Abstract: A recombinant Pichia pastoris strain which is genetically modified for highly expressing pediocin PA-1 and secreted into a medium so that the pediocin PA-1 is easily collected and purified from the culture medium. The obtained pediocin PA-1 has specific antimicrobial activities against Listeria monocytogenes ATCC 13932 and several other bacteria strains.

Abstract: The present invention provides novel reagents and a cloning procedure based on homologous recombination for the site-directed cloning of a DNA fragment to a vector at designed site(s). The cloning reagents are made of mixture of extracts from at least two different cell types, preferably a mixture made of extracts from wild-type E. coli and S. cerevisiae. Due to the activity of the mixture of cell extracts, recombination occurs between the 3? and 5?-ends of the target DNA and at the ends of linearized vector, which facilitates in-frame construction of expression vectors.

Abstract: A method and a computer software for managing a database of a computer network are disclosed which comprises: selecting a data sample, dividing the data sample into an N×M matrix having equal cells, grouping adjacent cells into a plurality of memory blocs by a least differential sum method, constructing a decision tree based on the partitions of the memory blocs, labeling each of the memory blocs based on the decision tree; storing and retrieving data points into the memory blocs; and linking the memory blocs together by a set of resolution nets.

Abstract: This invention provides a recombinant Pichia pastoris strain which has been genetically modified for highly expressing pediocin PA-1 and secreting it into the medium so that the pediocin PA-1 can be easily collected and purified from the culture medium. In this invention, we also demonstrated that the obtained pediocin PA-1 had specific antimicrobial activities against Listeria monocytogenes ATCC 13932 and several other bacteria strains. Therefore, pediocin PA-1 produced from this recombinant Pichia pastoris strain has a great potential for the application in food preservation and pharmaceutical industry.

Abstract: A method and a computer software for managing a database of a computer network are disclosed which comprises: selecting a data sample, dividing the data sample into an N×M matrix having equal cells, grouping adjacent cells into a plurality of memory blocs by a least differential sum method, constructing a decision tree based on the partitions of the memory blocs, labeling each of the memory blocs based on the decision tree; storing and retrieving data points into the memory blocs; and linking the memory blocs together by a set of resolution nets.

Abstract: The present invention provides novel reagents and a cloning procedure based on homologous recombination for the site-directed cloning of a DNA fragment to a vector at designed site(s). The cloning reagents are made of mixture of extracts from at least two different cell types, preferably a mixture made of extracts from wild-type E. coli and S. Cerevisiae. Due to the activity of the mixture of cell extracts, recombination occurs between the 3? and 5?-ends of the target DNA and at the ends of linearized vector, which facilitates in-frame construction of expression vectors.