Abstract: cDNAs encoding E-&agr;-bisabolene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase from Grand Fir (Abies grandis) have been isolated and sequenced, and the corresponding amino acid sequences have been determined. Accordingly, isolated DNA sequences (SEQ ID No:12 and SEQ ID No:19 and SEQ ID No:23) are provided which code for the expression of E-&agr;-bisabolene synthase (SEQ ID No:13), &dgr;-selinene synthase (SEQ ID No:20) and &ggr;-humulene synthase (SEQ ID No: 24), respectively, from Grand Fir (Abies grandis). In other aspects, replicable recombinant cloning vehicles are provided which code for E-&agr;-bisabolene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase, or for a base sequence sufficiently complementary to at least a portion of E-&agr;-bisabolene synthase, &dgr;-selinene synthase or &ggr;-humulene synthase DNA or RNA to enable hybridization therewith.
Type:
Grant
Filed:
January 20, 1999
Date of Patent:
July 24, 2001
Assignee:
Washington State University Foundation
Inventors:
Rodney Bruce Croteau, Jörg Bohlmann, Reinhard Jetter, John E. Crock, Christopher L. Steele
Abstract: A new and distinctive variety of an Malus domestica ‘apple’ tree, named ‘WA 2’, that is distinguished by its attractive blush, outstanding texture being firm, crisp and juicy, and the presence of the homozygous genes for ethylene production ACS1-2/2 and ACO1-2/2 which contribute to maintaining the fruit quality over long periods of storage.