Abstract: In a method for determining trajectories of movable physical objects in a space on the basis of sensor data from a plurality of sensors, first data objects are generated based on the sensor data, wherein each first data object comprises at least one timestamp and one location specification of an object detected by at least one of the sensors, and a validation function. At least one model object, which represents a parameterization of a trajectory of one of the movable objects with at least one variable parameter, is provided. Then, a correspondence value is calculated for the first data objects, for an assignment of the respective data object to at least one of the model objects provided by way of evaluating the validation function of the data object for the model objects. An additional model object is provided and the data object is assigned to the additional model object if none of the correspondence values lie in a predetermined range.

Abstract: This invention relates to a method of determining presence, amount and/or activity of a clostridial neurotoxin in a sample, the method comprising or consisting of the following steps: (a) bringing said sample into contact with a liposome, said liposome comprising (aa) at least one receptor on its outer surface, said receptor being capable of binding said neurotoxin and comprising or consisting of (i) a glycolipid and (ii) a peptide or protein; and (ab) a substrate in its interior, said substrate (i) being cleavable by the peptidase comprised in said neurotoxin and (ii) generating a detectable signal upon cleavage, said detectable signal preferably being generated by (1) the donor of a FRET pair, said donor exhibiting increased fluorescence upon cleavage by said peptidase, (2) a luminescent compound formed upon said cleavage, or (3) an enzyme formed upon said cleavage; and (b) determining whether an increase in signal occurs as compared to the absence of said sample, wherein such increase is indicative of the