Abstract: Methods for producing a bioproduct selected from acetone, isopropanol and a combination thereof with a microorganism in a fermentor are disclosed. The methods include separating cells of the microorganism from a fermentation broth to form separated cells and recycling at least a fraction of the separated cells to the fermentor to achieve one or more of the following: (1) cell concentration in said fermentor greater than 2 g/L; mass yield on a first feedstock greater than 32%; productivity greater than 0.12 g/L/h; and bioproduct titer greater than 10 g/L.

Abstract: Integrated mixotrophic fermentation method comprising (i) an isolated naturally acetogenic organism; (ii) a first feedstock comprising a carbon source for use in a fermentation medium; (iii) a second feedstock comprising elemental hydrogen for use in the fermentation medium; wherein the second feedstock comprises performing electrolysis; and (iv) culturing the organism in the fermentation medium, whereby both feedstocks are metabolized and a fermentation broth is formed, which broth comprises at least one bioproduct.

Abstract: Supplemented mixotrophic method. A mixotrophic fermentation method is disclosed including providing a naturally acetogenic organism; providing a fermentation medium comprising a carbon source and a supplemented non-sugar reductant; and culturing the organism in the fermentation medium, where both the carbon source and the non-sugar reductant are metabolized and a fermentation broth is formed, which contains at least one carbon-containing bioproduct.

Abstract: An integrated wet-mill method for the production of ethanol and single cell protein. An integrated method for the production of ethanol and a single cell protein product is described including providing corn kernels, which kernels comprise germ, fiber, protein and starch and which method includes, among other things, steeping said corn kernels and separating said steeped kernels from a steep liquor.

Abstract: Methods and compositions utilizing organism consisting of the strains within the order Clostridiales for improving health of animals. Disclosed methods comprise administering an effective dose of a composition comprising organism consisting of the strains within the order Clostridiales to an animal, including for example, a broiler or hen, thereby improving said animal's health.

Abstract: Methods for production of a bioproduct with a microorganism and selective extraction of bioproducts from a fermentation broth. The methods may include mixing a carbon source, a nitrogen source, and an extractant-depleted raffinate to form a fermentation medium, and fermenting the medium with a microorganism to form a fermentation broth having at least one bioproduct. The bioproduct may be extracted from the fermentation broth with an extractant comprising an olefin to form an extract and a raffinate, and the extract may be further separated from the raffinate. The bioproduct may then be separated from the extract, and the extractant may be separated from the raffinate to regenerate the extract-depleted raffinate.

Abstract: Provided are dry-milling methods for producing an aqueous fermentation feedstock ingredient. The methods include providing a corn-processing filtration feed; filtering at least a fraction of said filtration feed on a microfiltration membrane, whereby an aqueous filtration permeate and a filtration retentate are formed; separating said filtration permeate from said filtration retentate, to form separated permeate and separated retentate; forming a fermentation feedstock comprising said separated permeate, and saccharifying and/or washing said separated retentate.

Abstract: A method for the production of a rhamnolipid. The method includes culturing in a fermentation medium an organism capable of producing a rhamnolipid to form a fermentation broth comprising at least one rhamnolipid and having a pH>5; extracting at least one lipophilic impurity from said fermentation broth; acidulating said fermentation broth to form an aqueous medium of pH<5 comprising the at least one rhamnolipid; extracting the at least one rhamnolipid from said acidulated fermentation broth; and separating the at least one rhamnolipid to obtain a purified rhamnolipid.

Abstract: The present invention provides microorganisms capable of converting acetyl-coA into crotyl alcohol as well as fermentation methods for producing crotyl alcohol, either alone, or in combination with acetone and/or isopropanol. The microorganisms may be genetically engineered to express and/or disrupt one or more of the following enzymes: acetaldehyde dehydrogenase, alcohol dehydrogenase, bifunctional acetaldehyde/alcohol dehydrogenase, aldehyde oxidoreductase, phosphotransacetylase, acetate kinase, CoA-transferase A, CoA-transferase B, acetoacetate decarboxylase, secondary alcohol dehydrogenase, butyryl-CoA dehydro genase (BCD), and/or trans-2-enoyl-CoA reductase (TER).

Abstract: High cell density anaerobic fermentation for protein expression. A method for producing a protein is described, including providing a modified Butyribacterium methylotrophicum (Bme) strain host; providing a fermentation medium including a carbon source and a nitrogen source; culturing the modified host in the fermentation medium under anaerobic conditions, where the carbon source is metabolized and a fermentation broth including at least one protein is formed and optionally separating the protein; where the Bme cell density in the fermentation broth is greater than 15 g/L.

Abstract: Integrated mixotrophic fermentation method comprising (i) an isolated naturally acetogenic organism; (ii) a first feedstock comprising a carbon source for use in a fermentation medium; (iii) a second feedstock comprising elemental hydrogen for use in the fermentation medium; wherein the second feedstock comprises performing electrolysis; and (iv) culturing the organism in the fermentation medium, whereby both feedstocks are metabolized and a fermentation broth is formed, which broth comprises at least one bioproduct.

Abstract: An integrated wet-mill method for the production of ethanol and single cell protein. An integrated method for the production of ethanol and a single cell protein product is described including providing corn kernels, which kernels comprise germ, fiber, protein and starch and which method includes, among other things, steeping said corn kernels and separating said steeped kernels from a steep liquor.

Abstract: Methods for producing a bioproduct selected from acetone, isopropanol and a combination thereof with a microorganism in a fermentor are disclosed. The methods include separating cells of the microorganism from a fermentation broth to form separated cells and recycling at least a fraction of the separated cells to the fermentor to achieve one or more of the following: (1) cell concentration in said fermentor greater than 2 g/L; mass yield on a first feedstock greater than 32%; productivity greater than 0.12 g/L/h; and bioproduct titer greater than 10 g/L.

Abstract: A method of producing at least one derivate of a carboxylic acid. The method includes reacting a carboxylic acid with an auxiliary alkanol on a first catalyst to form a produced ester. A fraction of the produced ester is optionally separated to form a first separated ester. At least a fraction of said produced ester is reacted with hydrogen on a second catalyst to produce a mixture of product alkanol, auxiliary alkanol and optionally residual ester. The product alkanol is separated from the auxiliary alkanol in the mixture to form separated product alkanol, separated auxiliary alkanol, and optionally a second separated ester. The separated auxiliary alkanol is recycled to the reaction. Methods and catalysts for converting alcohols and acids to hydrocarbon jet and diesel fuels are also described.

Abstract: Disclosed are methods for making one or more bioproducts such as isoprene derivatives, fatty acids, fatty acid derivatives, fatty alcohols, fatty alcohol derivatives, mixtures thereof, and compositions having one or more such bioproducts with microorganisms and for separating the bioproduct(s) or bioproduct composition(s). The methods include providing an extractant having a solvent with a boiling point at atmospheric pressure of less than 20° C.; feeding an aqueous suspension of cells that have produced the bioproduct(s) into a pressurized column; feeding the extractant into the pressurized column; removing an extract having the extractant and the bioproduct(s) from said pressurized column; and separating the extractant from the removed extract to obtain a separated oil-soluble composition having the bioproduct.

Abstract: Mixotrophic fermentation method performed to make one or more bioproducts such as alcohols, organic acids of less than 7 carbons, acetone, 2,3-butanediol and mixtures thereof with a microorganism. The mixotrophic fermentation method includes providing an isolated organism and providing a first feedstock and a second feedstock for use in a fermentation medium. The first feedstock includes a carbon source that is metabolized by the native form of the organism at a rate of less than 0.01 g/hr/g cell mass, and the second feedstock includes CO, CO2, carbonate, bicarbonate, H2, glycerol, methanol, formate, urea or mixtures thereof. The mixotrophic fermentation method also includes culturing the organism in the fermentation medium, whereby both feedstocks are metabolized and a fermentation broth is formed, the broth having at least one bioproduct. Optionally, the bioproduct may be separated from the broth.

Abstract: Methods for production of a bioproduct with a microorganism and selective extraction of bioproducts from a fermentation broth. The methods may include mixing a carbon source, a nitrogen source, and an extractant-depleted raffinate to form a fermentation medium, and fermenting the medium with a microorganism to form a fermentation broth having at least one bioproduct. The bioproduct may be extracted from the fermentation broth with an extractant comprising an olefin to form an extract and a raffinate, and the extract may be further separated from the raffinate. The bioproduct may then be separated from the extract, and the extractant may be separated from the raffinate to regenerate the extract-depleted raffinate.

Abstract: Methods for production of a bioproduct with a microorganism and selective extraction of bioproducts from a fermentation broth. The methods may include mixing a carbon source, a nitrogen source, and an extractant-depleted raffinate to form a fermentation medium, and fermenting the medium with a microorganism to form a fermentation broth having at least one bioproduct. The bioproduct may be extracted from the fermentation broth with an extractant comprising an oxygenated organic compound and a hydrocarbon to form an extract and a raffinate, and the extract may be further separated from the raffinate. The bioproduct may then be separated from the extract, and the extractant may be separated from the raffinate to regenerate the ex tract-depleted raffinate.

Abstract: Provided are methods, systems and apparatuses to the energy efficient and selective extraction of biomolecules, e.g., small organic compounds, e.g., one or more C3-C9 alcohols, one or more C3-C5 carboxylic acids, and mixtures thereof, from an aqueous solution, particularly aqueous solutions containing the alcohol in dilute or low concentrations, for example, fermentation broths.

Abstract: Provided are methods, systems and apparatuses to the energy efficient and selective extraction of biomolecules, e.g., small organic compounds, e.g., one or more C3-C9 alcohols, one or more C3-C5 carboxylic acids, and mixtures thereof, from an aqueous solution, particularly aqueous solutions containing the alcohol in dilute or low concentrations, for example, fermentation broths.