Patents Assigned to Vanadis Diagnostics
-
Patent number: 11591639Abstract: This disclosure provides, inter alia, a probe system probe system for analyzing a nucleic acid sample. In some embodiments, the probe system may comprise: a set of identifier oligonucleotides of sequence B, a set of splint oligonucleotides of formula X?-A?-B?-Z?, wherein sequence A? is complementary to a genomic fragment and sequence B? is complementary to at least one member of the set of identifier oligonucleotides, and one or more probe sequences comprising X and Z. Each splint oligonucleotide is capable of hybridizing to the probe sequences, a member of the set of identifier oligonucleotides and a genomic fragment, thereby producing a ligatable complex of formula X-A-B-Z. The probe system can be used to identify a chromosome aneuploidy in cell free DNA, for example.Type: GrantFiled: September 30, 2020Date of Patent: February 28, 2023Assignee: VANADIS DIAGNOSTICSInventors: Carl Oscar Fredrik Dahl, Olof John Ericsson, Filip Karlsson, Fredrik Roos
-
Patent number: 11365440Abstract: This disclosure provides, among other things, a method for processing a membrane comprising rolling circle amplification (RCA) products. In some embodiments, this method may comprise: (a) obtaining a porous capillary membrane that comprises fluorescently labeled RCA products that are in or on the membrane; (b) depositing a curable polymer onto the membrane; and (c) curing the curable polymer to encapsulate the RCA products in a solid. In some embodiments, the curable polymer may be a silicone and may be transparent in its solid form. A kit for performing the method and a composition made by the method are also provided.Type: GrantFiled: August 6, 2020Date of Patent: June 21, 2022Assignee: VANADIS DIAGNOSTICSInventors: Mathias Howell, Ove Öhman, Fredrik Persson, Linus Olausson
-
Patent number: 11117128Abstract: This disclosure provides, among other things, a filtration device comprising an open bottomed multi-well plate, a planar spacer that comprises apertures, and a porous capillary membrane. In the device, the planar spacer is sandwiched between the multi-well plate and the porous capillary membrane and the planar spacer is bonded to both the multi-well plate and the porous capillary membrane via an adhesive. Kits and methods of making the device are also provide.Type: GrantFiled: August 22, 2018Date of Patent: September 14, 2021Assignee: VANADIS DIAGNOSTICS ABInventor: Mathias Howell
-
Patent number: 10934579Abstract: A method of sample analysis is provided. In certain embodiments, the method may comprise: (a) filtering a liquid sample containing rolling circle amplification (RCA) products using a porous capillary membrane, thereby producing an array of the RCA products on the membrane; wherein the sample contains at least a first population of RCA products and a second population of RCA products, wherein the first and second populations of labeled RCA products are distinguishably labeled; and (b) determining the amount of the first labeled population of RCA products and the amount of the second labeled population of RCA products in an area of the membrane.Type: GrantFiled: January 11, 2019Date of Patent: March 2, 2021Assignee: VANADIS DIAGNOSTICSInventors: Ove Öhman, Fredrik Persson, Mathias Howell
-
Patent number: 10876169Abstract: A method of estimating the amount of a methylated locus is provided. In certain embodiments the method comprises: digesting a nucleic acid sample that contains both unmethylated and methylated copies of a genomic locus with an MspJI family member to produce a population of fragments that are in the range of 20-40 nucleotides in length, ligating adaptor sequence A and adaptor sequence B to the respective ends of a target fragment of sequence X, and quantifying the amount of ligation products of formula A-X-B. A kit for performing the method is also provided.Type: GrantFiled: December 4, 2018Date of Patent: December 29, 2020Assignee: VANADIS DIAGNOSTICSInventors: Carl Oscar Fredrik Dahl, Olof John Ericsson, Johan Banér
-
Patent number: 10822640Abstract: This disclosure provides, inter alia, a probe system probe system for analyzing a nucleic acid sample. In some embodiments, the probe system may comprise: a set of identifier oligonucleotides of sequence B, a set of splint oligonucleotides of formula X?-A?-B?-Z?, wherein sequence A? is complementary to a genomic fragment and sequence B? is complementary to at least one member of the set of identifier oligonucleotides, and one or more probe sequences comprising X and Z. Each splint oligonucleotide is capable of hybridizing to the probe sequences, a member of the set of identifier oligonucleotides and a genomic fragment, thereby producing a ligatable complex of formula X-A-B-Z. The probe system can be used to identify a chromosome aneuploidy in cell free DNA, for example.Type: GrantFiled: November 8, 2019Date of Patent: November 3, 2020Assignee: VANADIS DIAGNOSTICSInventors: Carl Oscar Fredrik Dahl, Olof John Ericsson, Filip Karlsson, Fredrik Roos
-
Patent number: 10781476Abstract: This disclosure provides, among other things, a method for processing a membrane comprising rolling circle amplification (RCA) products. In some embodiments, this method may comprise: (a) obtaining a porous capillary membrane that comprises fluorescently labeled RCA products that are in or on the membrane; (b) depositing a curable polymer onto the membrane; and (c) curing the curable polymer to encapsulate the RCA products in a solid. In some embodiments, the curable polymer may be a silicone and may be transparent in its solid form. A kit for performing the method and a composition made by the method are also provided.Type: GrantFiled: October 4, 2017Date of Patent: September 22, 2020Assignee: VANADIS DIAGNOSTICSInventors: Mathias Howell, Ove Öhman, Fredrik Persson, Linus Olausson
-
Patent number: 10731214Abstract: Provided herein, among other things, is a method of processing a nucleic acid sample. In some embodiments, the method comprises a) hybridizing a sample comprising a target fragment to a nucleic acid probe comprising: i. a head sequence and a tail sequence, wherein the head and tail sequences are at the ends of a first oligonucleotide molecule; and ii.Type: GrantFiled: January 17, 2019Date of Patent: August 4, 2020Assignee: VANADIS DIAGNOSTICSInventors: Carl Oscar Fredrik Dahl, Olof John Ericsson
-
Patent number: 10526643Abstract: Described herein is a new approach in which a nucleic acid species of interest (e.g. a chromosome) containing multiple unique target sequences is detected using multiple specific probes that are amplified by rolling circle amplification and detected. Multiple probes are used to provide a detectable signal, where the magnitude of the signal is proportional to the number of probes recognising their target sequences. Individual signals from the plurality of probes are converted into a single cumulative detectable signal, amplifying the individual signals through the multiplex probing. Ten or more probes produce a signal amplification of ten-fold or more. The generated signals depend on correctly reacted probes upon target recognition, using sequence specific hybridisation and enzymatic catalysis to generate specific products from which the signal is obtained.Type: GrantFiled: November 26, 2014Date of Patent: January 7, 2020Assignee: Vanadis DiagnosticsInventors: Carl Oscar Fredrik Dahl, Olof John Ericsson
-
Patent number: 10508300Abstract: This disclosure provides, inter alia, a probe system probe system for analyzing a nucleic acid sample. In some embodiments, the probe system may comprise: a set of identifier oligonucleotides of sequence B, a set of splint oligonucleotides of formula X?-A?-B?-Z?, wherein sequence A? is complementary to a genomic fragment and sequence B? is complementary to at least one member of the set of identifier oligonucleotides, and one or more probe sequences comprising X and Z. Each splint oligonucleotide is capable of hybridizing to the probe sequences, a member of the set of identifier oligonucleotides and a genomic fragment, thereby producing a ligatable complex of formula X-A-B-Z. The probe system can be used to identify a chromosome aneuploidy in cell free DNA, for example.Type: GrantFiled: September 16, 2016Date of Patent: December 17, 2019Assignee: Vanadis DiagnosticsInventors: Carl Oscar Fredrik Dahl, Olof John Ericsson, Filip Karlsson, Fredrik Roos
-
Patent number: 10240198Abstract: Provided herein, among other things, is a method of processing a nucleic acid sample. In some embodiments, the method comprises a) hybridizing a sample comprising a target fragment to a nucleic acid probe comprising: i. a head sequence and a tail sequence, wherein the head and tail sequences are at the ends of a first oligonucleotide molecule; and ii.Type: GrantFiled: November 26, 2014Date of Patent: March 26, 2019Assignee: Vanadis DiagnosticsInventors: Carl Oscar Fredrik Dahl, Olof John Ericsson
-
Patent number: 10208336Abstract: A method of sample analysis is provided. In certain embodiments, the method may comprise: (a) filtering a liquid sample containing rolling circle amplification (RCA) products using a porous capillary membrane, thereby producing an array of the RCA products on the membrane; wherein the sample contains at least a first population of RCA products and a second population of RCA products, wherein the first and second populations of labeled RCA products are distinguishably labeled; and (b) determining the amount of the first labeled population of RCA products and the amount of the second labeled population of RCA products in an area of the membrane.Type: GrantFiled: May 2, 2016Date of Patent: February 19, 2019Assignee: Vanadis DiagnosticsInventors: Ove Öhman, Fredrik Persson, Mathias Howell
-
Patent number: 10174383Abstract: A method of estimating the amount of a methylated locus is provided. In certain embodiments the method comprises: digesting a nucleic acid sample that contains both unmethylated and methylated copies of a genomic locus with an MspJI family member to produce a population of fragments that are in the range of 20-40 nucleotides in length, ligating adaptor sequence A and adaptor sequence B to the respective ends of a target fragment of sequence X, and quantifying the amount of ligation products of formula A-X-B. A kit for performing the method is also provided.Type: GrantFiled: July 30, 2015Date of Patent: January 8, 2019Assignee: Vanadis DiagnosticsInventors: Carl Oscar Fredrik Dahl, Olof John Ericsson, Johan Banér