Patents Assigned to Vanadis Diagnostics
  • Patent number: 10526643
    Abstract: Described herein is a new approach in which a nucleic acid species of interest (e.g. a chromosome) containing multiple unique target sequences is detected using multiple specific probes that are amplified by rolling circle amplification and detected. Multiple probes are used to provide a detectable signal, where the magnitude of the signal is proportional to the number of probes recognising their target sequences. Individual signals from the plurality of probes are converted into a single cumulative detectable signal, amplifying the individual signals through the multiplex probing. Ten or more probes produce a signal amplification of ten-fold or more. The generated signals depend on correctly reacted probes upon target recognition, using sequence specific hybridisation and enzymatic catalysis to generate specific products from which the signal is obtained.
    Type: Grant
    Filed: November 26, 2014
    Date of Patent: January 7, 2020
    Assignee: Vanadis Diagnostics
    Inventors: Carl Oscar Fredrik Dahl, Olof John Ericsson
  • Patent number: 10508300
    Abstract: This disclosure provides, inter alia, a probe system probe system for analyzing a nucleic acid sample. In some embodiments, the probe system may comprise: a set of identifier oligonucleotides of sequence B, a set of splint oligonucleotides of formula X?-A?-B?-Z?, wherein sequence A? is complementary to a genomic fragment and sequence B? is complementary to at least one member of the set of identifier oligonucleotides, and one or more probe sequences comprising X and Z. Each splint oligonucleotide is capable of hybridizing to the probe sequences, a member of the set of identifier oligonucleotides and a genomic fragment, thereby producing a ligatable complex of formula X-A-B-Z. The probe system can be used to identify a chromosome aneuploidy in cell free DNA, for example.
    Type: Grant
    Filed: September 16, 2016
    Date of Patent: December 17, 2019
    Assignee: Vanadis Diagnostics
    Inventors: Carl Oscar Fredrik Dahl, Olof John Ericsson, Filip Karlsson, Fredrik Roos
  • Patent number: 10240198
    Abstract: Provided herein, among other things, is a method of processing a nucleic acid sample. In some embodiments, the method comprises a) hybridizing a sample comprising a target fragment to a nucleic acid probe comprising: i. a head sequence and a tail sequence, wherein the head and tail sequences are at the ends of a first oligonucleotide molecule; and ii.
    Type: Grant
    Filed: November 26, 2014
    Date of Patent: March 26, 2019
    Assignee: Vanadis Diagnostics
    Inventors: Carl Oscar Fredrik Dahl, Olof John Ericsson
  • Patent number: 10208336
    Abstract: A method of sample analysis is provided. In certain embodiments, the method may comprise: (a) filtering a liquid sample containing rolling circle amplification (RCA) products using a porous capillary membrane, thereby producing an array of the RCA products on the membrane; wherein the sample contains at least a first population of RCA products and a second population of RCA products, wherein the first and second populations of labeled RCA products are distinguishably labeled; and (b) determining the amount of the first labeled population of RCA products and the amount of the second labeled population of RCA products in an area of the membrane.
    Type: Grant
    Filed: May 2, 2016
    Date of Patent: February 19, 2019
    Assignee: Vanadis Diagnostics
    Inventors: Ove Öhman, Fredrik Persson, Mathias Howell
  • Patent number: 10174383
    Abstract: A method of estimating the amount of a methylated locus is provided. In certain embodiments the method comprises: digesting a nucleic acid sample that contains both unmethylated and methylated copies of a genomic locus with an MspJI family member to produce a population of fragments that are in the range of 20-40 nucleotides in length, ligating adaptor sequence A and adaptor sequence B to the respective ends of a target fragment of sequence X, and quantifying the amount of ligation products of formula A-X-B. A kit for performing the method is also provided.
    Type: Grant
    Filed: July 30, 2015
    Date of Patent: January 8, 2019
    Assignee: Vanadis Diagnostics
    Inventors: Carl Oscar Fredrik Dahl, Olof John Ericsson, Johan Banér