Abstract: The present invention provides heterologous complementation systems and methods of using the systems to detect molecular interactions. In particular, the heterologous complementation systems comprise polypeptide fragments derived from heterologous polypeptides. If a molecular interaction occurs, then the heterologous polypeptide fragments are able to associate and produce a detectable signal.

Abstract: The present invention relates to methods of treating pneumonic plague. More specifically, the present invention relates to methods of using plasmin or Pla inhibitors to treat pneumonic plague.

Abstract: The present invention relates to methods of diagnosing, monitoring, and assessing treatment effects for neurological and neurodegenerative diseases and disorders, such as Alzheimer's Disease, early in the course of clinical disease or prior to the onset of brain damage and clinical symptoms. Methods of measuring the in vivo metabolism of biomolecules produced in the CNS in a subject are provided.

Abstract: A system for verifying the authenticity of data contained in a magnetic medium using the remanent noise characteristics of the magnetic medium. In one embodiment, the invention includes a sensing unit configured to generate a signal indicative of the sensed magnetic field and an amplifier. The amplifier is in communication with the sensing unit, is configured to amplify signals having amplitudes less than a threshold by at least a first factor and configured to amplify signals having amplitudes greater than the threshold by at most a second factor. The first factor is greater than the second factor.

Abstract: The present invention provides compositions and methods for inhibiting protozoan growth comprising a synergistic combination of lipid synthesis inhibitors. In addition, the invention provides compositions and methods that are useful for the treatment of protozoan infections and the identification of potential new drugs for the treatment of protozoan infections.

Abstract: The present invention provides methods for diagnosing and/or treating subjects that either have or are at risk for developing acute coronary syndrome. In addition, the invention provides isolated nucleotide sequences and arrays comprising the nucleotide sequences that may be used for treatment and/or diagnosis of subjects that either have or are at risk for developing acute coronary syndrome.

Abstract: The invention provides methods for identifying a compound that inhibits cytochrome c synthesis. This invention further provides a method for the high throughput screening of compounds that inhibit cytochrome c synthesis.

Abstract: Generally speaking, the present invention relates to variant helicases that lack structural autoinhibition of helicase activity. In particular, the invention provides a composition and a kit comprising a helicase that lacks structural autoinhibition, and a method of unwinding a double helix comprising contacting the double helix with a helicase that lacks structural autoinhibition.

Abstract: A method for determination of at least one of the triglyceride molecular species in a biological sample comprising the subjecting sample to lipid extraction to obtain a lipid extract and subjecting the resulting lipid extract to 2D electrospray ionization tandem mass spectrometry (ESI/MS/MS) with neutral loss scanning of all naturally occurring aliphatic chains and contour analysis of 2D intercept peaks. A method for determination of tricylglyceride content and/or TG molecular species directly from a lipid extract of a biological sample comprising subjecting said lipid extract to electrospray ionization tandem mass spectrometry.

Abstract: The present invention provides a novel lymphocyte inhibitory receptor termed BTLA which is expressed on both T and B cells, and identifies B7 family member B7x as interacting with BTLA to attenuate lymphocyte activity. Methods and compositions for modulating BTLA-mediated negative signaling and interfering with the interaction of BTLA and B7x for therapeutic, diagnostic and research purposes are also provided.

Abstract: A micro-radiation therapy apparatus includes an isotope-based micro-radiotherapy brachytherapy small animal irradiator useful for radiating a biological system, the irradiator having an external radiation source proximate the biological system comprising a collimated radiation beam. A method of effectively irradiating at least one cell in a biological system includes applying micro-radiation from an isotope-based micro-radiation small animal irradiator, the irradiator having an external radiation source proximate the biological system including a collimated radiation beam to a target cell of the biological system.

Abstract: A method for determination of at least one of the lipid species in a biological sample comprising subjecting the sample to lipid extraction to obtain a lipid extract and subjecting the resulting lipid extract to multidimensional electrospray ionization mass spectrometry using either precursor ion or neutral loss scanning (or both) of all naturally occurring aliphatic chains, lipid fragments and precursor ions leading to observed fragments to generate a multidimensional matrix whose contour densities provides structural and quantitative information directly without chromatography. A method for determination of lipid content and/or lipid molecular species composition and quantity directly from lipid extracts of a biological sample comprising subjecting said lipid extract to electrospray ionization multidimensional mass spectrometry by comparisons to standards and algorithms described herein.

Abstract: The invention generally provides a biomarker for sleepiness, a method for detecting sleepiness, and a method of identifying nucleic acids associated with sleepiness.

Abstract: The present invention relates to an orthotic device wherein the patient's feet are maintained at a desired angle while allowing the patient to move in an otherwise normal manner and preventing blister formation. The orthotic device generally comprises an orthotic splint assembly, two ankle foot orthosis and two custom molded inserts secured to the ankle foot orthosis.

Abstract: The invention provides methods for treating non-age related hearing impairments, especially sensorineural and neural hearing loss, in a subject in need of such treatment.

Abstract: A method for evaluating a compound to determine the relative or absolute therapeutic capability of a compound to pharmacologically inhibit ischemia induced activation of iPLA2? in an intact myocardium which comprises treating intact myocardium with a compound, inducing ischemia in the myocardium tissue and determining if there has been a change in expression of iPLA2?, its activity or in the iPLA2? regulating network and determining if there has been a change then that the compound is an anti-arrhythmia or myocardial salvage drug.

Abstract: Glycosylated or nonglycosylated molecules of the formula ?1-(linker1)n1-?2-(linker2)n2-?3-(linker3)n3-?;??(1) ?1-(linker1)n1-?2-(linker2)n2-?-(linker3)n3-?3 ;??(2) ?1-(linker1)n1-?-(linker2)n2-?2-(linker3)n3-?3 ;??(3) and ?-(linker1)n1-?1-(linker2)n2-?2-(linker3)n3-?3 ??(4) wherein ? is the ? subunit of a vertebrate glycoprotein hormone or a variant thereof; each ? is independently a glycoprotein ? subunit or a variant thereof, each “linker” is a hydrophilic, flexible spacer equivalent to a peptide containing 1–100 amino acid residues; and each n is a 0 or 1; said compound optionally comprising one or more additional ?x(linkerx)nx and/or one or more additional ? subunits are useful in protocols to enhance fertility in humans and in animals.

Abstract: An improved method for charging ultrafine particles in coronas (e.g., unipolar or bipolar corona discharges) by exposing the particles to X-ray irradiation. Experimental tests have verified that positive corona in the presence of X-ray irradiation results in maximum or optimum charging efficiency, followed by a negative corona in the presence of X-ray irradiation, X-ray radiation only (without corona), negative corona only (without X-ray irradiation), and finally positive corona only (without X-ray irradiation). This method and system is particularly well suited for use with bioaerosol particles wherein exposure to the corona discharge and X-ray irradiation serves to both capture and inactivate the bioaerosol particles using a single device.

Abstract: Novel pyridinones and their derivatives which are effective in treating or preventing Gram-negative bacterial infections are provided. The pyridinones are stable and easily derivatized; the methods by which these derivatizations occur is described. Two regioselective and functional group tolerant methods for the synthesis of the novel pyridinones are also provided. One such synthetic method involves reacting an imine and a Meldrum's acid derivative in solution. The other synthetic method is a solid phase synthesis of the pyridinones in which an imine is prepared bound to a solid support and a Meldrum's acid derivative is reacted with the imine. Novel imine intermediates useful in the solid phase and solution methods of synthesizing the pyridionones are also described.

Abstract: The present invention provides transgenic non-human mammals and cells having one or more structurally and functionally disrupted NIK alleles. In addition, the present invention provides methods for making such transgenic mammals and cells, and methods for determining the effect of a compound on an animal or cell that lacks NIK function. The present invention also provides methods for identifying compounds useful in the inhibition of osteoclastogenesis in a mammal, for example to treat osteoporosis or other conditions. Also provided are methods of modulating the extent of osteoclastogenesis in a cell or a mammal using NIK modulators.