Abstract: Botanical extracts are provided, which comprise ethanol extracts of Nannochloropsis algae having between 10 wt % and 50 wt % polar lipids and between 45 wt % and 60 wt % fatty acids, wherein the fatty acids comprise more than 10 wt % of eicosapentaenoic acid (EPA) as triglyceride conjugates and/or diglyceride conjugates, and less than 15 wt % EPA as free fatty acids. EPA level may reach up to 90 wt %, and the botanical extract may be fluid at room temperature. Growth conditions are configured, adjusted and monitored to promote EPA formation in the cell vacuoles, resulting in a high proportion of EPA as tri/di-glyceride conjugates, as well as in a high concentration of ?-3 fatty acids, which merely requires gentle extraction procedures to reach the final products, thereby maintaining the biochemical of the compounds without excessive modifications.

Abstract: Aspects of the invention are directed to a system and method of sparging an algae cultivation container. The method may include controlling at least one first sparger to distribute a first fluid into the container at a first operating flow rate; and controlling at least one second sparger to distribute a second fluid into the container at a second operating flow rate. The first operating flow rate may be adapted to allow mixing the algae in the cultivation container, and the second operating flow rate may be adapted to allow assimilation of materials in a liquid in the cultivation container.

Abstract: Botanical extracts are provided, which comprise ethanol extracts of Nannochloropsis algae having between 1 wt % and 50 wt % polar lipids and between 45 wt % and 60 wt % fatty acids, wherein the fatty acids comprise more than 10 wt % of eicosapentaenoic acid (EPA) as triglyceride conjugates and/or diglyceride conjugates, and less than 15 wt % EPA as free fatty acids. EPA level may reach up to 90 wt %, and the botanical extract may be fluid at room temperature. Growth conditions are configured, adjusted and monitored to promote EPA formation in the cell vacuoles, resulting in a high proportion of EPA as tri/di-glyceride conjugates, as well as in a high concentration of ?-3 fatty acids, which merely requires gentle extraction procedures to reach the final products, thereby maintaining the biochemical of the compounds without excessive modifications.

Abstract: Spirulina extracts and/or fractionated compounds thereof, sublingual spray formulation thereof, spraying devices for using the formulation, as well as methods of preparing and using the spirulina extracts to treat TNF-? related inflammation are provided. The spirulina extracts are prepared by water extraction of Arthrospira spp. biomass that is cultivated under controlled, ultra-high-density conditions with strong UV illumination and strong continuous mixing, and are characterized by high levels of c-phycocyanin, sorbitol and adenosine derivates—which were found to have a strong low-dose effect of reducing TNF-? secretion. The spirulina extracts may correspondingly be used to prevent or alleviate cytokine storm related to various infections or autoimmune diseases.

Abstract: An algae cultivation system may include: a plurality of panels within a cultivation container, positioned along a first axis perpendicular to the gravitational force, wherein a cultivation volume is created between each pair of panels, and wherein the cultivation volumes are fluidly coupled so as to allow horizontal flow therebetween along the first axis; at least one first sparger, to distribute a first fluid into the container at a first operating flow rate; at least one second sparger, to distribute a second fluid into the container at a second operating flow rate; and at least one controller, to control the first operating flow rate and the second operating flow rate. The first operating flow rate may be adapted to allow turbulent mixing the algae in the cultivation container, and the second operating flow rate may be adapted to allow assimilation of materials in a liquid in the cultivation container.

Abstract: A nutritional supplement is disclosed. The nutritional supplement may be included in a fish diet. The nutritional supplement may include a carrier oil; 22-30 wt. % Omega 3; and 8-20 wt. % Omega 7. In some embodiments, the carrier oil may be a vegetable oil. In some embodiments, the Omega 3 includes EPA and DHA fatty acids. In some embodiments, the Omega 3 includes is a mixture of EPA and DHA having ratios in the range of 1:10 to 10:1 wt. %.

Abstract: A composition for treating insomnia and related disorders is disclosed. The composition may include: (i) at least one polar lipid comprising at least one poly-unsaturated fatty acid (PUFA) moiety and (ii) Cannabidiol (CBD) or a derivative thereof.

Abstract: A method of growing algae in a cultivation container is disclosed. In some the method may include: circulating, via the cultivation container, in a closed loop, a first predetermined amount of gas mixture comprising a first type of gas and at least one second type of gas, the gas mixture may enter the container via one or more entrance spargers and exit via at least one exit pipe, the first type of gas may contain CO2 at a known first amount; receiving signal indicative of the amount of CO2 or H2S, in the gas mixture; when the signal indicates that the amount of CO2 drops below a first predetermined level or when the signal indicates that the amount of H2S rises above a first predetermined level, extracting a second predetermined amount of the gas mixture from the cultivation container: and adding an amount of the first type of gas to the gas mixture, equal to the second predetermined amount.

Abstract: Disclosed is a non-toxic omega-3 rich extract and non-toxic miscella and a method of producing same. The method may include obtaining an aqueous microalgae slurry comprising at least 65% water; mixing the aqueous microalgae slurry with ethanol for a predetermined duration; separating the aqueous microalgae slurry-ethanol mixture to liquids and miscella; and evaporating the water and the ethanol from the liquid to receive a liquid extract. In some embodiments, the miscella contains organic material and ash at an amount below 15 dry weight %.

Abstract: A device for storing live algae is disclosed. The device may include: a closed container, at least partially transparent to light, the container is configured to hold live algae aquaculture at a predetermined temperature; at least one light source for providing light to the closed container; a CO2 source for providing CO2 to the closed container; an air circulation system for circulating air inside the closed container; and a controller for controlling the at least one light source to illuminate an internal space of the closed container in an amount sufficient to keep the algae aquaculture alive but inhibits reproduction of the algae for at least 4 weeks.