Abstract: The invention relates to a method for the specific detection of a microorganism or a group of microorganisms via in situ hybridisation by means of flow cytometry.
Type:
Grant
Filed:
August 16, 2018
Date of Patent:
June 20, 2023
Assignee:
Vermicon AG
Inventors:
Jiri Snaidr, Claudia Beimfohr, Peter Muhlhahn
Abstract: The invention relates to a method for the specific detection of a microorganism or a group of microorganisms via in situ hybridisation by means of flow cytometry.
Type:
Application
Filed:
August 16, 2018
Publication date:
December 10, 2020
Applicant:
vermicon AG
Inventors:
Jiri SNAIDR, Claudia BEIMFOHR, Peter MÜHLHAHN
Abstract: The invention relates to a combined method for specifically identifying microorganisms by means of in situ hybridization and flow cytometry. The inventive method is particularly characterized by an improved specificity and a shorter duration of the process as opposed to methods known in prior art.
Type:
Application
Filed:
January 4, 2008
Publication date:
May 28, 2009
Applicant:
VERMICON AG
Inventors:
Jiri Snaidr, Claudia Beimfohr, Karin Thelen
Abstract: The invention relates to a method for the specific rapid detection of beverage-spoiling micro-organisms by means of in situ hybridisation. The invention also relates to specific oligonucleotide probes that are used in the detection method, and to kits containing said oligonucleotide probes.
Type:
Application
Filed:
September 23, 2004
Publication date:
January 31, 2008
Applicant:
VERMICON AG
Inventors:
Jiri Snaidr, Claudia Beimfohr, Karin Thelen, Angelika Lehner
Abstract: The invention relates to a method for detecting microorganisms in a sample by means of a nucleic acid probe. Conventional detection methods are, for example, the in-situ hybridization of microorganisms with fluorescence-labeled oligonucleotide probes (fluorescent in-situ hybridization). A disadvantage of said method is that an epifluorescence microscope is required for evaluating the results. According to the invention, the disadvantages of the in-situ hybridization method are overcome by hybridizing the microorganisms to be detected in a sample with a specific nucleic acid probe, removing non-hybridized nucleic acid probe molecules, separating and then detecting and optionally quantifying the hybridized nucleic acid probe molecules.