Abstract: Provided is a cartridge for use in conducting diagnostic assays. The cartridge is configured to maintain sample fluids in a sealed manner and may be used to conduct one or more assays from a single patient sample within a single cartridge. Assays that may be conducted with the cartridge of the present invention include immunoassays and molecular assays. Methods of use for the cartridge and a system for using of the cartridge in combination with a module are also provided.