Abstract: The present disclosure generally relates to methods for detecting one or more analytes in a sample using immunoassays. In some embodiments, the disclosure provides methods that include quenching the free secondary antibody with a fractionated serum composition. In some other aspects, the disclosure provides systems and kits suitable for carrying out the foregoing methods and any embodiments thereof.
Abstract: Reagents and methods for multi-step labeling of nucleic acids allow the addition of relatively insoluble or unstable labels to nucleic acid in the final step. Nucleic acids can be stored as a stable intermediate capable of reacting with a label conjugated to a thiol-reactive group.
Abstract: Reagents and methods for multi-step labeling of nucleic acids allow the addition of relatively insoluble or unstable labels to nucleic acid in the final step. Nucleic acids can be stored as a stable intermediate capable of reacting with a label conjugated to a thiol-reactive group.
Abstract: An enhanced labelling complex for localizing markers on a prepared tissue section is disclosed. The complex includes both avidin components and biotinylated macromolecular components at a ratio preselected to provide a complex which is sufficiently large to include a large number of labels, and sufficiently small to penetrate the tissue section. The markers are localized by first introducing a biotinylated link into the tissue section and thereafter exposing the section to the labelling complex.
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