Abstract: Surgical assemblies and related methods are provided for using an actuator to deploy an coupler configured to close a tissue puncture or natural opening in the body, or to create an anastomosis. The surgical method includes deploying first and second anastomotic couplers within a patient to create anastomoses in the body to aid in the treatment of various ailments. The first coupler is deployed to fluidly join a gall bladder and an ileum, and the second coupler is deployed to fluidly join a proximal ileal loop and a distal ileal loop. Fluid can flow between central lumens of the first and second couplers.