Patents Assigned to Vivione Biosciences, LLC
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Patent number: 9194868Abstract: In various embodiments, the present disclosure describes methods and systems for detecting microbes in a sample. The methods are generally applicable to quantifying the number of target bacteria in a sample counted from a detection region of a flow cytometer histogram. The detection methods can be employed in the presence of other microorganisms and other non-target microbe components to selectively quantify the amount of a target microbe. The methods are advantageous over those presently existing for testing of foodstuffs and diagnostic evaluation in their speed, accuracy and ease of use. Various swab collection devices and kits useful for practicing the present disclosure are also described herein.Type: GrantFiled: August 17, 2009Date of Patent: November 24, 2015Assignees: The United States of America, Vivione Biosciences, LLCInventors: Dan A. Buzatu, Jon G. Wilkes, Ted A. Moskal, Bill Nevius, Jason T. Taylor, Randal K. Tucker, Melinda Miller, Shawn Ramsanoop
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Publication number: 20150276573Abstract: In various embodiments, the present disclosure describes methods and systems for detecting microbes in a sample. The methods are generally applicable to quantifying the number of target bacteria in a sample counted from a detection region of a flow cytometer histogram. The detection methods can be employed in the presence of other microorganisms and other non-target microbe components to selectively quantify the amount of a target microbe. The methods are advantageous over those presently existing for testing of foodstuffs and diagnostic evaluation in their speed, accuracy and ease of use. Various swab collection devices and kits useful for practicing the present disclosure are also described herein.Type: ApplicationFiled: March 26, 2014Publication date: October 1, 2015Applicant: Vivione Biosciences, LLCInventors: Dan Buzatu, Jon Wilkes, Ted Moskal, Bill Nevius, Melinda Miller, Shawn Ramsaroop
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Publication number: 20150275264Abstract: In various embodiments, the present disclosure describes methods and systems for detecting microbes in a sample. The methods are generally applicable to quantifying the number of target bacteria in a sample counted from a detection region of a flow cytometer histogram. The detection methods can be employed in the presence of other microorganisms and other non-target microbe components to selectively quantify the amount of a target microbe. The methods are advantageous over those presently existing for testing of foodstuffs and diagnostic evaluation in their speed, accuracy and ease of use. Various swab collection devices and kits useful for practicing the present disclosure are also described herein.Type: ApplicationFiled: March 26, 2014Publication date: October 1, 2015Applicant: Vivione Biosciences, LLCInventors: Dan Buzatu, Jon Wilkes, Ted Moskal, Bill Nevius, Melinda Miller, Shawn Ramsaroop
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Publication number: 20150275263Abstract: In various embodiments, the present disclosure describes methods and systems for detecting microbes in a sample. The methods are generally applicable to quantifying the number of target bacteria in a sample counted from a detection region of a flow cytometer histogram. The detection methods can be employed in the presence of other microorganisms and other non-target microbe components to selectively quantify the amount of a target microbe. The methods are advantageous over those presently existing for testing of foodstuffs and diagnostic evaluation in their speed, accuracy and ease of use. Various swab collection devices and kits useful for practicing the present disclosure are also described herein.Type: ApplicationFiled: March 26, 2014Publication date: October 1, 2015Applicant: Vivione Biosciences, LLCInventors: Dan Buzatu, Jon Wilkes, Ted Moskal, Bill Nevius, Melinda Miller, Shawn Ramsaroop
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Patent number: 9103788Abstract: Methods for detecting one or more target bacteria in a test sample are provided. It is shown herein that photosensitizers combined with intense light exposure reduce fluorescing background due to non-bacterial particles. This permits detection of subsequently labeled target bacterial cells (e.g., using a fluorescently labeled antibody) against a largely black background. In particular examples, the methods include incubating the test sample in a growth medium that permits growth of bacteria present in the sample, contacting the sample with a photo-sensitizer; exposing the sample to light under conditions sufficient for the photo-sensitizer to photobleach contaminating non-bacterial particulates present in the sample. The bacteria can then be substantially separated from the sample, thereby generating an isolated bacterial sample. The method can also include contacting the isolated bacterial sample with a binding agent specific for the one or more target bacteria, and detecting the one or more target bacteria.Type: GrantFiled: November 30, 2012Date of Patent: August 11, 2015Assignees: THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES, VIVIONE BIOSCIENCES, LLCInventors: Jon G. Wilkes, Dan Buzatu, Randal Tucker, Thaddeus John Moskal, Jr.
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Publication number: 20130137119Abstract: Methods for detecting one or more target bacteria in a test sample are provided. It is shown herein that photosensitizers combined with intense light exposure reduce fluorescing background due to non-bacterial particles. This permits detection of subsequently labeled target bacterial cells (e.g., using a fluorescently labeled antibody) against a largely black background. In particular examples, the methods include incubating the test sample in a growth medium that permits growth of bacteria present in the sample, contacting the sample with a photo-sensitizer; exposing the sample to light under conditions sufficient for the photo-sensitizer to photobleach contaminating non-bacterial particulates present in the sample. The bacteria can then be substantially separated from the sample, thereby generating an isolated bacterial sample. The method can also include contacting the isolated bacterial sample with a binding agent specific for the one or more target bacteria, and detecting the one or more target bacteria.Type: ApplicationFiled: November 30, 2012Publication date: May 30, 2013Applicants: Vivione Biosciences, LLC, ServicesInventors: The United States of America, as represented by the Secretary, Department of Health and Human Services, Vivione Biosciences, LLC