Abstract: A reaction bottle which includes a container, container top, container interior a bottle cap removeably attachable to the container top, the bottle cap having a cap top and a cap cavity; a septa attached to the bottle cap and configured to releasably seal the container when the bottle cap is attached to the container top; a needle holder attached to the cap top; a hollow needle attached to the needle holder and located in the cap cavity; a needle conduit in fluid communication with the hollow needle; wherein the septa is configured to deform from an at rest state into a punctured state when pressure within the container interior reaches a first threshold value and the septa deforms into the cavity and is punctured by the hollow needle.