Abstract: A method of manufacturing a frozen or partially-frozen product which method includes: a) providing a liquid material in a substantially sealed vessel; b) subjecting liquid material to a negative pressure; and c) subjecting the liquid material, whilst under the negative pressure, to a temperature substantially less than 0° C.
Abstract: The present invention relates to a method for purifying a recombinant glucose binding protein, in particular the lectin Concanavalin A (Con A). The method specifically utilizes a buffer in which impurities, such as glycogen and other substances are soluble, but in which the protein remains insoluble. The use of such buffers, and the purified proteins are also described.
Abstract: Methods for obtaining recombinant glucose binding proteins are provided. In particular methods for obtaining Concanavalin A are described. The use of the proteins in systems, e.g. for glucose determini ation are also provided.
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