Abstract: The invention relates to a resistance gene and functional homologues or fragments thereof isolated from S. chacoense, S. berthaultii, S. sucrense or S. tarijense. Moreover, the invention relates to the use of said resistance gene, for example the use of said resistance gene in a method to increase or confer at least partial resistance in a plant to an oomycete infection. The invention provides an isolated or recombinant nucleic acid sequence comprising a nucleic acid sequence encoding one of the amino acid sequences of FIG. 4 or a functional fragment or a functional homologue thereof such as those presented in FIG. 13.

Abstract: The invention relates to a resistance gene and functional homologues or fragments thereof isolated from S. chacoense, S. berthaultii, S. sucrense or S. tarijense. More over, the invention relates to the use of said resistance gene, for example the use of said resistance gene in a method to increase or confer at least partial resistance in a plant to an oomycete infection. The invention provides an isolated or recombinant nucleic acid sequence comprising a nucleic acid sequence encoding one of the amino acid sequences of FIG 4 or a functional fragment or a functional homologue thereof such as those presented in FIG. 13.

Abstract: A process for the crystallisation of a water-soluble compound is disclosed. The process comprises (a) providing, in a crystallisation vessel, a solution of the water-soluble compound in a mixture of water and a solvent in which the water-soluble compound has a lower solubility than in water; (b) passing vapour phase of the mixture through a sorption zone containing a water vapour sorbent to selectively adsorb water from the vapour phase; (c) recycling a part of the vapour phase to the crystallisation vessel or withdrawing vapour phase depleted in water from the process and adding solvent to the crystallisation vessel; (d) allowing solid crystals of the water-soluble compound to precipitate from the solution; and (e) discharging precipitated solid crystals of the water-soluble compound from the crystallisation vessel and discharging a solution of non-crystallised water-soluble compound in water-solvent mixture from the crystallisation vessel.

Abstract: A process for the crystallization of a water-soluble compound from a solution of water and a solvent is provided. A process for the manufacture of crystalline sucrose from sugar palm juice or sucrose-containing biomass comprising such crystallization process is also disclosed.

Abstract: The present invention relates to medicine, particularly immunology and gastroenterology. Specifically, it relates to probiotic bacteria and extracts thereof for therapeutic use for the treatment of inflammatory disorders such as inflammatory bowel disease. Provided is a composition comprising as active ingredient Faecalibacterium prausnitzii strain HTF-F (DSM 26943) or an extract thereof comprising extracellular polymeric matrix (EPM), and an acceptable carrier, diluent or excipient. Also provided is an anti-inflammatory composition comprising EPM extracted from F. prausnitzii strain HTF-F, and a method for preparing the same.

Abstract: The present invention relates to a method for determining the presence of a cyst nematode in a sample comprising the steps of: providing a pair of bidirectional oligonucleotide primers or an oligonucleotide probe that hybridizes specifically, under stringent hybridization conditions, to a nucleic acid sequence encoding the SSU rRNA or LSU rRNA, or the complement or transcript thereof, of a sub genus-cluster of nematodes, said subgenus-cluster comprising cyst nematodes belonging to at least one species of nematode, and wherein said primers or probe do not hybridize to a nucleic acid sequence encoding the LSU rRNA, or the complement or transcript thereof, of cyst nematodes not part of said subgenus-cluster of cyst nematodes; providing a sample in which the presence of the cyst nematode is to be detected, and performing a nucleic acid detection assay on said sample using said pair of bidirectional oligonucleotide primers or said oligonucleotide probe.

Abstract: The invention relates to a Nuclear Magnetic Resonance (NMR) spectroscopy device adapted for carrying out 1D and nD homo- and heteronuclear NMR spectroscopy measurements of a plurality of nuclei, comprising an RF coil adapted to transmit RF to and/or receive RF from a measuring volume, wherein the RF coil forms part of a non-tuned radiofrequency circuit. The invention further relates to a method of NMR data acquisition, a method of manufacturing a NMR spectroscopy device and a NMR-device holder.

Abstract: Described is the use of Eubacterium hallii et rel. and/or Alcaligenes faecalis et rel., as well as pharmaceutical, food, or feed compositions comprising these bacteria as a medicament, in particular, for preventing and/or treating insulin resistance and/or insulin resistance-related complications such as metabolic syndrome, dyslipidemia and type 2 diabetes mellitus, as well as insulin resistance in endocrine diseases (e.g., obese subjects with type 1 diabetes mellitus, Cushing's disease and lipodystrophy syndromes. Also described is a method for preventing and/or treating insulin resistance and/or insulin resistance-related complications such as dyslipidemia and type 2 diabetes mellitus as well as insulin resistance in endocrine diseases (e.g., obese subjects with type 1 diabetes mellitus, Cushing's disease and lipodystrophy syndromes) in a subject in need thereof, the method comprising the step of increasing the level of Eubacterium hallii et rel. and/or Alcaligenes faecalis et rel. in the small intestine.

Abstract: Molecular markers are described for determining the presence or absence of a gene conferring resistance to tomato yellow leaf curl virus from S. chilense (Ty-1) in a host plant. Also described, are methods for producing a host plant comprising a gene conferring resistance to tomato yellow leaf curl virus from S. chilense (Ty-1), including the analysis of the presence or absence of the molecular markers. A plant, and parts thereof, obtained by such a method are also described.

Abstract: The invention relates to a resistance gene and functional homologs or fragments thereof isolated from Solanum bulbocastanum. Moreover, the invention relates to the use of this resistance gene, for example, in a method to increase or confer at least partial resistance in a plant to an oomycete infection.

Abstract: The present invention relates to the field of plant biotechnology. More in particular, the present invention relates to methods for increasing the resistance of a plant or part thereof that is susceptible to infection with a pathogen comprising an ortholog of the Avr4 protein of Cladosporium fulvum, wherein said plant is not a tomato or tobacco plant. The invention also relates to methods for screening the resistance of a plant or a part thereof to at least one pathogen, wherein said pathogen is not Cladosporium fulvum, wherein said plant is not a tomato plant. The invention further relates to the use of such methods.

Abstract: The invention relates to a resistance gene and functional homologues or fragments thereof isolated from S. chacoense, S. berthaultii, S. sucrense or S. tarijense. More over, the invention relates to the use of said resistance gene, for example the use of said resistance gene in a method to increase or confer at least partial resistance in a plant to an oomycete infection. The invention provides an isolated or recombinant nucleic acid sequence comprising a nucleic acid sequence encoding one of the amino acid sequences of FIG. 4 or a functional fragment or a functional homologue thereof such as those presented in FIG. 13.

Abstract: The invention relates to a method to change the capacity of a plant for adaptation to changes in the zinc concentration in the environment, especially the soil, comprising providing said plant with a nucleotide according to SEQ ID NO:1 or SEQ ID NO: 2 or an ortholog thereof. Such a method is especially useful to decrease Zn deficiency or to (hyper)accumulate Zn in plants for bioremediation or for biofortification. Transgenic plants for these proteins are also disclosed.

Abstract: Disclosed is a method for the combined synthesis of at least two vinylic monomers, at least one of which being an acrylic compound, comprising subjecting a monoconjugated alkene-1-carboxylic compound to reaction with a C2-C4 alkene under conditions of olefin cross-metathesis. The invention is particularly useful for extracting value from protein side streams. Upon protein hydrolysis, suitable amino acids (preferably phenylalanine or tyrosine) are subjected to reductive amination so as to form the corresponding alkene-1-carboxylic acid. Preferably after esterification and separation, this is used in cross-metathesis for the concomitant production of styrene resp. hydroxy styrene, and acrylates. The invention is applicable more widely, to the synthesis of olefins on the basis of carbohydrates, naturally occurring phenolic components, natural protein resources, or amino acids obtained from fermentations.

Abstract: The present invention relates to methods for identifying one or more bioactive genes, comprising: (a) introducing an expressible genomic DNA library derived from a first organism or a group of first organisms into a second organism, wherein said genomic DNA library is comprised in a copy inducible vector in said second organism; (b) growing said multitude of clones of said second organism at a low copy number of said vector and at high copy number of said vector; (c) identifying one or more clones of said second organism wherein said identification comprises identifying altered growth characteristic; and (d) identifying in the one or more clones of said second organism identified in step (c) one or more genes of said first organism or said group of first organisms providing the altered growth characteristic, thereby identifying the one or more bioactive genes.

Abstract: The invention relates to a resistance gene and functional homologues or fragments thereof isolated from S. bulbocastanum. More over, the invention relates to the use of said resistance gene, for example the use of said resistance gene in a method to increase or confer at least partial resistance in a plant to an oomycte infection. The invention provides an isolated or recombinant nucleic acid sequence comprising a nucleic acid sequence encoding one of the amino acid sequences of FIG. 6 or a functional fragment or a functional homologue thereof.

Abstract: The present invention relates to a method of testing a sample for the presence of a Meloidogyne nematode, said method comprising the step of analyzing the nucleic acid in said sample for the presence of an rRNA gene of a Meloidogyne nematode, or the transcription product thereof, or a fragment thereof, comprising at least one single nucleotide polymorphism (SNP) and/or at least one oligonucleotide polymorphism (OP) that is essentially unique on phylum level to a specific group of Meloidogyne nematode species of which said Meloidogyne nematode is a member, wherein said step of analyzing said nucleic acid involves a nucleic acid hybridization assay, and wherein said group comprises (a) the species M. chitwoodi, M. fallax and M. minor; (b) the species M. naasi, M. oryzae and M. graminicola; (c) the species M. hapla, M. microtyla, M. ardenensis, M. maritime and M. duytsi; and/or (d) the species M. incognita, M. arenaria and M. jaυanica.

Abstract: The present invention relates to a method for determining the presence of a cyst nematode in a sample comprising the steps of : providing a pair of bidirectional oligonucleotide primers or an oligonucleotide probe that hybridizes specifically, under stringent hybridization conditions, to a nucleic acid sequence encoding the SSU rRNA or LSU rRNA, or the complement or transcript thereof, of a sub genus-cluster of nematodes, said subgenus-cluster comprising cyst nematodes belonging to at least one species of nematode, and wherein said primers or probe do not hybridize to a nucleic acid sequence encoding the LSU rRNA, or the complement or transcript thereof, of cyst nematodes not part of said subgenus-cluster of cyst nematodes; providing a sample in which the presence of the cyst nematode is to be detected, and performing a nucleic acid detection assay on said sample using said pair of bidirectional oligonucleotide primers or said oligonucleotide probe.

Abstract: The invention provides a method for modifying a starch producing plant and a method for producing starch having at least one altered characteristic. The invention particularly provides a method for producing starch comprising introducing a nucleic acid comprising an amylosucrase encoding sequence functionally linked to a granule-targeting sequence and functionally linked to a promoter sequence active in a starch storage organ of said plant into suitable plant material of said plant and producing a plant from said material, further comprising allowing said plant to grow and harvesting starch from said plant. The invention further provides starch having an altered characteristic and plants and parts thereof and products comprising such starch. Furthermore, the invention provides fusion proteins and cells comprising a fusion protein.

Abstract: The invention relates to a method for expressing a desired protein or polypeptide in a plant, in which the protein or polypeptide is expressed as a fusion with at least one starch binding domain. The plant is preferably a plant that contains or produces starch or starch granules in at least one of its parts, such as potato, sweet potato, cassava, pea, taro, sago, yam, banana and/or cereals such as rice, maize, wheat and barley. The protein or polypeptide can be an enzyme, in particular an enzyme that can convert, modify, alter, degrade or otherwise influence starch (granules); or can be a receptor or a structural protein. The invention further relates to the fusions thus obtained, to genetic constructs that encode the above fusions and to plants transformed with said constructs. The method of the invention can in particular be used to provide modified starches and/or to provide complexes of starch (granules) and the above fusions.