Abstract: The invention relates to a punching tool for taking a biological sample from a filter using punching, the punching tool comprising a punch and a die provided with a punching channel for conveying the sample from the upper surface of the die towards the lower end of the die that includes a guidance means of the sample comprising a guidance channel arranged as an extension of the punching channel for receiving the sample from the punching channel, the diameter of the guidance channel is 1,02 to 2× the diameter of the punch and is greater than the diameter of the punching channel, and at the connecting point with the punching channel the guidance channel forms a shoulder and broadens towards the free end of the guidance means.