of the cultivated cells, and the cellular growth phase. The scaffold is capable of remaining in the final product for consumption or is partially or completely melted out of the final product and recycled into raw material for forming new scaffolds.Type: GrantFiled: January 10, 2022Date of Patent: September 19, 2023Assignee: Upside Foods, Inc.Inventors: Konrad Muller-Aufferman, Michaela Walker
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Patent number: 11752509Abstract: An assembly for delivering a fluid includes a fluid dispenser connected to a fluid supply conduit. The fluid dispenser includes a fluid outlet positioned along a length of the fluid dispenser. The fluid outlet is configured to deliver the fluid from the fluid supply conduit at a flow rate that varies along the length of the fluid dispenser.Type: GrantFiled: June 17, 2021Date of Patent: September 12, 2023Assignee: Upside Foods, Inc.Inventors: Ryan Liu, Michelle Warner, Jaewon Samuel Kang, Matthew Leung, Eric Ordonez, Roshan Patel, Konrad Muller-Auffermann
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Patent number: 11752510Abstract: An assembly for delivering a fluid includes a fluid dispenser connected to a fluid supply conduit. The fluid dispenser includes a fluid outlet positioned along a length of the fluid dispenser. The fluid outlet is configured to deliver the fluid from the fluid supply conduit at a flow rate that varies along the length of the fluid dispenser.Type: GrantFiled: January 10, 2022Date of Patent: September 12, 2023Assignee: Upside Foods, Inc.Inventors: Ryan Liu, Michelle Warner, Jaewon Samuel Kang, Matthew Leung, Eric Ordonez, Roshan Patel, Konrad Muller-Auffermann
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Patent number: 11708587Abstract: Provided herein are compositions and methods to make and use engineered cells, for the purpose of increasing the cell density of a culture comprising metazoan cells and for the production of a cultured edible product.Type: GrantFiled: September 2, 2022Date of Patent: July 25, 2023Assignee: UPSIDE FOODS, INC.Inventors: Nicholas J. Genovese, Eric N. Schulze, Danielle Nicole Desmet
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Patent number: 11627751Abstract: The apparatuses described herein relate to preparation of a meat product. Apparatuses, systems comprising the apparatuses, and methods of making and use the systems and apparatuses are described herein. These are useful for controlling one or more of growth on and separation of a meat product from an enclosed substrate. The apparatuses and systems are configured to receive fluid and grow the meat product and/or separate the meat product from the substrate in a scalable manner.Type: GrantFiled: July 13, 2022Date of Patent: April 18, 2023Assignee: Upside Foods, Inc.Inventors: Matthew Leung, Michelle Warner, Ryan Edward Vanderpol, Thomas Pei-Ja Hsiu, Kathleen Carswell
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Patent number: 11576411Abstract: The apparatuses described herein relate to preparation of a meat product. Apparatuses, systems comprising the apparatuses, and methods of making and use the systems and apparatuses are described herein. These are useful for controlling one or more of growth on and separation of a meat product from an enclosed substrate. The apparatuses and systems are configured to receive fluid and grow the meat product and/or separate the meat product from the substrate in a scalable manner.Type: GrantFiled: September 8, 2021Date of Patent: February 14, 2023Assignee: Upside Foods, Inc.Inventors: Matthew Leung, Michelle Warner, Ryan Edward Vanderpol, Thomas Pei-Ja Hsiu, Kathleen Carswell
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Patent number: 11559072Abstract: The substrates, systems, and methods described herein relate to textured substrates for preparing a comestible meat product. Substrates and methods are described herein for controlling one or more of growth, adhesion, retention, and/or release of cells (e.g., of a cell sheet) on or from the surface of the substrate. A method of preparing a comestible meat product may include applying a plurality of non-human cells to at least one patterned texture substrate, growing the cells on the patterned texture substrate to form the comestible meat product, and separating the comestible meat product from the patterned texture substrate. The patterned texture allows for improved growth, adhesion, retention, and/or release of cells as compared to another surface not comprising the patterned texture. In some embodiments, the cell culture substrate surfaces include a plurality of regions corresponding to a plurality of patterned textures.Type: GrantFiled: April 21, 2022Date of Patent: January 24, 2023Assignee: Upside Foods, Inc.Inventors: Matthew Leung, Asha Godbole, George C Engelmayr, Jr., Nicholas J Genovese, Uma S Valeti, Kathleen Carswell
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Patent number: 11559073Abstract: The substrates, systems, and methods described herein relate to textured substrates for preparing a comestible meat product. Substrates and methods are described herein for controlling one or more of growth, adhesion, retention, and/or release of cells (e.g., of a cell sheet) on or from the surface of the substrate. A method of preparing a comestible meat product may include applying a plurality of non-human cells to at least one patterned texture substrate, growing the cells on the patterned texture substrate to form the comestible meat product, and separating the comestible meat product from the patterned texture substrate. The patterned texture allows for improved growth, adhesion, retention, and/or release of cells as compared to another surface not comprising the patterned texture. In some embodiments, the cell culture substrate surfaces include a plurality of regions corresponding to a plurality of patterned textures.Type: GrantFiled: April 21, 2022Date of Patent: January 24, 2023Assignee: Upside Foods, Inc.Inventors: Matthew Leung, Asha Godbole, George C Engelmayr, Jr., Nicholas J Genovese, Uma S Valeti, Kathleen Carswell
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Publication number: 20220401974Abstract: An assembly for delivering a fluid includes a fluid dispenser connected to a fluid supply conduit. The fluid dispenser includes a fluid outlet positioned along a length of the fluid dispenser. The fluid outlet is configured to deliver the fluid from the fluid supply conduit at a flow rate that varies along the length of the fluid dispenser.Type: ApplicationFiled: January 10, 2022Publication date: December 22, 2022Applicant: Upside Foods, Inc.Inventors: Ryan LIU, Michelle WARNER, Jaewon Samuel KANG, Matthew LEUNG, Eric ORDONEZ, Roshan PATEL, Konrad MULLER-AUFFERMANN
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Publication number: 20220403310Abstract: A plant fat-based scaffold for growing cell-based meat products for consumption. The scaffold comprises primarily plant fats or waxes in addition to cell binding proteins and optional additional components that assist in the growth of cultivated animal cells. The scaffold can exist in both a liquified state during sterilization and a solid state during the formation of the scaffold, the seeding of the cultivated cells, and the cellular growth phase. The scaffold is capable of remaining in the final product for consumption or is partially or completely melted out of the final product and recycled into raw material for forming new scaffolds.Type: ApplicationFiled: January 10, 2022Publication date: December 22, 2022Applicant: Upside Foods, Inc.Inventors: Konrad MULLER-AUFFERMAN, Michaela WALKER
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Publication number: 20220401973Abstract: An assembly for delivering a fluid includes a fluid dispenser connected to a fluid supply conduit. The fluid dispenser includes a fluid outlet positioned along a length of the fluid dispenser. The fluid outlet is configured to deliver the fluid from the fluid supply conduit at a flow rate that varies along the length of the fluid dispenser.Type: ApplicationFiled: June 17, 2021Publication date: December 22, 2022Applicant: Upside Foods, Inc.Inventors: Ryan LIU, Michelle WARNER, Jaewon Samuel KANG, Matthew LEUNG, Eric ORDONEZ, Roshan PATEL, Konrad MULLER-AUFFERMANN