Abstract: The present invention relates to a method for increasing the content of rare ginsenosides in preparing an extract of genus Panax including wild ginseng or ginseng, cambial meristematic cells of genus Panax or an extract thereof, a ginseng extract prepared by the method, cambial meristematic cells (CMCs) of genus Panax or an extract thereof prepared by the method, and to a composition comprising the same.
Type:
Grant
Filed:
August 24, 2015
Date of Patent:
May 12, 2020
Assignee:
WELLKEY HOLDINGS LIMITED
Inventors:
Young-Woo Jin, Eun-Kyong Lee, Young Mi Lee, Il Seok Oh
Abstract: Plant cells express a target protein, a production method thereof, and a method of producing a target protein using the same are disclosed. The plant cells have introduced therein a recombinant vector containing gene(s) encoding a target protein, and the plant cells include plant cambial meristematic cells (CMC) or callus. The cambial meristematic cells (CMCs) are a cell line containing innately undifferentiated cells isolated from a plant, wherein the cell line is a cell line isolated from the cambial tissue of the plant and has meristematic continuity without going through dedifferentiation into callus. A system of expressing a target protein using the recombinant plant cells according to the present invention can overcome the problems of conventional plant cell culture. In addition, it shows significantly high transformation efficiency, and thus can produce large amounts of target proteins, including biopharmaceutical proteins.
Type:
Grant
Filed:
February 28, 2014
Date of Patent:
October 2, 2018
Assignee:
WELLKEY HOLDINGS LIMITED
Inventors:
Young Woo Jin, Eun Kyong Lee, Mi Ok Jang, Bora Park, Soo Ran Lee, Bo Rim Yang, Il Suk Kim, Il Seok Oh