Abstract: A bioreactor including a housing, a first fluid dispenser and a second fluid dispenser. The bioreactor is configured to receive a scaffold mounted within the housing with the first and second fluid dispensers being positioned to apply respective first and second fluids to at least two different regions of a mounted scaffold.
Abstract: The invention provides a method for decellularising a tissue the method comprising the steps of: a) adding a tissue to be decellularised to a decellularisation vessel; b) adding a decellularisation medium comprising at least one detergent and/or at least one enzyme to the vessel; c) introducing a supercritical fluid to the vessel; and d) maintaining contact of the tissue with the supercritical fluid for a time sufficient to substantially decellularise the tissue.
Abstract: The invention provides a method for producing an implant from interstitial, connective or supporting tissue, the method comprising at least one step of perfusing the tissue with at least one decellularization medium under negative pressure applied for substantially the whole time period of the perfusion.
Type:
Grant
Filed:
June 16, 2014
Date of Patent:
April 24, 2018
Assignee:
Videregen Limited
Inventors:
Tahera Iqbal Ansari, Paul David Sibbons
Abstract: The invention provides a method for producing an implant from interstitial, connective or supporting tissue, the method comprising at least one step of perfusing the tissue with at least one decellularisation medium under negative pressure applied for substantially the whole time period of the perfusion.
Type:
Application
Filed:
June 16, 2014
Publication date:
April 7, 2016
Applicant:
VIDEREGEN LIMITED
Inventors:
Tahera Iqbal ANSARI, Paul David SIBBONS
Abstract: The invention relates to an implant derived from intestinal tissue and to an improved method for producing an implant from intestinal tissue. According to the invention, intestinal tissue comprising a tubular segment of intestine with at least part of its associated vasculature intact is processed by: perfusing the vasculature through a vessel thereof with at least one decellularising medium; and separately perfusing the tubular segment of intestine through its lumen with at least one decellularising medium. The new method greatly improves tissue processing as compared with the methods previously known in the art, in which decellularisation solutions were perfused into the tissue via the vasculature only, relying on diffusion to decellularise and purify the small bowel tissue.
Type:
Application
Filed:
July 16, 2013
Publication date:
July 9, 2015
Applicant:
Videregen Limited
Inventors:
Paul David Sibbons, Tahera Iqbal Ansari