Abstract: The present invention is related to a process of producing ethanol and gluten from cereals especially from wheat, which comprises the steps if:(a) crushing cereals and soaking the thus crushed cereals in water;(b) mechanically separating the mixture obtained in step (a) so as to obtain a sediment which contains a major part of the insoluble substances, and a suspension which contains at least 60% of the initially present nitrogen-containing substances;(c) subsequently separating the thus produced suspension of step (b), so as to isolate an insoluble fraction containing more than 50% of the initially present proteins and consisting of gluten of said cereals and of a supernatent fraction;(d) mixing the sediment obtained in step (b) with said supernatent fraction of step (c) so as to produce a final suspension containing at least 90% of the initial starch;(e) submitting the starch contained in said final suspension of step (d) to enzymatic hydrolysis;(f) fermenting the suspension as hydrolysed in step (e), while