Abstract: The present disclosure is directed to liquid chromatography columns and methods utilizing a stationary phase sorbent having two or more gradient factors. Each of the two or more gradient factors varies in a progressive manner along a length of the stationary phase sorbent in a direction from an inlet to an outlet of the column (i.e., along a length of the column). As a result of including these new continuous stationary phase gradients, new selectivities allowing for separation and analysis of complex samples including large biomolecules is achievable.
Abstract: The present disclosure discusses a method of separating and/or purifying polynucleotides. The method includes injecting a sample into a chromatographic column that is packed with a porous sorbent having a pore size that substantially excludes the polynucleotides from the sorbent. This restricted access to the sorbent allows separation of large polynucleotides from each other and from smaller molecular weight impurities.
Type:
Application
Filed:
May 8, 2023
Publication date:
November 16, 2023
Applicant:
Waters Technologies Corporation
Inventors:
Matthew A. Lauber, Catalin Doneanu, Weibin Chen, Ying Qing Yu, Jennifer M. Nguyen, Fabrice Gritti, Martin Gilar
Abstract: The present disclosure relates to a streamlined, complete workflow for the qualitative and the quantitative analysis of conjugated peptides from antibody-drug conjugate (ADC) compounds.
Type:
Grant
Filed:
March 2, 2017
Date of Patent:
October 31, 2023
Assignee:
Waters Technologies Corporation
Inventors:
Liuxi Chen, Weibin Chen, Steven Cubbedge
Abstract: A clamp assembly includes a rail extending along the length and configured to receive a first fluidic assembly, and a carriage movably attachable to the rail such that the carriage moves along the rail, the carriage configured to receive a second fluidic assembly, the carriage including an actuator and a stop mechanism. The stop mechanism is configured to selectively prevent and allow movement of the carriage relative to the rail. The stop mechanism is configured to be independently operable from the actuator assembly, and the actuator is configured: to move a chromatography column received by the clamp assembly relative to the rail to create a first fluid tight seal between the chromatography column and the first fluidic assembly, and move the second fluidic assembly relative to the carriage body to create a second fluid tight seal between the second fluidic assembly and the chromatography column.
Type:
Grant
Filed:
April 26, 2021
Date of Patent:
October 24, 2023
Assignee:
WATERS TECHNOLOGIES CORPORATION
Inventors:
Wade P. Leveille, Joseph D. Michienzi, Joseph D. Antocci, Jeffrey Musacchio
Abstract: A sprayer assembly for an ion source is disclosed. The sprayer includes a capillary having an outlet, a sheath for the capillary, and an elastic member. The sheath can move relative to the capillary between a first position in which the sheath covers the outlet of the capillary and a second position in which the outlet of the capillary is exposed. When the sheath moves from the first position to or towards the second position, the elastic member provides a restoring force that acts to restore the position of the sheath to or towards the first position.
Type:
Grant
Filed:
January 22, 2021
Date of Patent:
October 24, 2023
Assignee:
Waters Technologies Corporation
Inventors:
Wade P. Leveille, Joseph D. Michienzi, Jeffrey Musacchio, Emrys Jones, Steven Derek Pringle, Stephen Hattan, Gregory T. Roman
Abstract: A heat flow rate measurement method for use with a differential scanning calorimeter sensor is provided. The method includes calculating a heat exchange between a plurality of sample containers and a reference container placed on a plurality of sample calorimeter units and a reference calorimeter unit, respectively, and determining a heat flow rate of samples within the sample containers using the calculated heat exchange between the plurality of sample containers and the reference container. A multiple sample differential scanning calorimeter sensor and calorimeter system are also provided.
Abstract: Disclosed herein are solvent reservoir systems for steady flow delivery and simultaneous monitoring of solvent level and solvent density within the solvent reservoir systems and methods for monitoring the solvent reservoir systems and providing feedback to a user or adjusting the systems in response to the monitored characteristics.
Abstract: A fitting for fluidic coupling in a chromatography system includes a compression screw including an axial bore, a threaded portion, and a drive end, a tube assembly including a tube sleeve and an inner tube disposed through the sleeve, the tube sleeve and the inner tube each extending to an endface of the tube assembly, the tube sleeve including an outer surface, a seal body extending between a first endface and a second endface, the first endface abutting the endface of the tube assembly, the seal body including an outer surface, and a collar secured to the outer surface of the tube sleeve and the outer surface of the seal body. Other Fittings, Methods of manufacture of fittings, and methods of fluidic coupling are further disclosed.
Type:
Grant
Filed:
August 12, 2020
Date of Patent:
October 10, 2023
Assignee:
WATERS TECHNOLOGIES CORPORATION
Inventors:
Mark W. Moeller, William B. Clark, Charles T. Murphy
Abstract: Described is a metering pump that may be used to meter volumes of sample in a chromatography system. The metering pump has a modular configuration that allows for separation of a head pod from a drive assembly for easy serviceability. The head pod includes a pump head, cartridge housing, seal wash housing and plunger. The drive assembly can be implemented in an inline drive configuration. Alternatively, the drive assembly can be implemented in an offset drive configuration in which a stepper motor is displaced laterally from other drive assembly components but is coupled through a belt and pulley system.
Type:
Grant
Filed:
March 25, 2021
Date of Patent:
October 3, 2023
Assignee:
WATERS TECHNOLOGIES CORPORATION
Inventors:
Joshua A. Burnett, Rose Solow, David A. Simpson, Peter Osswald, John M. Auclair, Michael LeBeau
Abstract: The present invention provides novel chromatographic materials, e.g., for chromatographic separations, processes for its preparation and separations devices containing the chromatographic material; separations devices, chromatographic columns and kits comprising the same; and methods for the preparation thereof. The chromatographic materials of the invention are chromatographic materials comprising having a narrow particle size distribution.
Type:
Application
Filed:
May 3, 2023
Publication date:
September 21, 2023
Applicant:
Waters Technologies Corporation
Inventors:
Nicole L. Lawrence, Daniel P. Walsh, Jacob Nathan Fairchild, Kevin Daniel Wyndham
Abstract: A method of capturing human immunoglobulin Fc domains in a biofluid sample is provided. The method includes providing an affinity capture device. The affinity capture device includes a surface having an aptamer that is at least 80% identical to SEQ ID NO 1 immobilized onto the surface of the affinity capture device. The biofluid sample is diluted with a binding buffer. The binding buffer includes (A) tris(hydroxymethyl)aminomethane (Tris), trimethylamine (TES), 2-ethanesulfonic acid (MES), or 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES); (B) a magnesium cation at a concentration between about 10 µM to about 20 mM; and (C) a total monovalent cation concentration from 0 to no greater than 100 mM. The human immunoglobulin Fc domains in the biofluid sample are adsorbed to the aptamer with the binding buffer.
Abstract: Exemplary embodiments may deploy a valve that introduces a sample of a calibrant coaxially with flow exiting a source of a mobile phase flow, such as a liquid chromatography (LC) column, on a path to an ion source for the mass spectrometer (MS). The valve may be positioned remotely on a branch that has a junction with the path leading form the source of the mobile phase flow to the ion source. Alternatively, the valve may be positioned in line on the flow path from the source of the mobile phase flow to the ion source of the MS. A novel five port valve design may be employed. With this valve design, a first position of the valve allows a sample loop for the calibrant to be filled. In a second position, the calibrant is added coaxially to the flow from the source of the mobile phase to the MS. In a third position of the valve, diversion of or infusion to a post-source flow is enabled.
Type:
Grant
Filed:
April 14, 2021
Date of Patent:
September 19, 2023
Assignee:
Waters Technologies Corporation
Inventors:
Michael O. Fogwill, Joseph D. Michienzi, Sylvain Gilles Cormier
Abstract: The present technology relates to a method of analyzing a sample including an analyte. The method includes injecting the sample including the analyte into a mobile phase. The mobile phase includes a metal chelator additive having a concentration between about 1 ppm to about 10 ppm. The method also includes separating the analyte using liquid chromatography and analyzing the analyte using a mass spectrometer, an ultra-violet detector, or a combination thereof.
Type:
Grant
Filed:
June 5, 2020
Date of Patent:
September 19, 2023
Assignee:
Waters Technologies Corporation
Inventors:
Robert Birdsall, Ying Qing Yu, Jacob Kellett
Abstract: Methods are provided for making rapid labeled dextran ladders and other calibrants useful in liquid chromatography. The methodologies include a two-step process comprising a reductive amination step of providing a reducing glycan and reacting it with a compound having a primary amine to produce an intermediate compound. The intermediate compound is then rapidly tagged with a rapid tagging reagent to produce the rapid labeled dextran ladder.
Type:
Grant
Filed:
October 25, 2019
Date of Patent:
September 5, 2023
Assignee:
Waters Technologies Corporation
Inventors:
Michael F. Morris, Matthew A. Lauber, Darryl W. Brousmiche
Abstract: The present disclosure relates to a method of analyzing a sample comprising a hydrophobic molecule. The method includes preparing an aqueous solution comprising the sample. The method also includes placing the solution in contact with a polypropylene substrate having a deactivated surface that reduces adsorption of the hydrophobic molecule relative to a polypropylene substrate that has not been deactivated. The method also includes analyzing the sample.
Type:
Grant
Filed:
March 3, 2020
Date of Patent:
September 5, 2023
Assignee:
Waters Technologies Corporation
Inventors:
Moon Chul Jung, Mary Elizabeth Lame, Caitlin Dunning, Christopher John Hughes, Xiaoxiao Liu, Paula Orens, Yun Wang Alelyunas, Erin E. Chambers, David Dao, Robert Birdsall
Abstract: Multidimensional liquid chromatography (MDLC) systems include valve configurations that enable a flow containing analytes in the eluent of a first dimension liquid chromatography system to be modulated such that the analytes can be captured in fluidic loops and subsequently provided to a second dimension liquid chromatography system. Optionally, a single detector is used for both first and second dimensions. In addition, the systems can enable dilution of the analytes and enable incompatible mobile phases and mobile phase flow rates to be used in the first and second dimensions.
Type:
Grant
Filed:
May 4, 2021
Date of Patent:
September 5, 2023
Assignee:
Waters Technologies Corporation
Inventors:
Michael O. Fogwill, Sylvain Gilles Cormier, Thomas Swann, Francis Patrick Tracey
Abstract: The present disclosure discusses a method of separating a sample (e.g., small organic acid metabolite) including coating a flow path of a chromatographic system. The coating along the flow path is vapor deposited and prevents or severely decreases metal interactions between the metallic chromatographic system and the sample.
Abstract: The present disclosure is directed to methods for performing size exclusion chromatographic (SEC) separations. Embodiments of the present disclosure feature methods for improved separations of biomolecule analytes, such as CRISPR-related proteins, nucleotides, and ribonucleoprotein complexes, in SEC, for example, by using hydroxy-terminated polyethylene glycol surface modified stationary phase materials and/or C2/PEG surface modified column hardware.
Type:
Application
Filed:
February 14, 2023
Publication date:
August 24, 2023
Applicant:
Waters Technologies Corporation
Inventors:
Lavelay Kizekai, Stephen Shiner, Matthew Lauber
Abstract: Thermally modulated variable restrictors used in chromatography systems enable independent control of system pressure and linear velocity of a compressible mobile phase passing through a chromatography column. A method for configuring a chromatography system with independent control of system pressure and mass flow rate of a compressible mobile phase includes determining a type of chromatography separation column to be used in the chromatography system, matching a thermally modulated variable restrictor to the type of chromatography separation column for use together during operation of the chromatography system, and bundling the chromatography column with its matching thermally modulated variable restrictor for distribution as a single package.
Type:
Grant
Filed:
June 30, 2020
Date of Patent:
August 22, 2023
Assignee:
Waters Technologies Corporation
Inventors:
Michael O. Fogwill, Joseph D. Michienzi, Geoff Gerhardt, James P. Murphy
Abstract: The present invention relates to a method for analyzing a sample, the method comprising: (a) incubating a sample comprising an analyte with at least one enzyme to produce a digestion mixture comprising fragments of the analyte; (b) loading the digestion mixture onto a reversed-phase chromatography column; and (c) performing reversed-phase chromatography on the digestion mixture, wherein the at least one enzyme is hydrophobically modified to increase a retention time of the at least one enzyme such that the at least one enzyme elutes from the reversed-phase chromatography column later than the fragments of the analyte.