Abstract: An enzyme-containing detergent composition for a presterilization treatment of medical instruments and equipment, consisting of the following components, percent by weight:anionic surfactants: 4.0-6.0sodium phosphate: 30.0-35.0sodium silicate: 20.0-25.0sodium carbonate: 19.0-22.0soap comprising sodium salts of fatty acids: 2.0-4.0enzymatic preparation: 0.5-2.0sodium sulphate: the balance,the enzymatic preparation having the following composition, percent by weight:alkaline protease: 30-60neutral protease: 27-45elastase: 0.01-5.00collagenase: 0.001-4.00leucinaminopeptidase: 0.0001-0.011carboxypeptidase: 0.04-0.15fibrinolytic enzyme: 0.002-1.500lipase: 0.5-2.0amylase: the balance.