Abstract: The present disclosure provides compositions and methods for repairing cartilage defects.

Abstract: The present disclosures describe devices and methods to repair cartilage defects using arthroscopic surgical methods. The disclosed devices include a cannula assembly including a cannula body, a dam seal sub-assembly disposed proximally from the cannula body, and an obturator inserted coaxially through both the cannula body and the dam seal sub-assembly; an articulated arthroscopic cutting tool; a ring curette; a square curette; a rake curette; a matrix shuttle delivery device for delivering a cell-seeded support matrix including chondrocytes; and an applicator tool.

Abstract: Described is a device comprising an anchor, a tray including a well adapted to receive the anchor, and a cover adapted to engage the tray and cover the well. The device may be used to form a membrane-cell matrix having a substantially uniform distribution of the cells on the membrane in at least two dimensions.

Abstract: The present invention provides a fluid exchange cell culture technique and tissue repair cells (TRCs) made by these methods, as well as methods using these cells. The method includes a new wash step which increases the tissue repair properties of the TRCs of the invention. This wash step allows for the production of TRC populations with greater tissue repair and anti-inflammatory capabilities. Embodiments of the present invention include a post-culture process for cultured cells that preferably includes the steps of: a wash process for removing unwanted residual culture components, a volume reduction process, and a harvesting process to remove cultured cells. Preferably, all these steps are performed within a aseptically closed cell culture chamber by implementing a separation method that minimizes mechanical disruption of the cells and is simple to automate. The harvested cells may then be concentrated to a final volume for the intended use.