Abstract: The present invention provides an isolated nucleic acid comprising a first nucleotide sequence encoding an amino acid sequence comprising at least three positively charged amino acid residues, positioned upstream and in frame with a second nucleotide sequence encoding a protein. In addition, the present invention provides an isolated nucleic acid comprising a first nucleotide sequence encoding a DNA binding protein, positioned upstream and in frame with a second nucleotide sequence encoding a protein. An isolated nucleic acid is also provided, which comprises a first nucleotide sequence encoding a bacteriophage lambda repressor protein, positioned upstream and in frame with a second nucleotide sequence encoding a protein.
Type:
Grant
Filed:
April 16, 1999
Date of Patent:
May 7, 2002
Assignee:
Va{umlaut over (n)}derbilt University
Inventors:
Richard M. Breyer, Lijun Ma, Chris Kennedy