Patents Assigned to Valentis, Inc.
  • Patent number: 6271209
    Abstract: Methods of transfecting cells in vivo, including tumor cells in the peritoneal cavity are provided. Related lipid:nucleic acid formulations adapted to transfecting cells in the peritoneal cavity are provided. Assays, including high-throughput assays for screening lipid:nucleic acids are also provided.
    Type: Grant
    Filed: April 1, 1999
    Date of Patent: August 7, 2001
    Assignee: Valentis, Inc.
    Inventors: Janet G. Smith, Ralph W. Niven, Yilin Zhang
  • Patent number: 6235310
    Abstract: Methods and compositions are provided for the introduction of polyanionic molecules, in particular, nucleic acids, into mammalian cells using certain phosphatidyl ethanolamines as helper lipids in conjunction with various cationic lipids. In particular, cationic lipid-mediated transfection of mammalian cells is improved by the use of lipid carriers comprising DLPE or DiPPE and cationic lipids.
    Type: Grant
    Filed: April 3, 1998
    Date of Patent: May 22, 2001
    Assignee: Valentis, Inc.
    Inventors: Jinkang Wang, Yi-Lin Zhang
  • Patent number: 6114169
    Abstract: Methods are provided for the preparation of transfection complexes of polynucleotides and polycations, especially cationic lipids, suitable for delivering polynucleotides to cells. In particular, methods are provided for preparing transfection complexes using a reduced-volume, dual-feed process. Complexes are formed upon the collision of two feed stream, containing polynucleotides and polycation, respectively, under conditions resulting in turbulent mixing conditions in minimal volume, and removal of transfection complexes under laminar flow conditions. Alternatively, the components are mixed in a static mixer. The process is easily scaleable and highly reproducible.
    Type: Grant
    Filed: October 23, 1998
    Date of Patent: September 5, 2000
    Assignee: Valentis, Inc.
    Inventors: Robert Bridenbaugh, Warren Dang, Gary Koe