Abstract: The invention discloses an indel molecular marker closely linked with pumpkin photoperiod insensitivity and application of the indel molecular marker, and belongs to the technical field of molecule detection. The indel molecular marker SEQ7593 is located on a tenth chromosome of a Cucurbita moschata, and is 280 bp in size. The nucleotide sequence of the indel molecular marker is shown as SEQ ID NO: 1. The indel molecular marker SEQ7593 can be directly used for creating an assistant breeding system of photoperiod insensitivity character molecular markers. Primer amplification designed according to the Indel molecular marker can be applied to assistant breeding of pumpkin breed improvement molecules in a simple, rapid and high-throughput manner, technical support is provided for pumpkin photoperiod insensitivity molecular breeding, and time for conventional gene positioning is shortened greatly.
Type:
Grant
Filed:
November 1, 2018
Date of Patent:
November 8, 2022
Assignee:
VEGETABLE RESEARCH INSTITUTE, GUANGDONG ACADEMY OF AGRICULTURAL SCIENCES
Abstract: The invention discloses an indel molecular marker closely linked with pumpkin photoperiod insensitivity and application of the indel molecular marker, and belongs to the technical field of molecule detection. The indel molecular marker SEQ7593 is located on a tenth chromosome of a Cucurbita moschata, and is 280 bp in size. The nucleotide sequence of the indel molecular marker is shown as SEQ ID NO.1. The indel molecular marker SEQ7593 can be directly used for creating an assistant breeding system of photoperiod insensitivity character molecular markers. Primer amplification designed according to the Indel molecular marker can be applied to assistant breeding of pumpkin breed improvement molecules in a simple, rapid and high-throughput manner, technical support is provided for pumpkin photoperiod insensitivity molecular breeding, and time for conventional gene positioning is shortened greatly.
Type:
Application
Filed:
November 1, 2018
Publication date:
June 25, 2020
Applicant:
VEGETABLE RESEARCH INSTITUTE, GUANGDONG ACADEMY OF AGRICULTURAL SCIENCES