Abstract: This invention particularly discloses an improved immunoassay method for the specific detection of anti-rubella IgM antibodies. This method employs rubella antigens comprising rubella E1 and E2 envelope glycoproteins substantially free of capsid protein. Use of this antigen composition reduces or eliminates nonspecific protein-protein interactions leading to false positive results. A sample diluent comprising urea can also be used in the process to further reduce the occurrence of nonspecific protein-protein interactions. A diagnostic kit to aid in the detection of anti-rubella IgM antibodies is also disclosed.
Type:
Grant
Filed:
October 29, 2003
Date of Patent:
March 29, 2005
Assignee:
Viral Antigens, Inc.
Inventors:
Preston H. Dorsett, Frances I. Byrd, Robert F. Devlin
Abstract: This invention particularly discloses an improved immunoassay method for the specific detection of anti-rubella IgM antibodies. This method employs rubella antigens comprising rubella E1 and E2 envelope glycoproteins substantially free of capsid protein. Use of this antigen composition reduces or eliminates nonspecific protein-protein interactions leading to false positive results. A sample diluent comprising urea can also be used in the process to further reduce the occurrence of nonspecific protein-protein interactions. A diagnostic kit to aid in the detection of anti-rubella IgM antibodies is also disclosed.
Type:
Grant
Filed:
May 7, 2001
Date of Patent:
December 30, 2003
Assignee:
Viral Antigens, Inc.
Inventors:
Preston H. Dorsett, Frances I. Byrd, Robert F. Devlin
Abstract: This invention particularly discloses an improved immunoassay method for the specific detection of anti-rubella IgM antibodies. This method employs rubella antigens comprising rubella E1 and E2 envelope glycoproteins substantially free of capsid protein. Use of this antigen composition reduces or eliminates nonspecific protein-protein interactions leading to false positive results. A sample diluent comprising urea can also be used in the process to further reduce the occurrence of nonspecific protein-protein interactions. A diagnostic kit to aid in the detection of anti-rubella IgM antibodies is also disclosed.
Type:
Application
Filed:
May 7, 2001
Publication date:
December 27, 2001
Applicant:
VIRAL ANTIGENS, INC.
Inventors:
Preston H. Dorsett, Frances I. Byrd, Robert F. Devlin