Abstract: A method of determining variation between a query cell culture media and a reference cell culture media is provided. The method comprises the steps of incubating a reference cell with an aliquot of the query culture media and at least three chemical cell stressors in wells of a microtitre plate and determining an environmental response of the reference cell in the presence of the query cell culture media and each of the plurality of chemical cell stressors. A query cell culture media specific environmental response fingerprint is generated comprising the plurality of chemical cell stressor specific environmental responses. The query cell culture media-specific environmental response fingerprint is compared with a reference media-specific environmental response fingerprints corresponding to a reference cell culture media and generated using the reference cell.

Abstract: A method for predicting the phenotypic stability of a cell line comprises the steps of culturing the cell line over an assay period, and generating an environmental response fingerprint for the cell line at a plurality of different time points over the assay period, wherein each environmental response fingerprint is generated by determining the environmental response of the cells in the presence of each of a plurality of chemical cell stressors. The plurality of environmental response fingerprints are compared to detect change in the environmental response fingerprint over the assay period. The level of change in the environmental response fingerprint over the assay period is indicative of the level of predicted phenotypic instability in the cell line. The environmental response may be growth response, productivity response, or another detectable environmental response.

Abstract: “A rapid, high throughput, method of predicting relative fed batch performance of a panel of clonal cells derived from a single host cell line”. A computer implemented method of predicting relative fed batch production titer of a panel of clonal producer cells derived from a single parental host cell population comprises the steps of assaying a level of growth of each clone in the presence and absence of at least three individual chemical cell stressors, comparing the level of growth of each clone in the presence and absence of the at least three chemical cell stressors to provide a normalised production titer response for each clone in each stressed microenvironment, and inputting a clone-specific production titer response profile comprising the normalised production titer response for each clone in each stressed microenvironments into a computational model.

Abstract: A method of determining or predicting a characteristic of a query cell, for example the identity of the query cell, is described. The method comprises the steps of incubating the query cell with a plurality of chemical cell stressor molecules, determining the growth response of the query cell in the presence of each of the at least three chemical cell stressor molecules to generate a query cell-specific growth response fingerprint, and comparing the query cell-specific growth response fingerprint with one or more reference cell-specific growth response fingerprints corresponding to one or more cells having known characteristics. The characteristic of the cell (for example the identity of the cell) may be determined based on the level of correlation between the query cell-specific growth response fingerprint and the one or more reference cell-specific growth response fingerprints.