Abstract: The present disclosure describes methods for analyzing mRNA poly(A) tail sequences with a high resolution to determine poly(A) tail length and heterogeneity. The method involves digesting an mRNA molecule to liberate a poly(A) tail, preparing a chromatographic sample comprising the mRNA poly(A) tails, preparing a second chromatographic sample comprising a reference sequence comprising a mRNA poly(A) tails having a predetermined length, separating the first and second samples by a chromatography method, which result in one or more chromatograms, and determining a sequence length of the mRNA poly(A) tails by comparing the chromatograms of the first and second samples. Chromatography methods for analyzing the poly(A) tail may include ultraviolet size-exclusion chromatography (SEC UV), ultraviolet ion-pair reversed-phase liquid chromatography (IP RP LC UV), ultra high performance liquid chromatography (UPHLC), and combinations thereof.

Abstract: Provided herein is a multi-component chromatographic material and use thereof for reversed-phase liquid chromatography. The multi-component chromatographic materials provided herein comprise a chromatographic core having an exterior surface; and at least two different hydrophobic ligands covalently bound to the exterior surface with a total surface coverage less than 2.0 ?mol/m2. The multi-component chromatographic materials of the present technology are beneficial for reversed-phase liquid chromatography using highly aqueous mobile phases. For example, chromatographic materials described herein allow mitigating or preventing significant retention loss of reversed-phase liquid chromatography columns after flow interruption.

Abstract: Provided herein is a tubular protein capture device including a continuous tubular body having an inner surface and an outer surface and methods of using thereof for cell clarification purposes. A protein selected from protein A, protein G, protein L or combination thereof is attached to the inner surface of the tubular protein capture device described herein. The devices and methods provided herein are useful for improving the efficiency and/or capacity of cell clarification to separate proteins. In some embodiments, the continuous tubular body of the present technology has a helical shape. In other embodiments, the continuous tubular body of the present technology includes a plurality of tubes aligned within a hollow enclosure.

Abstract: A liquid chromatography system includes a solvent delivery system, a sample manager including a sample delivery system in fluidic communication with the solvent delivery system, the sample delivery system configured to inject a sample from a sample-vial into a chromatographic flow stream, a liquid chromatography column located downstream from the sample delivery system, and a detector located downstream from the liquid chromatography column. The sample delivery system further includes a first needle drive including a first sample needle configured to extract the sample from the sample-vial and deliver the sample to the liquid chromatography column, and a first syringe in communication with the first sample needle configured to meter extraction of the sample from the sample-vial. The sample manager further includes a sample automation system that includes a second needle drive including a second sample needle configured to add a volume of reagent to the sample-vial.

Abstract: The present disclosure discusses a method of separating a sample (e.g., pharmaceutical drug, genotoxic impurity, biomarker, and/or biological metabolite) including coating a metallic flow path of a chromatographic system; injecting the sample into the chromatographic system; flowing the sample through the chromatographic system; separating the sample; and analyzing the separated sample using mass spectroscopy. In some examples, the coating applied to the surfaces defining the flow path is non-binding with respect to the sample—and the separated sample. Consequently, the sample does not bind to the low-binding surface of the coating of the flow path. The applied coating can increase the chromatographic peak area for the sample of the chromatographic system.

Abstract: Described is a method for regulating a volume of liquid delivered by a peristaltic pump and a peristaltic pump system that can be used to perform the method. The method includes sensing an ambient temperature of the peristaltic pump. The peristaltic pump includes a pump motor. At least one of a motor speed and a motor operation duration is determined from the sensed temperature, a selected volume of liquid to be delivered and a predetermined correspondence of the motor speed to ambient temperature. The pump motor is operated at the determined motor speed or for the determined motor operation duration to deliver the selected volume of liquid from the peristaltic pump.

Abstract: The present invention provides novel chromatographic materials, e.g., for chromatographic separations, processes for their preparation and separations devices containing the chromatographic materials. The preparation of the inorganic/organic hybrid materials of the invention wherein a surrounding material is condensed on a superficially porous hybrid core material will allow for families of different hybrid packing materials to be prepared from a single core hybrid material. Differences in hydrophobicity, ion-exchange capacity, chemical stability, surface charge or silanol activity of the surrounding material may be used for unique chromatographic separations of small molecules, carbohydrates, antibodies, whole proteins, peptides, and/or DNA.

Abstract: The present disclosure discusses a method of separating and/or purifying polynucleotides. The method includes injecting a sample into cartridge that is packed with a porous sorbent between opposing frits. Physical properties of the cartridge along with the use of hydrophilic fits allows efficient desalting of biopolymers using a vacuum to pull the sample through the cartridge.

Abstract: Provided herein is a method and system for PFAS analysis using a liquid chromatography technique that employ a first column (e.g., an isolator column) and a second column (e.g., an analytical column), wherein at least one of the first column, the second column, or both the first and second columns include mixed mode with anion exchange surface chemistry. The devices and methods provided herein are useful for improving the efficiency and/or sensitivity of systems implementing liquid chromatography for PFAS analysis. The methods of the present disclosure are particularly beneficial for trace level (e.g., ppm level or lower) analysis of PFAS. Further, the methods of the present disclosure allow improved separation of short-chain and ultrashort-chain PFAS.

Abstract: Described is a multi-channel fluidic device that includes a diffusion-bonded body having a device surface and a plurality of fluid channels. Each fluid channel includes a channel segment defined in a plane that is parallel to the device surface and parallel to each of the planes of the other channel segments. The plane of each channel segment is at a depth below the device surface that is different from the depth below the device surface for the other planes. Each channel segment may have a volume equal to the volume of each of the other channel segments. One of the fluid channels may include a plurality of channel segments serially connected to each other and each defined in a plane that is different from the planes of the other channel segments.

Abstract: This disclosure pertains to performance enhancing conditioning and storage solvents containing low levels of buffer and salt for reproducible and improved size exclusion chromatography (SEC). In some embodiments, the present disclosure pertains to storage solvents for protein-based size exclusion chromatography that include water, a water-miscible organic solvent, a phosphate salt, and a neutral salt. In some embodiments, the present disclosure pertains to columns for protein-based size exclusion chromatography that include porous particles and such a storage solvent.

Abstract: The present technology relates to a method and instrument for classifying a sample. The method collects raw data from an analytical instrument (e.g., LC-MS), quantifies consistency parameters from the raw chromatographic data (e.g., peak detection) by assigning criteria and determining probability ratios, and constructs a learning model quantitation step by weighing the presence, absence, or modulation of one or more factors of the one or more samples against a decision criterion for positivity. The method can then apply the learning model to an unknown sample to detect the presence, absence, or modulation of one or more factors in the unknown sample such as the presence, absence, or modulation of a disease state or multiple disease states.

Abstract: A vial cap for a sample vial includes a cylindrical cap body defining a first circular opening at a first end. The vial cap also includes a cap lid extending radially inward from a second end of the cylindrical cap body. The cap lid defines a second circular opening. The vial cap also includes a septum located within the cylindrical cap body and in contact with the cap lid. The septum spans the second circular opening defined by the cap lid portion. The vial cap also includes a number of flexible threads extending from an internal surface of the cylindrical cap body.

Abstract: A method is provided for authenticating a botanical. The method includes identifying at least one marker compound of the botanical. A sample comprising the botanical is injected into a chromatography system that includes a mobile phase delivery module, an autosampler, a chromatography column, a chromatography column manager, and at least one detector. The method also includes extracting a chromatogram of the at least one marker compound and extracting a spectrum at a chromatographic peak retention time of the at least one marker compound. The method also includes quantifying at least one peak of the extracted chromatogram and quantifying at least one band of the extracted spectrum. The quantified at least one peak of the extracted chromatogram and the quantified at least one band of the extracted spectrum are compared to a set of authentication criteria to determine authenticity of the botanical.

Abstract: Disclosed is a gradient proportioning valve for liquid chromatography that includes a plurality of inlet ports configured to receive a plurality of fluids, a manifold connected to each of the plurality of inlet ports configured to mix the plurality of fluids in a controlled manner to provide a fluid composition, the manifold including a plurality of conduits internal to the manifold, each of the plurality of conduits receiving fluid through a respective one of the plurality of inlet ports, an actuation mechanism having a piston located within a bored structure surrounding the piston, the actuation mechanism configured to open and close at least one of the plurality of conduits in a controlled manner where the piston and the bored structure have a tight tolerance configured to create a fluid tight seal, and a common outlet port configured to receive the fluid composition.

Abstract: The present disclosure pertains to non-porous composite particles that are non-porous, polymer-based particles. In various embodiments, a non-porous polymer core is surface modified. In various embodiments, a non-porous hybrid organic-inorganic material is in contact with the modified surface of the core, and a bonding material is in contact with the non-porous hybrid organic-inorganic material. The present disclosure pertains to chromatographic separation devices that comprise such non-porous composite particles.

Abstract: The present disclosure relates to devices and methods for preparing a filtered solution from slurry for lateral flow testing of analytes of interest in agricultural or environmental samples. A porous frit is located adjacent to an outlet of a vessel containing slurry, and positive pressure is applied to the volume enclosed by a vessel body of the vessel to cause the slurry to pass through the porous frit and become a filtered solution that exits the outlet of the vessel. Devices and methods described herein allow more rapid, cleaner, and inexpensive production of filtered samples than conventional methods.

Abstract: The present disclosure pertains to composite particles with polymer-based cores, which eliminate the high pH failure mechanism of silica-based core-shell silica particles. In various embodiments, a non-porous polymer core is surface modified. In various embodiments, a non-porous hybrid organic-inorganic material is in contact with the modified surface of the core, and a porous inorganic material is in contact with the non-porous hybrid organic-inorganic material. The present disclosure pertains to chromatographic separation devices that comprise such composite particles.

Abstract: This present disclosure relates to engineered protease enzymes, including trypsin, Lys-C and Asp-N proteases, that have enhanced autolysis resistance. Also disclosed herein are methods of using such engineered enzymes for improving detection of target analyte proteins in an analytical assay.

Abstract: The present disclosure pertains to core-shell particles that are superficially porous, polymer-based, and include organic-inorganic materials. In various embodiments, a non-porous polymer core is surface modified. In various embodiments, a non-porous hybrid organic-inorganic material is in contact with the modified surface of the core, and a porous hybrid organic-inorganic material is in contact with the non-porous hybrid organic-inorganic material. The present disclosure pertains to chromatographic separation devices that comprise such core-shell particles.