Abstract: The present application describes a large circular target-specific antisense molecule that is effective in ablating RNA and protein expression. The large circular antisense molecule is used to treat any human disease in which modulation of gene expression can be beneficial to intervene in the disease initiation and progression.
Abstract: Large circular (LC)-sense molecules in an array is disclosed. The LC-sense molecules array is combined with cDNA hybridization to detect differences in expression profile between different cells. LC-sense molecules were purified from nonredundant clones with recombinant phagemid and arrayed onto silanized slide glasses. By hybridization of LC-sense array with Cy3 or Cy5-labelled cDNA preparations at 60° C., 29 up-regulated and 6 down-regulated genes in cancerous liver tissue were detected.
Abstract: The present application describes a large circular target-specific antisense molecule that is effective in ablating RNA and protein expression. The large circular antisense molecule is used to treat any human disease in which modulation of gene expression can be beneficial to intervene in the disease initiation and progression.
Abstract: The present invention provides a high-throughput system for functional genomics using a random gene unidirectional antisense library comprising LC-antisense compounds. The antisense compounds were specific and effective for the elimination of target mRNA. Thus, the system of the present invention may be effectively used as temporary knock-down system to unveil functions of genes critical for diseases. The system of the present invention can be adapted not only for functional genomics but also for effectively validating target for antisense or other molecular therapeutics against various malignancies, infections, and other diseases by blocking specific genes involved in the disease.
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