Abstract: The invention relates to a device for determining an analyte in a liquid sample. The inventive device consists of: a capillary action means, involving lateral migration, defining a reference capillary action direction and comprising a liquid sample deposit area and an analytedetection area which is disposed downstream of the deposit area; a first analytespecific binding reagent which is conjugated to a visible and/or measurable marker and which is free to migrate when wet by means of capillary action in the abovementioned capillary actions means along the reference direction; and a second analytespecific binding reagent which is immobilized in the detection area. The invention is characterized in that the detection area comprises the analyte or an analogue of the analyte, which is immobilized and disposed at a distance from the second specific binding reagent.

Abstract: The invention relates to a device for determining an analyte in a liquid sample. The inventive device consists of: a capillary action means, involving lateral migration, defining a reference capillary action direction and comprising a liquid sample deposit area and an analyte-detection area which is disposed downstream of the deposit area; a first analyte-specific binding reagent which is conjugated to a visible and/or measurable marker and which is free to migrate when wet by means of capillary action in the above-mentioned capillary actions means along the reference direction; and a second analyte-specific binding reagent which is immobilized in the detection area. The invention is characterized in that the detection area comprises the analyte or an analogue of the analyte, which is immobilized and disposed at a distance from the second specific binding reagent.

Abstract: The invention concerns a device for determining an analyte in a liquid sample, comprising: a) a gripping support (1); b) first capillary diffusing means (2) integral with the gripping support (1) comprising a downstream zone (2a) accessible to external observation; c) a set of predetermined reagents for detecting and/or quantifying the analyte; d) a member for collecting (4) the liquid sample mounted on the support (1). The invention is characterized in that the member for collecting (4) the liquid sample is mounted mobile or fixed on the support; second capillary diffusion means (41) extends from a zone collecting (41a) said sample, to a zone transferring (41b) the latter; and an upstream zone (2b) of said first capillary diffusion means (2) is arranged to be urged temporarily to constitute continuous capillary flow with the second diffusion means (41) transferring zone (41b), when the collecting member (4) is in the retracted position.

Abstract: The invention concerns solid-phase immunochromatographic methods for detecting an analyte in a liquid sample. In the inventive methods, a binding reagent conjugated with a particulate marker is extemporaneously added in liquid form.