Abstract: An isolation device of target particles from a liquid sample includes an isolation chip, a vacuum unit, and a frequency converting unit. The isolation chip includes a sample reservoir, a first chamber, and a second chamber. The first chamber and the second chamber define a first outlet and a second outlet, respectively. The vacuum unit is connected to the first outlet and the second outlet. The frequency converting unit causes the vacuum unit to generate negative pressures in the first chamber and the second chamber alternately.

Abstract: A device, system, and method for separating, concentrating, and isolating target particles from a bioliquid sample includes a sample reservoir, a first filtration membrane, a second filtration membrane, a first chamber, and a second chamber. The first chamber with first outlet is connected to the sample reservoir through the first filtration membrane. The second chamber with second outlet is connected to the sample reservoir through the second filtration membrane. Negative pressures are applied alternately to the two filtration membranes to isolate target particles, clogging of the membranes being prevented by the same alternating but opposite-phase positive pressures.